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International Journal of Research and Innovation in Applied Science (IJRIAS) |Volume VII, Issue XI, November 2022|ISSN 2454-6194

Phytochemical Screening, Antimicrobial Activities and Gas Chromatography Profile of the n-Hexane extract of Trigonella foenum-graecum “Fenugreek” Seed Oil.

Edah Oba Alexander1, Awode Udukhomo Anthony2*, Azike Ruth2, Jidimma Joel Augustine2
1Faculty of Pharmaceutical Sciences, University of Jos. Nigeria.
2Department of Chemistry, University of Jos, Nigeria.
*Corresponding Author

IJRISS Call for paper

Abstract: To evaluate the phytochemical constituents, antimicrobial activity, and GC profile of the seed oil extract of Trigonella foenum-graecum. In the present research, solvent extraction by maceration was performed by using 95% n-Hexane. Phytochemical analysis of the n-Hexane extract was carried out. Phytochemical screening showed that alkaloids, tannins, flavonoids, carbohydrates, balsam, and phenols were negative, while saponins, resins, terpenes, steroids and cardiac glycosides were positive. The Antimicrobial potential of Trigonella foenum-graecum seed oil extract was determined using the agar well diffusion method by the zone of inhibition on nutrient agar. MIC and MBC tests were performed to determine the relative antimicrobial properties of the extract. GC profile was also carried out to determine the constituents in the oil extracts. For the agar, well diffusion method of antimicrobial activity screening, all the concentrations of Trigonella foenum-graecum showed negative results in all the bacteria except for Bacillus subtilis. The extract is highly selective in terms of antimicrobial potency; the isolation of the active component(s) may present more insight. For the GC profile, 11 peaks were recorded.

Keywords: Trigonella foenum-graecum, agar well diffusion method, bacterial strains, inhibition.


Plant extracts are treasured based on their ethnomedicinal values. Their various intrinsic pharmaceutical, cosmetic, and nutraceutical properties further define their economic roles for entrepreneur purposes. Fenugreek is one plant that possesses phytoconstituents such as flavonoids, alkaloids, terpenoids, steroids, saponins, anthocyanins, and tannins (Sumayya et al., 2012). The evolution of resistant strains of microorganisms to multiple antibiotics in combination therapy has generated great interest for research into plants with active compounds that elicit therapeutic, medicinal, aromatic, and aesthetic uses Gurinder and Daljit et al., (2009), Newman and Cragg et al., (2007). Phytochemicals are natural and non-nutritive bioactive compounds produced by plants, which act as protective agents against external stress and pathogenic attack. It could be administered singly or combined with other active agents in the formulation of drugs. Rashmi et al., (2011). Infectious diseases and drug resistance have raised serious