Effect of Wheat Bran Incorporation on Physico-Chemical and Microbiological Properties of Buffalo Meat Nuggets during Refrigeration Storage
Authors
Dept.of Livestock Products Technology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, RAJUVAS, Bikaner, Rajasthan (334001) (India)
Dept.of Livestock Products Technology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, RAJUVAS, Bikaner, Rajasthan (334001) (India)
Dept.of Home Science, CBRG, Govt. Girls College, Sriganganagar-335001 (India)
Article Information
DOI: 10.51584/IJRIAS.2026.11070174
Subject Category: Microbiology
Volume/Issue: 11/7 | Page No: 2403-2416
Publication Timeline
Submitted: 2026-08-01
Accepted: 2026-08-06
Published: 2026-08-18
Abstract
This experiment was conducted during January–December, 2024 at Department of Livestock Products Technology, College of Veterinary and Animal Science, RAJUVAS – Bikaner (34001), Rajasthan, India to study the effect of wheat bran on shelf life and physico - chemical properties of buffalo meat nuggets at refrigeration temperature. This study examined the impact of wheat bran incorporation on microbiological parameters such as total plate count (TPC), yeast and mold count (YMC), Coliform count, Staphylococcus aureus count and Clostridium botulinum count of buffalo meat nuggets during refrigeration storage at 4°C .Development of processed meat products involves the incorporation of non-meat ingredients or additives for enhancing the quality attributes, sensory profile, and shelf life. Nuggets were one of the most acceptable value-added commented emulsion-based products that were either baked, steam-cooked or consumed after frying. Total plate count was significantly (p<0.05) decreased in buffalo meat nuggets containing wheat bran and remained less than control during entire storage period. As wheat bran had strong antioxidant properties and was a significant source of bioactive phenolic compound. In contrast, yeast and mold count had not exhibited growth until 21 days of storage but at 28th day, significant (p<0.05) increase in yeast and mold count found in both control and treatment but less increase found in wheat bran treated group as compare to control. This showed the antifungal activity of wheat bran. Pathogenic Escherichia coli, Staphylococcus aureus, and Clostridium botulinum were not detected in both control and treated nuggets during the entire storage period due to hygienic handling.
Keywords
Buffalo meat nugget; Wheat Bran; Physico-Chemical properties; Antioxidant; shelf life
Downloads
References
1. Abbas, O.N., Majeed, M.R., 2024. Antimicrobial and Antibiofilm Activity of Local Wheat Bran Extract and Bacteriocin Combination Against Gastrointestinal Pathogens. Baghdad Science Journal().https://doi.org/10.21123/bsj.2024.10306. [Google Scholar] [Crossref]
2. Abozed, S.S., El-Kalyoubi, M,Abdelrashid, A., Salama, M.F., 2014. Total phenolic contents and antioxidant activities of various solvent extracts from whole wheat and bran. Annals of Agricultural Sciences 59(1), 63–67. [Google Scholar] [Crossref]
3. Ahmad, S.R., Sharma, B.D., Irshad, A., Kumar, R.R., Malav, O.P., Talukder, S., 2021. Effect of aerobic storage conditions on the quality of functional restructured buffalo meat fillets enriched with natural sources of dietary fibers and antioxidant components. Journal of Food Processing and Preservation 45(1), e15072. [Google Scholar] [Crossref]
4. Anson, N.M., Hemery, Y.M., Bast, A., Haenen, G.R., 2012. Optimizing the bioactive potential of wheat bran by processing. Food and function 3(4), 362–375. [Google Scholar] [Crossref]
5. Anonymous, 1984. Compendium of methods for microbiological examination of foods, (2ndEdn.).In: Speck,M.L.,(Ed.),American Public Health AssociationWashington, D.C.Available from : https://www.indianjournals.com/ijor.aspx?target=ijor:ijfv&volume=5&issue=2&article=024&type=pdfAccessed on: 23rd January 2025. [Google Scholar] [Crossref]
