Industrial Propagation of Phalaenopsis sp. by Bioreactor Technique

International Journal of Research and Innovation in Applied Science (IJRIAS) |Volume VIII, Issue IV, April 2023|ISSN 2454-6194

Industrial Propagation of Phalaenopsis sp. by Bioreactor Technique

Tran Van Minh1,2*
1International University, Vietnam National University Ho Chi Minh City
2Key Lab of Plant Cell Biotechnology, Institute of Tropical Biology
*Corresponding author
DOI: https://doi.org/10.51584/IJRIAS.2023.8404
Received: 14 February 2023; Accepted: 16 March 2023; Published: 26 April 2023

Abstract: Protocorm like bodies (PLB) were used as planting materials. Somatic embryo callus were initiated on medium supplemented with NAA (1mg/l) or 2.4D (1mg/l). Somatic cell suspension were cultured for initiation and for proliferation. on medium MS supplemented with NAA (1mg/l) and NAA (0.5mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. Somatic embryo suspension were cultured in bioreactor for initiation and proliferation on the medium MS supplemented with NAA (0.5mg/l). Embryogenic suspension was stimulated on the medium MS supplemented with BA (0.5mg/l) + NAA (0.1mg/l). In vitro shoots of phalaenopsis were regeneration on the medium MS supplemented with BA (0.5mg/l) + NAA (0.1mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favored at 26+2oC, 11,1-22,2μmol/m2/s, and 30rpm. Micropropagation of phalaenopsis sp. by bioreactor technique was set up to produce 7,580 plantlets per one liter of somatic embryogenesis.

Keyworks: Phalaenopsis sp., somatic embryogenesis, micropropagation, protocorm like bodies, bioreactor technique.

I. Introduction

Traditional breeding micro [1] on orchids leads to a problem that the common micro -breeding laboratories were that the tissue transplantation often grows slowly, costs a lot of labor costs, lot of time to produce seedlings in large quantities when marketed at a high price of seedlings. Asexual breeding system [2] solves the above barrier with the advantages: fast multiplying in the form of cells, asexual embryo is a different differentiation of regeneration coefficient, low labor costs and low cost [3]. In soma embryo technology, liquid culture was a basic technique made on a shake or Bioreactor [4,5] aims to increase biomass, induction that generates a homogeneous and conductor to the ability to regenerate soma with high performance [6], orchids [7]. Bioreactor technique has been researched and applied to the breeding microwave to reduce the cost of tissue transplanting products [3]. Cultivation materials in micro propagation with Bioreactor technology such as embryonic callus, cloned embryo cells, protocorms, shoots [7]. There are also many types of bioreactors used for micro-multiplication such as Bioreactor of cylindrical gas (Airlift Bubble Column-Bioreactor, Bioreactor Bubble Balloon-Balloon-Bioreactor, Bioreactor Footer Tank-Bioreactor) Contemporary Bioreactor [6]. Each type of Bioreactor has different features, depending on the physiological properties of farming plants, aiming to increase biomass quickly and enhance growth. Physical and chemical factors are factors that greatly affect cell proliferation and affect cell regeneration later [8]. Successfully taken through soma embryo culture on orchids [9] and micropropagation via bioreactor and temporary immersion system of date palm [11], orchid [12], lily [13], ruber [14], cocoa [15]. This paper studies the rapid multiplication of coffee plants using bioreactor technology.
This article studied propagation of orchids with Bioreactor technology on large scale.

II. Materials and Methods

Materials

Varieties: Phalaenopsis orchids imported from Singapore (Phalaenopsis sp).
Explants: Young leaf sheath, PLB

Methods

The culture mineral nutrient medium is MS [10]. Added: BA (6-benzylaminopurine), TDZ (thidiazuron), IBA (β-indol butyric acid), NAA (α-naphthalene acetic acid), adenine, kinetin (6-furfurylaminopurine), B1 (10mg/l), peptone (1g/l), coconut water (10%), sucrose (30g/l), activated carbon (1g/l).

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