Inoculation of Sulphate Reducing Bacteria (SRB) in Crude Oil from Oil Producing Wells in Niger Delta, Nigeria
- September 18, 2020
- Posted by: RSIS Team
- Categories: Biochemistry, IJRIAS
International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume V, Issue III, January 2020 | ISSN 2454–6186
Chikwe T.N.
University of Port Harcourt, Petroleum and Environmental Chemistry Research Group Department of Pure and Industrial Chemistry, PMB 5323 Choba, Port Harcourt, Nigeria
Abstract: – Water produced from three crude oil samples obtained from the Niger Delta area of Nigeria were inoculated to determine the presence of Sulphate Reducing Bacteria (SRB). Produced water from samples A, B and C were obtained by spinning the crude samples using a Rotanta Petroleum Centrifuge set at 40 0C and 1500 rpm. 1 ml of the inoculum (produced water) from each of the crude samples was introduced into six inoculating test bottles containing saline solution of six different concentrations 100, 10-1, 10-2, 10-3, 10-4, 10-5 g/mol respectively with the aid of six syringes by serial dilution. The six inoculated test bottles were incubated in an incubator set at a temperature between 37 – 40 0C for 28 days with each bottle observed each day. The presence of Sulphate Reducing Bacteria (SRB) was confirmed by the appearance of a black residue in each test bottle and the quantity of Sulphate Reducing Bacteria (SRB) in colony forming unit is determined by the concentration of the inoculating medium the bacteria appeared. The lower the concentration of the inoculating medium, the higher the bacteria count in colony forming unit per mol (CFU/mol). Results obtained showed that Sample A has a bacterial count of <10 CFU/mol, Sample B has a bacterial count between 100 – 1000 CFU/mol while Sample C has a bacterial count between 1 – 10 CFU/mol. Inoculation of SRB in crude oil samples is very important in determining the bacterial count in crude samples and this is critical in understanding the concentration and frequency of bactericides required in the treatment of Sulphate Reducing Bacteria.
Key Words: – Bactericide, sulphide, inoculation, saline, concentration, temperature.