6. Anonymous, 2001. Contagem de Clostridium Perfringens Bacteriological Analytical Manual. In: Chapter 16, Food and Drug Administration, Clostridium Perfringens.Available from :https://www.scirp.org/journal/paperinformation?paperid=44057. Accessed on: 23rd January 2025. [Google Scholar] [Crossref]
7. Anonymous, 2024. Government of India. Ministry of Agriculture and Farmers Welfare, Department of Animal Husbandry, New Delhi. Available from:https://dahd.gov.in/sites/default/files/202501/FinalBAHS2024Book14012025.pdf. Accessed on 23rd January 2025. [Google Scholar] [Crossref]
8. Anonymous, 1984. Compendium of Methods for Microbiological Examination of Foods (2nd edn.). In:Speck, M.L.,(Ed.), American Public Health Association, Washington, D.C. [Google Scholar] [Crossref]
9. Badr, H.M., Mahmoud, K.A., 2011. Antioxidant activity of carrot juice in gamma irradiated beef sausage during refrigerated and frozen storage. Food Chemistry127(3), 1119–1130. [Google Scholar] [Crossref]
10. Balandran-Quintans, R.R.,J.N., Mercado-Ruiz., A.M, MendozaWilson., 2015. Wheat bran proteins: A review of their uses and potential. Food Reviews International 31(3), 279–93. doi: 10.1080/ 87559129.2015.1015137. [Google Scholar] [Crossref]
11. Bansil, P.C., 2003. Demand for food grains. In: Dev, S.M., Kanan, K.P. and Ramachandran, M., (Eds.), Towards a Food Secure India: Issues and policies, Institute for Human Development, New Delhi, p.5. [Google Scholar] [Crossref]
12. Barnes, H.J., 2003.Isolamento de Clostridium Perfringens. In: Diseases of Poultry, Iowa State University Press, Ames, 797–859. [Google Scholar] [Crossref]
13. Bhat, A.A., Kumar, A., Dua, S., 2016. Storage quality and oxidative stability attributes of almond (Prunusdulcis) fortified chevon nuggets. Journal of Animal Research 6(4), 747–753. [Google Scholar] [Crossref]
14. Das, A.K., Nanda, P.K., Madane, P., Biswas, S., Das, A., Zhang, W., Lorenzo, J.M., 2020. A comprehensive review on antioxidant dietary fibre enriched meat-based functional foods. Trends in Food Science and Technology 99, 323–336. [Google Scholar] [Crossref]
15. Devatkal, S.K., Thorat, P., Manjunatha, M., 2014. Effect of vacuum packaging and pomegranate peel extract on quality aspect of ground meat nuggets. Journal of Food Science and Technology 51(10), 2685–2691. [Google Scholar] [Crossref]
16. Di Lena, G., Vivanti, V., Quaglia, G.B., 1997. Amino acid composition of wheat milling by‐products after bioconversion by edible fungi mycelia. Food/Nahrung 41(5), 285–288. [Google Scholar] [Crossref]
17. Di Stasio, L., Brugiapaglia, A., 2021. Current knowledge on river buffalo meat: A critical analysis. Animals 11(7), 2111. [Google Scholar] [Crossref]
18. Ehsanbakhsh, M., Sadeghi, A., Raeisi, M., Ebrahimi, M., Kashaninejad, M., 2017. Isolation, Molecular Identification and Evaluation of Antifungal Effect of Dominant Lactic Acid Bacteria Isolated from Wheat Bran and Rice Bran Sourdoughs. Research and Innovation in Food Science and Technology 6(3), 245–260. [Google Scholar] [Crossref]
19. Fang, Z., Lin, P., Ha, M., Warner, R.D., 2019. Effects of incorporation of sugarcane fibre on the physicochemical and sensory properties of chicken sausage. International Journal of Food Science and Technology 54(4), 1036–1044. [Google Scholar] [Crossref]
20. Ferysiuk, K., Wójciak, K.M., 2020. Reduction of nitrite in meat products through the application of various plant-based ingredients. Antioxidants 9(8), 711. [Google Scholar] [Crossref]
21. Gunenc, A., Alswiti, C., Hosseinian, F., 2017. Wheat bran dietary fiber: promising source of prebiotics with antioxidant potential. Journal of Food Research 6(2),1. [Google Scholar] [Crossref]
22. Holmer, S.F., McKeith, R.O., Boler, D.D., Dilger, A.C., Eggert, J.M., Petry, D.B.,Killefer, J., 2009. The effect of pH on shelf-life of pork during aging and simulated retail display. Meat Science 82(1), 86–93. [Google Scholar] [Crossref]
