Antifungal Activity of Local Gin (Kai Kai) Extract of Andrographis Paniculata on Fungal Isolates

International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume V, Issue VI, June 2020 | ISSN 2454-6194

  Antifungal Activity of Local Gin (Kai Kai) Extract of Andrographis Paniculata on Fungal Isolates

Robinson,V. K, Nnamdi, A. U, Korobe, B. P.
Department of Microbiology, Rivers State University, Nkpolu-Oroworukwo, PMB 5080, Port Harcourt, Nigeria

Abstract:- A. paniculata(king of bitters) an herbaceous plant known for its bitter taste and medicinal properties has been used in treating different forms of infections and ailments. The antifungal activity of A. paniculata on fungi isolated from Citrullus colocynthis was investigated. Colocynthiscitrullus were bought from traders in a major market in Abia State, Nigeria. Fresh plant of A. paniculata was obtained from botanical garden of the Rivers State University and was identified in the botany department. The leaves of the plant were shade dried and blended into fine powder. Twenty grams(20g) of the powdered leaves was extracted using local gin ‘kai kai’. The filtrate was evaporated and the resulting crude extract was used for antifungal sensitivity. The antifungal activity of the extracts was carried out using the well in agar diffusion method. In this method, 48 hours old fungal isolate was inoculated on dried Sabouraud Dextrose Agar plates in duplicates. five wells were bored using sterile 6mm cork borer on the dried seeded plates before 0.2ml of the different concentrations of 100, 50, 25, and 12.5mg/ml of the methanol extracts were transferred into the wells using sterile pipettes. Aspergillus flavus, Rhizopus arrhizus, Aspergillus niger, and Mucor sp were identified from the melon seeds. The zone diameter of the alcoholic extract of A. paniculata on A. niger was 16.50±0.71, 13.50±0.71, 10.50±0.71 and 0.00±0.00mm at the concentrations of 100mg/ml, 50mg/ml, 25mg/ml and 12.5mg/ml, respectively. While for A. flavus, the zone diameters were 33.50±0.71, 21.50±0.71, 19.50±0.71, and 0.00±0.00mm, respectively. The extract had no antifungal activity on Rhizopus arrhizu sand Mucor sp. Theantifungal activity of the extract was greatly influenced by the concentration of the extract, with higher concentrations of extract having high zone diameter.
Key words: Local gin ‘kai kai’ A. paniculata, antifungal activity, fungal isolates

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