Phytochemical Screening, GC-MS Analysis, Toxicity and Antimicrobial Properties of Extracts Outer Shell Poikilospermum suaveolens (Blume) Merr

International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume VI, Issue IX, September 2021|ISSN 2454-6194

Phytochemical Screening, GC-MS Analysis, Toxicity and Antimicrobial Properties of Extracts Outer Shell Poikilospermum suaveolens (Blume) Merr

Abdul Hapid^1,2*, Mery Napitupulu³, and Muhammad Sulaiman Zubair^4
1Faculty of Forestry, Tadulako University, Jl. Soekarno Hatta Km 9, Bumi Tadulako, Tondo, Palu 94118, Central Sulawesi, Indonesia
²Doctor of Agricultural Science Study Program, Postgraduate Program, Tadulako University. Jl. Soekarno Hatta Km 9, Bumi Tadulako, Tondo, Palu 94118, Central Sulawesi, Indonesia
³Science Department, Faculty of Teacher Training and Education, Tadulako University. Jl. Soekarno Hatta Km 9, Bumi Tadulako, Tondo, Palu 94118, Central Sulawesi, Indonesia
⁴Pharmacy Department Faculty of Math and Science, Tadulako University, Palu 94148, Indonesia
*Correspondence author

Abstract: Poikilospermum suaveolensis a medicinal plant used by local communities, especially the Kaili tribe around Lore Lindu National Park, Sigi Regency, Central Sulawesi Province, Indonesia for the treatment of breast cancer and jaundice. Because of the importance of this plant, this study was designed to determine the content of secondary metabolic compounds, toxicity, antimicrobial properties and identify the compounds through Gas chromatography mass spectrometry (GC-MS) analysis. The results of the study found that the outer skin extract of P. suaveolens contained alkaloids, tannins, saponins and steroids. The extract showed high toxicity with an LC50 value of 44.41±25.71 m/mL. Antimicrobial activity test showed that the extract with a concentration of 25% had the inhibition of bacterial growthStaphylococcus aureus 16.17±0.29 mm (strong), Escherichia coli bacteria 14.50±0.50 mm (strong) and Candida albicans at 17.00±1.00 mm (strong). AGas chromatography mass spectrometry (GC-MS) analysis found that there were about 50 compounds in the outer shell extract of P. suaveolens including Lupan-3-Yl Acetate, 1,3,4,5-Tetrahydroxy-Cyclohexanecarboxylic Acid, Dl-Threitol, 6-Octadecenoic Acid, (Z)-, L-(+)-Ascorbic Acid 2,6-Dihexadecanoate, 5-Hydroxy-7-Methoxy-2-Pentylchromone, Vitamin E, 3-Methoxy-5-Methylphenol, 4,6-Dioxatricyclo[ 3.3.1.02,7]Nonane, 1,3,3-Trimethyl-, (1s)-, Ammoniumchlorid, Tetraoctyl-. This study found that this extract has the potential to be used as an antimicrobial source from safe natural ingredients

Keywords:, Poikilospermum suaveolens, Phytochemicals, Toxicity, Antimicrobials, GC-MS

I. INTRODUCTION

The world is facing a very crucial problem with the presence of Covid-19 which has spread throughout the world including Indonesia (Huo et al., 2021). The dilemma of various countries in tackling COVID-19 has made the government and even the public feel anxiety and losses that have an impact on the economy and health(Gulseven et al., 2020).

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