23. Hromádková, Z., Paulsen, B.S., Polovka, M., Košťálová, Z., Ebringerová, A., 2013. Structural features of two heteroxylan polysaccharide fractions from wheat bran with anti-complementary and antioxidant activities. Carbohydrate polymers93(1), 22–30. [Google Scholar] [Crossref]
24. Jin, S.K., Choi, J.S., Kim, G.D., 2021. Effect of porcine plasma hydrolysate on physicochemical, antioxidant, and antimicrobial properties of emulsion-type pork sausage during cold storage. Meat science 171,108293. [Google Scholar] [Crossref]
25. Jung, J.T., Lee, J.K., Choi, Y.S., Lee, J.H., Choi, J.S., Choi, Y.I.,Chung, Y.K., 2018. Effect of rice bran and wheat fibers on microbiological and physicochemical properties of fermented sausages during ripening and storage. Korean journal for food science of animal resources 38(2), 302. [Google Scholar] [Crossref]
26. Kamal, S.B., Kumar, A., Tanwar, T., 2017. Physico-chemical, proximate, sensory and storage quality attributes analysis of papaversomniferum (poppy) fortified chevon nuggets. Journal of Applied and Natural Science 9(1), 114–120. [Google Scholar] [Crossref]
27. Kaur, S., Kumar, S., Bhat, Z.F., Kumar, A., 2015. Effect of pomegranate seed powder, grape seed extract and tomato powder on the quality characteristics of chicken nuggets. Nutrition and Food Science 45(4), 583–594. [Google Scholar] [Crossref]
28. Kumar, L., Bhat, Z.F., Kumar, S., 2016a. Effect of nisin on the quality characteristics of fiber-enriched chicken harrisa. Journal of Meat Science 11(2), 57–63. [Google Scholar] [Crossref]
29. Kumar, P., Chatli, M.K., Mehta, N., Malav, O.P., Verma, A.K., Kumar, D., 2016b. Quality attributes and storage stability of chicken meat biscuits incorporated with wheat and oat bran. Journal of Food Quality 39(6), 649–657. [Google Scholar] [Crossref]
30. Laddomada, B., Caretto, S,Mita, G., 2015. Wheat bran phenolic acids: Bioavailability and stability in whole wheat-based foods. Molecules 20(9), 15666–15685. [Google Scholar] [Crossref]
31. Li, C., Stump, M., Wu, W., Li, Y., 2023. Exploring the chemical composition, antioxidant potential, and bread quality effects of the nutritional powerhouse: Wheat bran–A mini-review. Journal of Agriculture and Food Research 100898. [Google Scholar] [Crossref]
32. Meena, P., 2021. Studies on alpha-linolenic acid (Flaxseed) enriched chevon nuggets incorporated with green coffee (Coffeaarebica) and grape seed extract (Vitisvinifera).MVSc. Thesis, Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan. [Google Scholar] [Crossref]
33. Nahid, M.Z.A., Habib, M., Mollah, M.B.R., Hashem, M.A., Azad, M.A.K.,Ali, M.S., 2024. Development of dietary fiber enriched chicken nugget using wheat bran. Meat Research 4(1),1–6. [Google Scholar] [Crossref]
34. Onipe, O.O., Ramashia, S.E., Jideani, A.I., 2021. Wheat bran modifications for enhanced nutrition and functionality in selected food products. Molecules26(13), 3918. [Google Scholar] [Crossref]
35. Ravani, A., Sharma, H.P., 2022. Functional Foods.In: Meat based functional foods, 235–287. [Google Scholar] [Crossref]
36. Ribeiro, J.S., Santos, M.J.M.C., Silva, L.K.R., Pereira, L.C.L., Santos, I.A., da Silva Lannes, S.C., da Silva, M.V., 2019. Natural antioxidants used in meat products: A brief review. Meat science 148, 181–188. [Google Scholar] [Crossref]
37. Rindhe, S.N., Chatli, M.K., Wagh, R.V., Kumar, P., Malav, O.P., Mehta, N., 2018. Development and quality of fiber enriched functional spent hen nuggets incorporated with hydrated wheat bran. International Journal of Current Microbiology and Applied Sciences 7(12), 3331–3345. [Google Scholar] [Crossref]
38. Shanaullah, M., Habib, M., Hashem, M. A., Azad, M. A. K. and Ali, M. S., 2024. Development of dietary fiber enriched chicken sausage using wheat bran. Meat Research 4(2), 1–7. [Google Scholar] [Crossref]
