Thin Cell Layer Culture of Phalaenopsis sp. and Rhynchostylis sp.

Thin Cell Layer Culture of Phalaenopsis sp. and Rhynchostylis sp.

Tran Van Minh
International University, Vietnam National University HoChiMinh City
Key Lab of Plant Cell Biotechnology, Institute of Tropical Biology

DOI: https://doi.org/10.51584/IJRIAS.2023.8504

Received: 11 April 2023; Revised: 25 April 2023; Accepted: 01 May 2023; Published: 30 May 2023

Abstract: Phalaenopsis sp: The corm of shoot tip from elite shoot 90-day-old was used as material on the medium of MS + BA (1mg/l) + NAA (0.1mg/l) to rise to 2-4 PLB/corm after 90 days cultivation. PLBs were used as materials for in vitro propagation. PLB was cut into thin slices and cultures on the PLB initiation medium MS + peptone (1g/l) + BA (1mg/l) + NAA (0.1mg/l). After 3 months, PLB was initiated on different samples with 11.8 PLB/corm shoot tip sample, 7.4 PLB/leaf sample, and 6.2 PLB/thin slice of PLB. PLB has multiplied on the medium MS + BA (1mg/l) + NAA (0.1mg/l) to rise 11,4 PLB/sample. PLB was regenerated and rooted on the medium MS + NAA (0.1mg/l) with a high rate of 100%. The thin cell layer culture of Phalaenopsis sp. was established.
Rhynchostylis sp: Shoot in 60-day-old clone in the pot was used as material for set up in vitro shoots by cultured on the medium MS + 2iP (1mg/l) + IBA (0,5mg/l) to raise 4-6 shoot/sample after 30 days of cultivation. Young shoots were multiplied on the medium MS + BA (1mg/l) + IBA (0.1mg/l) to produce 5-8 shoots/sample. The young shoot released young leaves and corms of shoot tip meristem used as material in thin cell layer culture. The corm shoot tip meristem was cultured on medium MS + BA (1mg/l) + IBA (0,5mg/l) to rise 4.8 shoots/sample; and young leaves were cultured on medium MS + 2iP (1mg/l) + IBA (0,5mg/l) to rise 2.4 PLB/sample and 3.6 shoots/sample. Shoots were multiplied on medium MS + BA (1mg/l) + IBA (0,5mg/l) and PLB was regenerated and rooted on medium MS + IBA (0,5mg/l). The thin cell layer culture of Rhynchostylis sp. was established.

Keywords: Phalaenopsis sp., Rhynchostylis sp. somatic embryogenesis, micropropagation, protocorm-like bodies, bioreactor technique.

I. Introduction

Mineral composition and plant growth regulators play an important role in the growth and development of plants, especially in vitro plants [1]. Mineral nutrient composition in each environment has different nutrient compositions and concentrations. Each plant species is suitable for a certain composition and concentration of mineral nutrients. Plant growth regulators are commonly used in vitro to regulate homeostasis and control morphogenesis, organogenesis in vitro [2]. The balance of growth regulators determines the in vitro culture process [3].

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