Antibiotic Susceptibility of Bacterial Isolates from Pus Specimens Collected from a General Hospital in Dhaka, Bangladesh

International Journal of Research and Scientific Innovation (IJRSI) | Volume VIII, Issue VII, July 2021 | ISSN 2321–2705

Antibiotic Susceptibility of Bacterial Isolates from Pus Specimens Collected from a General Hospital in Dhaka, Bangladesh

Sumona Rahman Shewly¹, Mosa. Hafsa Akter Ane¹, Tamanna Zerin^1*, Md. Altaf Hossain^2
1Department of Microbiology, Stamford University Bangladesh, 51, Siddeswari Road, Dhaka-1217, Bangladesh
²Insaf Barakah Kidney and General Hospital, 11, Shahid Tajuddin Ahmed Sharani, Dhaka-1217, Bangladesh
*Corresponding author

Abstract: Antibiotic resistance and its rapid spread among pathogenic bacterial isolates are regarded as major public health issues around the world. The study aims to detect pyogenic bacteria in pus samples and assess their antibiotic susceptibilities to various antibiotics commonly employed in chemotherapeutic interventions. This is a retrospective study in which 297 pus samples were obtained from patients in the year 2018 to 2020 for aerobic culture and sensitivity testing. In total, 32% (95) of the samples yielded positive results, whereas, 68% (202) yielded negative results. The most commonly occurring pathogen was E. coli (65) followed by Pseudomonas spp. (14), Staphylococcus aureus (9), Klebsiella spp. (4) and Acinetobacter spp. (3). Patients aged 51 and up were the ones who were largely afflicted by the pus infection. Males were shown to be more susceptible to infection than females. Amikacin, Ceftazidime, Meropenem, Cefotaxime, Netilmicin, Mecillinam, and Fosfomycin were the most susceptible drugs for Klebsiella spp., followed by Meropenem for E. coli, Cloxacillin, Doxacillin, Cefotaxime, and Amoxiclave for S. aureus. However, Pefloxacin and Amoxiclav were both effective against Pseudomonas spp., while Levofloxacin, Ciprofloxacin, Doxacillin, Tazobactam+Piperacillin, Imipenem, Netilmicin, and Trimethoprim were effective against Acinetobacter spp. The antibiotic, Ampicillin was found to be resistant to all of the microorganisms tested. In general, most of the isolates were found to be resistant to the majority of the antibiotics. As a result of our research, clinicians will be able to make better decisions when selecting appropriate antibiotics, which aid in patient care and judicious usage, preventing the development of drug resistance in those who are already susceptible.

Key words: Pus, Antibiogram, Age, Gender, Bangladesh

I. INTRODUCTION

Pus is usually caused by a bacterial infection that is contained in an abscess that can be developed anywhere in the body. Furthermore, virus, protozoa, fungus can also cause wound infection, and sometimes, they simultaneously causes infection with one or more bacteria in a distinct wound [1]. Typically, it looks like white to yellow fluid made up of dead neutrophils, cellular waste, and necrotic tissues. Pus is formed when microbial pathogens cause human skin and soft tissue infections (SSTIs) during or after trauma, burn injuries, and surgical procedures [2-4]. Hospital-acquired wound infections may cause severe morbidity, and prolonged hospitalization that may pose a considerable monetary burden [5]. Both aerobic and anaerobic, gram-positive and gram-negative bacteria are implicated in pus infection. A number of commonly involved bacterial species in pus formation includes Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Escherichia coli, Streptococcus pneumoniae

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