Genetic Diversity Analysis of Pearl Millet (Pennisetum Glaucum [L. R. Rr.]) Accessions from Northwestern Nigeria

Pearl millet, a critically drought-tolerant cereal supporting millions in Northern Nigeria, possesses significant unexplored genetic diversity. This study addressed the research gap in north-western Nigeria by analysing the genetic diversity and evolutionary relationships of 69 pearl millet accessions using 8 polymorphic Simple Sequence Repeat (SSR) markers. The analysis confirmed substantial molecular diversity, revealing a total of 53 alleles with an average of 6.875 per locus, ranging from 3 (PSMP2248) to 9 (PSMP2080) alleles. Both factorial and phylogenetic cluster analyses demonstrated that genetic grouping was based on molecular similarity rather than geographical origin. The resulting clusters were small and exhibited high internal similarity, suggesting a history of seed exchange or shared ancestry among accessions. These findings confirm the rich genetic diversity present within the region's pearl millet populations. The study recommends further investigation using additional molecular markers to provide a more comprehensive understanding of the genetic resources available for this vital, under-researched crop.

pearl millet, genetic diversity, simple sequence repeats (SSR)

1. Abdulhakeem, A., Ahmed, F. O., Omoniyi, A. M., Kayode, O. I., & Yusuf, D. O. A. (2019). Genetic diversity studies for morphological traits in pearl millet (Pennisetum glaucum L.) landrace of Northern Nigeria. GSC Biological and Pharmaceutical Sciences, 7(2), 60–70. [Google Scholar] [Crossref]

2. Abubakar, S. M., Mohammed, S., Lewu, F. L., Danjuma, M. N., Adetunji, A. T., & Kioko, J. I. (2021). Pearl millet (Pennisetum glaucum [L. R. Rr.]) varietal loss and its potential impact on smallholder farmers in northern Nigeria: A review. Journal of Techno-Social, 12(2), 1–11. [Google Scholar] [Crossref]

3. Adeoti, K., Djedatin, G., Ewedje, E., Beule, T., Santoni, S., Rival, A., & Jaligot, E. (2017). Assessment of genetic diversity among cultivated pearl millet (Pennisetum glaucum, Poaceae) accessions from Benin, West Africa. African Journal of Biotechnology, 16(15), 782–790. [Google Scholar] [Crossref]

4. Ajeigbe, H. A., Angarawai, I. I., Inuwa, A. H., Akinseye, F. M., & AbdulAzeez, T. (2020). Hand book on improved pearl millet production practices in North Eastern Nigeria. [Google Scholar] [Crossref]

5. Anderson, J. A., Churchill, G. A., Autrique, J. E., Tanksley, S. D., & Sorrells, M. E. (1993). Optimizing parental selection for genetic linkage maps. Genome, 36, 181–186. [Google Scholar] [Crossref]

6. Bashir, E. M. A., Abdelbagi, M. A., Adam, M. A., Albrecht, E. M., Heiko, K. P., & Haussmann, B. I. G. (2014). Characterization of Sudanese pearl millet germplasm for agro-morphological traits and grain nutritional values. Plant Genetic Resources, 12(1), 35–47. [Google Scholar] [Crossref]

7. Choi, Y., Kim, K., Lee, S., Oh, S., & Lee, M. (2018). Development of a core collection based on EST-SSR markers and phenotypic traits in foxtail millet [Setaria italica (L.) P. beauv.]. Journal of Crop Science and Biotechnology, 21(4), 395–405. [Google Scholar] [Crossref]

8. Danjuma, M. N., & Abubakar, S. M. (2017). Conservation status of vegetation in the dryland of North-western Nigeria. International Journal of Agriculture, Forestry and Fisheries, 5(2), 18–24. [Google Scholar] [Crossref]

9. Danjuma, M. N., Muhammad, S., & Karkarna, M. Z. (2018). Participation in agroforestry system in North-western Nigeria. Journal of Environmental Sciences and Technology, 2(2), 257–265. [Google Scholar] [Crossref]

10. Deu, M., Sagnard, F., Chantereau, J., Calatayud, C., Vigouroux, Y., Pham, J. L., Mariac, C., Kapran, I., Mamadou, A., Gérard, B., Ndjeunga, J., & Bezançon, G. (2010). Spatio-temporal dynamics of genetic diversity in Sorghum bicolor in Niger. Theoretical and Applied Genetics, 120, 1301–1313. [Google Scholar] [Crossref]