39. Shukla, M. K.,2017. Effect of chicken skin blend containing wheat bran on quality characteristics of cooked sausages. M.V.Sc. Thesis, GB Pant University of Agriculture and Technology, Pantnagar, Uttarakhand. [Google Scholar] [Crossref]
40. Snedecor, G.K., Cochran, W.G., 1989. Statistical Methods (8thEdn.).Lowa state university press, Ames, USA, Lowa-50010, pp [Google Scholar] [Crossref]
41. Soren, N. M., Biswas, A. K., 2020. Methods for nutritional quality analysis of meat. In: Meat quality analysis, Academic Press, 21–36. [Google Scholar] [Crossref]
42. Talukder, S. and Sharma, D. P., 2010. Development of dietary fiber rich chicken meat patties using wheat and oat bran. Journal of food science and technology 47, 224–229. [Google Scholar] [Crossref]
43. Trout, E.S., Hunt, M.C., Johnson, D.E., Claus, J.R., Kastner, C.L.,Kropt, D.H., 1992. Characteristics of low fat ground beef containing texture modifying ingredients. Journal of Food Science 57(1) 19–24. [Google Scholar] [Crossref]
44. Verma, B., Hucl, P., Chibbar, R.N., 2008. Phenolic content and antioxidant properties of bran in 51 wheat cultivars. Cereal Chemistry 85(4), 544–549. [Google Scholar] [Crossref]
45. Xiong, Y., Zhang, P., Warner, R.D., Shen, S., Johnson, S.,Fang, Z., 2020. Comprehensive profiling of phenolic compounds by HPLC-DAD-ESI-QTOF-MS/MS to reveal their location and form of presence in different sorghum grain genotypes. Food Research International 137, 109671. [Google Scholar] [Crossref]
46. Xiong, Y., Zhang, P., Warner, R.D., Hossain, M.N., Leonard, W., Fang, Z., 2022. Effect of sorghum bran incorporation on the physicochemical and microbial properties of beef sausage during cold storage. Food Control132, 108544. [Google Scholar] [Crossref]
47. Yadav, S., Pathera, A.K., Islam, R.U., Malik, A.K., Sharma, D.P., 2017. Effect of wheat bran and dried carrot pomace addition on quality characteristics of chicken sausage. Asian-Australasian journal of animal sciences 31(5), 729. [Google Scholar] [Crossref]
48. Yilmaz, I., 2005. Physicochemical and sensory characteristics of low fat meat balls with added wheat bran. Journal of Food Engineering 69,369–373. [Google Scholar] [Crossref]
49. Zhang, A., de Koning, L., Shannon, H.S., Anand, S.S., 2010. A systematic review of the evidence supporting a causal link between dietary factors and coronary heart disease. Archives of Internal Medicine169, 659–669. [Google Scholar] [Crossref]
50. Zhang, H., Zhang, M., Zheng, X., Xu, X., Zheng, J., Hu, Y., Liang, Y., 2024. Solid-State Fermentation of Wheat Bran with Clostridium butyricum: Impact on Microstructure, Nutrient Release, Antioxidant Capacity, and Alleviation of Ulcerative Colitis in Mice. Antioxidants 13(10), 1259. [Google Scholar] [Crossref]
51. Zou, Z., Purnawan, M.A., Wang, Y., Ismail, B.B., Zhang, X., Yang, Z., Guo, M., 2025. A novel antimicrobial peptide WBp-1 from wheat bran: Purification, characterization and antibacterial potential against Listeria monocytogenes. Food Chemistry 463, 141261. [Google Scholar] [Crossref]
52. Zou, Z., Wang, M., Wang, Z., Aluko, R.E., He, R.,2020. Antihypertensive and antioxidant activities of enzymatic wheat bran protein hydrolysates. Journal of Food Biochemistry 44(1), e13090. [Google Scholar] [Crossref]
Metrics
Views & Downloads
Similar Articles
- Quantitative Assessment of Serum C - Reactive Protein Levels among Smokers and Non Smokers
- Extraction and Characterization of Eco-Friendly Hair Dye from Agricultural Waste: A Study on Coconut Husk
- Intermittent Preventive Treatment among Pregnant Women Attending a Tertiary Healthcare Facility in Jos, North Central Nigeria: Adherence Patterns and Preliminary Assessment of Associated Factors
- Assessment Of Degradative Potentials Of Bacteria Isolated From Palm Oil-Polluted Site Using Spectrophotometric Method
- An Analysis of Machine and Deep Learning Insights on the Use of Artificial Intelligence in Oral and Maxillofacial Pathology