Micropropagation of Mokara Orchid by Temporary Immersion System Technique

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International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume VII, Issue V, May 2022 | ISSN 2454–6194

Micropropagation of Mokara Orchid by Temporary Immersion System Technique

 Tran Van Minh
International University, Vietnam National University Ho Chi Minh City, Vietnam

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Abstract: Based young leaves of Mokara Leuen Berger Gold were used as cultured materials. Callus was initiated and increase biomass on medium of semisolid and liquid cultures: MS medium supplemented with CW (30%), sucrose (30 g/l), 2.4D (1 mg/l). Callus was used as materials for initiation and biomass propagation on medium: (1) MS medium supplemented with NAA (1 mg/l), B1 (5 mg/l), Adenin sulfate (10 mg/l), peptone (1 g/l), CW (10%), sucrose (30 g/l) (2) MS medium supplemented with 2.4D (1 mg/l), CW (30%), Adenin sulfate (10 mg/l), peptone (1 g/l), CW (10%), sucrose (30 g/l). Semisolid medium for callus cell regeneration and to induce multiple shoots were: MS medium supplemented with BA (0.5 mg/l), B1 (5 mg/l), Adenin sulfate (10 mg/l), peptone (1 g/l), CW (10%), sucrose (20 g/l). Multiple-shoots were propagated on MS medium supplemented with BA (0.5 mg/l), B1 (5 mg/l), CW (10%), sucrose (20 g/l). Propagation of multiple-shoots in TIS on MS medium supplemented with BA (0.5 mg/l), B1 (5 mg/l), CW (10%), sucrose (20 g/l). Interval culture time was optimum for 2 hours floating and 1 minute rising. Plantlets were induced roots on MS medium supplemented with NAA (1 mg/l), B1 (5 mg/l), CW (10%), sucrose (20 g/l). Experiments on callus formation and callus growth of mokara orchids both on semi-solid medium (agar) and liquid medium to create callus suspension, the best medium selected was: MS supplemented with 2.4D (1 mg/l), CW (30%), sucrose (30 g/l)

Keywords: Bioreactor temporary immersion system, callus, callus cell suspension, multi-shoots

I.INTRODUCTION

Orchids are a beautiful flower with high economic value, currently having a strong domestic and export market. Some flower varieties can be grown in the humid tropical conditions of Ho Chi Minh City such as: Dendrobium, Mokara, Phalaenopsis, Cattleyas, Vandaceous, Oncidium… In which, the cut branches of orchids belonging to the Mokara and Dendrobium groups are grown by many households and have a high profit rate despite the high initial investment, especially seed investment.