Study of Ethanolic Extract of Parkiabiglandulosa and It’s Isolate Targeting Angiogenesis

International Journal of Research and Scientific Innovation (IJRSI) | Volume V, Issue III, March 2018 | ISSN 2321–2705

Study of Ethanolic Extract of Parkiabiglandulosa and It’s Isolate Targeting Angiogenesis

Swati Dhande, Prakash Chaudhary, Sneha Agrawal

 Department of Pharmacology, BharatiVidyapeeth’s College of Pharmacy, Sector-8, C.B.D. Belapur, Navi Mumbai, India

ABSTRACT: – Angiogenesis, the sprouting of new blood vessels from pre-existing, vessels is a crucial process for tumor growth and metastasis. It is a physiological process required for wound healing, repair, embryonic development. Immediately after injury, angiogenesis is initiated by multiple molecular signals, including pro-angiogenic factors, angiopoietins, inflammation, cytokine growth factors, and cell-matrix interactions. Imbalance in this factors leads to disease condition like excessive angiogenesis in tumour. Different parts of Parkiabiglandulosa shows activity like anti ulcer, anti inflammatory, anti bacterial, anti fungal. Based on composition of different phytoconstituents like gallic acid, lupeol, campestrol and β-sitosterol plant was selected for the targeting of angiogenesis. β-sitosterol was isolated from crude extract by using different solvents on flash chromatography. In this study anti angiogenic effect was studied at low and high doses of EEPB (ethanolic extract parkiabiglandulosa), its isolate β-sitosterol and standard imatnibmesylate was studied on regenerational and developmental model of zebrafish and their embryos.

Keywords: Angiopoietin, Pro-angiogenic, β-sitosterol, developmental, regenerational, zebrafish.

I. INTRODUCTION

Angiogenesis is a process of generating new capillary blood vessels i.e. neo vasculature, that occurs through sprouting of pre-existing vessels which play a crucial role during embryonic development, growth and healing. It is a complex process wherein there is extensive interaction between cells, soluble factors and extra cellular matrix (ECM) components.[1] The process of angiogensis involves various factors like pro angiogenic factor which includes vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), platelet derived growth factors (PDGF), angiopoietins, and anti angiogenic factors includes angiostatin, restin, arresten, canstatin and tumstatin etc.[2]-[6] Any imbalance in these factors leads to abnormal angiogenesis which is involved in many pathological processes including tumour growth, metastasis, age related macular degeneration, psoriasis, endometriosis, arthritis. Thus targeting angiogenesis may help to combat with such diseases. In cancer angiogenesis is major event in growth of tumour and metastasis; thus excessive proliferation of blood capillaries is crucial for supply of proper nutrients and oxygen. The cancer cells sends signal to the normal surrounding tissues which activate certain genes in tissue cells and encourages the formation of new blood vessels in tissue which have lost control of cell cycle in those affected tissues. [7]

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