International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume VII, Issue VII, July 2022 | ISSN 2454–6194
Industrial Propagation of Cymbidium Sp. by Bioreactor Technique
Tran Van Minh
International University, Vietnam National University HCM, Ho Chi Minh City, Vietnam
National Key Lab for Plant Cell Biotechnology, Ho Chi Minh City, Vietnam
Abstract: Micropropagation of orchid plant for conservation and development is needing. Traditional propagation of Cymbidium sp. requires energy cost, many labor, large area for growing, slow growth and development, and high cost input. It is a need to find new effective ways for in vitro propagation, and plant cell technology via bioreactor techniques effort the demands. Protocorm like bodies were used as planting materials. Somatic embryo callus were initiated on medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l) or 2,4D (1 mg/l). Somatic cell suspension were cultured for initiation and for proliferation. on medium MS + BA (0.1mg/l) supplemented with 2.4D (1 mg/l) and NAA (1 mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. Somatic embryo suspension were cultured in bioreactor for initiation and proliferation on the medium MS + BA (0.1mg/l) supplemented with 2.4D (1 mg/l) or NAA (1 mg/l). Embryogenic suspension was stimulated on the medium MS + BA (0.5 mg/l) + NAA (0.1 mg/l). In vitro shoots of Cymbidium sp. were regeneration on the medium MS + BA (0.1 mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/6hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favoured at 26+2oC, 11,1-22,2 μmol/m2/s, and 30 rpm. Micropropagation of Cymbidium sp. by bioreactor technique was set up to produce 5,600 platlets per one liter of somatic embryogenesis suspension.
Keyword: bioreactor, Cymbidium sp., homogenous cell, industrial propagation, somatic embryogenesis, temporary immersion system (TIS) bioractor,
I. INTRODUCTION
Traditional micropropagation [1] on orchids currently leads to a problem that micropropagation laboratories often face, which is that tissue culture plants often grow slowly, are very labor-intensive, and costly. It takes a long time to produce seedlings in large quantities when marketed at a high cost of seedlings. The embryo cloning system solves the above barrier with the advantages: rapid multiplication in the form of cells, cloned embryo is a differentiated organism with high regeneration coefficient, low labor and low energy cost [2]. In somatic embryo technology, liquid culture is the basic technique performed on shakers or bioreactors [3,4] with the aim of increasing biomass, inducing homogenous somatic embryogenesis and leading on the ability to regenerate somatic embryos with high efficiency [5].