Isolation and Identification of Microorganisms Associated With Substrate Used For Biogas Generation
- January 15, 2021
- Posted by: RSIS Team
- Categories: Applied Science, IJRIAS
International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume V, Issue XII, December 2020 | ISSN 2454–6186
Isolation and Identification of Microorganisms Associated With Substrate Used For Biogas Generation
Uchendu, D.O. and Mbonu, F.O.
Department Of Science Laboratory Technology, Akanu Ibiam Federal Polytechnic, Unwana, Afikpo, Ebonyi State, Nigeria
ABSTRACT:- Microorganisms play a vital role in processing organic matter and returning the chemical elements into active cycle. In these, decomposers are effective in dismantling the complex organic matter through a sequential breakdown and release of energy. Biogas is one such process which occurs in the absence of oxygen and several groups of microorganisms involved in degrading the organic compounds only releases methane gas, to attain biogas with high methane concentration, it is important to treat and maintain the suitable microorganisms involved within the digester. Therefore, the research was carried out to isolate and characterized microorganisms associated with biogas generation from cow dung and kitchen wastes before anaerobic digestion and to determine the proximate composition of the substrates. The proximate composition of the substrates were determined according to official method of association of official analytical chemist (2000). The result of proximate composition of the substrates to be use in this research shows that moisture content of cow dung was 7.23 ± 0.18 and kitchen 8.1 ± 0.16, ash contents 2.73 ± 0.3 and 3.24 ± 0.1, crude protein content 14.19 ± 0.6 and 17.1 ± 0.2, crude fiber content 1.08 ± 0.08 and 2.02 ± 0.7, fat content 0.63 ± 0.02 and 0.8 ± 0.04, carbohydrate content 7.0 ± 0.6 and 8.2 ± 0.7 respectively. Microbiological analysis were carried out using standard microbiological procedures. Total heterotrophic bacteria and fungi count indicate that cow dung had 7.46 x 10–5 (cfu/ml) kitchen waste 5.82 x 10–5 (cfu/ml). For fungi count 6.04 x 10–5 (cfu/ml) and 4.93 x 10–5 (cfu/ml), respectively. The result revealed that 12 heterotrophic bacteria which includes Pseudomonas spp, Escherichia coli, Bacillus spp, Salmonella spp, Staphylococcus aureus, Serratia spp, Shigella spp, Micrococcus spp, Proteus vulgaris, Citobacter spp, Kelbsiella spp and 4 fungi which includes; Fusarium spp, Mucor spp, Aspergillus spp and Penicillium spp where isolated from the substrates.
INTRODUCTION
The most fascinating features of any civilized communities are the abundant availability of energy for domestic, agricultural and industrial purposes (1). Biogas is produced when bacteria degrade biological materials in the absence of oxygen, in a process known as anaerobic digestion (2). Anaerobic treatment is the use of biological processes, in the absence of oxygen, for the breakdown of organic matter and the stabilization of these materials by conversion to methane and carbon dioxide gases and a nearly stable residue (3). Animal wastes and kitchen wastes can be used as sources of nutrient, feed ingredients to microorganisms and as fuel energy source, they contain high level of organic matter that could be converted into energy as supplement for fossils. Animal wastes are abundant all over the world with Nigeria producing about 227,500 tons of fresh waste each day (4).
