International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume VII, Issue VI, June 2022 | ISSN 2454–6194
Industrial Propagation of Rhynchostylis Sp. By Bioreactor Technique
Tran Van Minh
International University, Vietnam National University HCM, Ho Chi Minh City, Vietnam
National Key Lab for Plant Cell Biotechnology, Ho Chi Minh City, Vietnam
Abstract: Rhyncostylis sp. propagation by multi-shoot system induction from meristem culture in invitro. It take more labor, energy, large area, and high cost. Plant cell technology is effective way for micropropagation in bioreactor. Protocorm like bodies were used as planting materials. Somatic embryo callus were initiated on medium MS + IAA (1.0 mg/l). Somatic cell suspension were cultured for initiation and for proliferation on medium MS + 2.4D (0.5 mg/l) + kinetin (1 mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. Somatic embryo suspension were cultured in bioreactor for initiation and proliferation on the medium MS + NAA (0,5 mg/l) + 2.4D (1 mg/l). Embryogenic suspension was stimulated on the medium MS + NAA (0.3 mg/l) + BA (0.3 mg/l). In vitro shoots of Rhynchostylis were plating and regeneration on the medium MS + NAA (0,1 mg/l) + BA (0,5 mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favoured at 26+2oC, 11,1-22,2 μmol/m2/s, and 30 rpm. Micropropagation of Rhynchostylis sp. by bioreactor technique was set up to produce 6,800 plantlets per one liter of somatic embryogenesis suspension.
Keywords: Rhynchostylis sp., protocorm like bodies, embryogenic callus, somatic embryogenesis suspension
I. INTRODUCTION
Traditional micropropagation [1] on orchids currently leads to a problem that micropropagation laboratories often face, which is that tissue culture plants often grow slowly, are very labor-intensive, and costly. it takes a long time to produce seedlings in large quantities when marketed at a high cost of seedlings. The embryo cloning system [2] solves the above barrier with the following advantages: rapid multiplication in the form of cells, the cloned embryo is a differentiated organism with high regeneration coefficient and low cost. labor costs and lower costs [3]. In somatic embryo technology, liquid culture is the basic technique performed on shakers or bioreactors [4,5] with the aim of increasing biomass, inducing homogenous somatic embryogenesis and leading to high efficiency somatic embryo regeneration [6,7]. Bioreactor techniques have been studied and applied to micropropagation in order to reduce the cost of tissue culture products [3]. Culture materials in micropropagation by bioreactor technology such as embryogenic callus cells, clonal embryonic cells, protocorm, bud clusters [7].