Synergistic Antibacterial, in-Vitro Anticancer and Molecular Docking Assessment of Carrot, Date Palm and Tiger Nut Juices

This study evaluated the synergistic antibacterial, in-vitro cytotoxic/anti-cancer assessment of carrot (Daucus carrota L., (C), Date palm (Phoenix dactylifera) (D) and Tiger nut (Cyperus esculentus) (T), blends, highlighting their immunostimulatory potentials. Blends were prepared in single, binary and ternary combinations in varying ratios, including 1:1, 2:1, 1:2 and 1:1:1 formulations. Qualitative and quantitative phytochemical analyses showed the presence of flavonoids, phenols, alkaloids, tannins, saponins and terpenoids in varying concentrations across the blends. Antibacterial activity assessed using Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) revealed that the ternary blend (CDT 1:1:1) exhibited the highest anti-microbial efficacy effect on selected microorganisms – Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus pyogenes. Cytotoxicity evaluation using C50 values indicated dose-dependent activity across the three samples. Combination Index (CI) analysis revealed synergistic interactions in most binary and ternary formulations with the CDT (1:1:1) blends showing the strongest synergistic effect. Fifty(50) Wister rats were randomly distributed into ten(10) groups (n=5) with positive and negative controls, treatment groups, and administered graded doses of the blends using carrageenan model. Results showed a dose-dependent response pattern. Statistical analysis was performed using one-way Analysis of Variance (ANOVA), with significance set at p < 0.05. In conclusion the blends possess significant phytochemical constituents, notable antimicrobial and immunostimulatory potential particularly in ternary ratios (CDT 1:1:1). These findings highlight their potential therapeutic relevance. However, further studies are recommended using advanced molecular and standard cancer cell lines.

Synergistics, Antibacterial, Anticancer, Molecular, Docking.

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