11. Doyle, J. J., & Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13–15. [Google Scholar] [Crossref]

12. Govindaraj, M., Selvi, B., Prabhu, D. A., & Rajarathinam, S. (2009). Genetic diversity analysis of pearl millet (Pennisetum glaucum [L.] R. Br.) accessions using molecular markers. African Journal of Biotechnology, 8(22), 6046–6052. [Google Scholar] [Crossref]

13. Hu, S., Zhou, Y., Zhang, L., Zhu, X., Li, L., Luo, L., Liu, G., & Zhou, Q. (2009). Correlation and quantitative trait loci analyses of total chlorophyll content and photosynthetic rate of rice (Oryza sativa) under water stress and well-watered conditions. Journal of Integrative Plant Biology, 51(9), 879–888. [Google Scholar] [Crossref]

14. Jika, A. K. N., Dussert, Y., Raimond, C., Garine, E., Luxereau, A., Takvorian, N., Djermakoye, R. S., Adam, T., & Robert, T. (2017). Unexpected pattern of pearl millet genetic diversity among ethno-linguistic groups in the Lake Chad Basin. Heredity, 118(1), 491–502. [Google Scholar] [Crossref]

15. Jukanti, A. K., Laxmipathi Gowda, C. L., Rai, K. N., Manga, V. K., & Bhatt, R. K. (2016). Crops that feed the world 11. Pearl millet (Pennisetum glaucum L.): An important source of food security, nutrition and health in the arid and semi-arid tropics. Food Security, 8(2), 307–329. https://doi.org/10.1007/s12571-016-0557-y [Google Scholar] [Crossref]

16. Kapila, R. K., Yadav, R. S., Plaha, P., Rai, K. N., Yadav, O. P., Hash, C. T., & Howarth, C. J. (2007). Genetic diversity among pearl millet maintainers using microsatellite markers. Plant Breeding, 126(7), 234–246. [Google Scholar] [Crossref]

17. Kirouani, A., Babay, E., Ouldkiar, R., Belhadi, B., Souilah, R., Boukhalfoun, L., ... & de Tunisie, A. (2025). Geographical differentiation and genetic diversity of Algerian pearl millet assessed with microsatellites. Revista de la Facultad de Agronomía 42(4): e254256 ISSN 2477-9407 DOI: https://doi.org/10.47280/RevFacAgron(LUZ).v42.n4.XIII [Google Scholar] [Crossref]

18. Kumar, A., Pramanik, J., Jangra, A., Prajapati, B., Kumar, S., & Mehra, R. (2025). Nourishment beyond grains: unveiling the multifaceted contributions of millets to United Nations Sustainable Development Goals. Zeitschrift für Naturforschung C, 80(5-6), 151-162. [Google Scholar] [Crossref]

19. Kumar, S., Hash, C. T., Singh, G., Basava, R. K., & Srivastava, R. K. (2020). Identification of polymorphic SSR markers in elite genotypes of pearl millet and diversity analysis. Ecological Genetics and Genomics, 14, 100051. [Google Scholar] [Crossref]

20. Lin, H.-S., Chiang, C.-Y., Chang, S.-B., Liou, G.-I., & Kuo, C.-S. (2012). Genetic diversity in the foxtail millet (Setaria italica) germplasm as determined by agronomic traits and microsatellite markers. Australian Journal of Crop Science, 6(2), 342–349. [Google Scholar] [Crossref]

21. Meena, S. L., Bana, R. S., Kaur, R., Nirmal, R., Singh, D., Godara, S., ... & Serawat, R. (2025). Long-term moisture conservation and nutrient management improves yield stability and resource use efficiency in pearl millet (Pennisetum glaucum)—mustard (Brassica juncea) system under rainfed ecosystem. Frontiers in Sustainable Food Systems, 9, 1597025. [Google Scholar] [Crossref]

22. Muhammad, S. (2018). Pearl millet characteristics in Futchimiram, North-eastern Yobe state, Nigeria. Journal of Pure and Applied Sciences, 4(1), 340–349. [Google Scholar] [Crossref]

23. Vanisha, S. N., Dhaduk, J. J., Sareen, N., Shahu, T., & Desai, R. (2011). Potential functional implications of pearl millet (Pennisetum glaucum) in health and disease. Journal of Applied Pharmaceutical Science, 1(10), 62–67. [Google Scholar] [Crossref]

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