Exploring Electrical Activity of a Bacterial and Yeast Cell for Potential Production of Power in a Tropical Setting

This study investigated and compared the individual and combined electricity-generating potential of Saccharomyces cerevisiae and Lactobacillus bulgaricus in double-chambered microbial fuel cells (MFCs) in Makurdi, Benue State, Nigeria. The experimental setup consisted of two identical MFC units run under similar conditions with a fixed one thousand Ω external resistance. Electrical parameters including open circuit voltage (OCV), voltage across resistor, current output, conductivity, temperature, and pH were monitored over 7 days. Results showed that OCV values ranged from 3.6 mV to 686.9 mV in the S. cerevisiae system and from 76.8 mV to 732.3 mV in the Lactobacillus system. Current output increased progressively in both systems with values ranging from 0.0 mA to 0.6 mA for S. cerevisiae, and 0.0 mA to 0.7 mA for L. bulgaricus. The voltage across the external resistor varied between 2.3 mV and 658.5 mV in the yeast-based MFC and 44.4 mV and 702.9 mV in the Lactobacillus system. Anode conductivity increased during operation, ranging from 34.9 µS/cm to 580.7 µS/cm in the yeast system and 815.1 µS/cm to 1866.0 µS/cm in the Lactobacillus system, showing improved ionic mobility and substrate degradation. Anode pH values varied between 5.6 and 4.2 in the yeast MFC and 3.7 and 4.9 in the Lactobacillus MFC due to microbial metabolic activities. Temperature remained within the mesophilic range of 29.6°C to 32.6°C in the anode chamber, and 21.6°C to 30.0°C in the cathode chamber, providing suitable conditions for microbial growth and bioelectricity production. The yeast-based MFC produced higher peak power output of 0.4935 mW, while the Lactobacillus system produced 0.4333 mW. Overall, the findings show that both microorganisms can generate electricity in MFCs, although the yeast system produced slightly higher peak electrical output while the Lactobacillus system exhibited more sustained performance.

Bioelectricity, Lactobacillus, Microbial Fuel Cells, Renewable Power, S. cerevisiae

1. Aghababaie, M., Ghadiri, M., and Rahimnejad, M. (2021). Enhancing efficiency of microbial fuel cells: Role of operational factors and materials. Renewable Energy, 16(9), 170–182 [Google Scholar] [Crossref]

2. Bond, D. R., and Lovley, D. R. (2022). Electricity production by Geobacter sulfurreducens attached to electrodes. Applied and Environmental Microbiology, 69(3), 1548–1555 [Google Scholar] [Crossref]

3. Chaudhuri, S. K., and Lovley, D. R. (2003). Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells. Nature Biotechnology, 21(10), 1229 - 1232. https://doi.org/10.1038/nbt867 [Google Scholar] [Crossref]

4. Choi, S. (2019). Microbial fuel cells: Challenges and future prospects. Environmental Engineering Science, 36(2), 105 – 119 [Google Scholar] [Crossref]

5. Du, Z., Li, H., and Gu, T. (2016). A state-of-the-art review on microbial fuel cells: A promising technology for wastewater treatment and bioenergy. Biotechnology Advances, 25(5), 464 – 482 [Google Scholar] [Crossref]

6. Feng, Y., Yang, Q., Wang, X., and Logan, B. E. (2019). Influence of environmental parameters on bacterial electricity generation in MFCs. Bioresource Technology, 104(1), 34 – 39 [Google Scholar] [Crossref]

7. He, Z., Wagner, N., and Minteer, S. D. (2020). Microbial fuel cells: From fundamentals to applications. Energy and Environmental Science, 13(4), 1137 – 1161 [Google Scholar] [Crossref]

8. Huang, L., Chai, X., and Zheng, P. (2022). Decentralized electricity production in remote areas using microbial fuel cells. Journal of Cleaner Production, 35(2), 131517. [Google Scholar] [Crossref]

9. International Energy Agency (2022). Global energy review 2022. Retrieved from https://www.iea.org/reports/global-energy-review-2022 [Google Scholar] [Crossref]

10. Kurniawan, T. A., Lo, W., and Chan, G. Y. S. (2021). Optimizing pH in microbial fuel cells for better electricity generation. Environmental Science and Pollution Research, 28(4), 4367 – 4377 [Google Scholar] [Crossref]

11. Lie, T. J., Kim, B. C., and Smith, J. A. (2018). Electrochemical communication in bacterial communities. Journal of Bacteriology, 200(5), e00525-17 [Google Scholar] [Crossref]

12. Logan, B. E., Hamelers, B., Rozendal, R., Schröder, U., Keller, J., Freguia, S., Aelterman, P., Verstraete, W., and Rabaey, K. (2006). Microbial fuel cells: Methodology and technology. Environmental Science and Technology, 40(17), 5181 – 5192 [Google Scholar] [Crossref]

13. Logan, B. E., Rossi, R., and Kim, K. Y. (2019). Electrochemical perspectives on microbial fuel cells and waste-to-energy conversion. Nature Reviews Microbiology, 17(5), 307 – 319 [Google Scholar] [Crossref]

14. Niessen, J., Schröder, U., Rosenbaum, M., and Scholz, F. (2019). Flavins as electron shuttles in microbial fuel cells. Electrochemistry Communications, 7(6), 107 -111 [Google Scholar] [Crossref]

15. Pandya, M., Shah, T., and Singh, R. (2024). Microbial fuel cells for sustainable electricity: Current status and future prospects. Renewable Energy, 21(2): 123456 [Google Scholar] [Crossref]

16. Pandya, R. S., Kaur, T., Bhattacharya, R., Bose, D., and Saraf, D. (2024). Harnessing microorganisms for bioenergy with Microbial Fuel Cells: Powering the future. Water-Energy Nexus, 7(6) 1-12 [Google Scholar] [Crossref]

17. Pant, D., Singh, A., Van Bogaert, G., Irving Olsen, S., and Vanbroekhoven, K. (2019). Biological fuel cells: Mechanism and factors affecting performance. Bioresource Technology Reports, 7(2): 100228 [Google Scholar] [Crossref]

18. Prescott, L. M., Harley, J. P., and Klein, D. A. (2002). Microbiology (5th ed.). McGraw-Hill. [Google Scholar] [Crossref]

19. Rabaey, K., Rodríguez, J., Blackall, L. L., Keller, J., Gross, P., Batstone, D., and Verstraete, W. (2020). Microbial ecology meets electrochemistry: Electricity-driven and driving communities. ISME Journal, 3(7); 745 – 758 [Google Scholar] [Crossref]

20. Ringeisen, B. R., Henderson, E., and Logan, B. E. (2019). Emerging applications of microbial fuel cells in bioenergy and bioremediation. Current Opinion in Biotechnology, 22(3): 1 – 6 [Google Scholar] [Crossref]

21. Santoro, C., Arbizzani, C., Erable, B., and Ieropoulos, I. (2017). Microbial fuel cells: From fundamentals to applications. Journal of Power Sources, 35(6): 225 – 244 [Google Scholar] [Crossref]

22. Sun, J., Yang, X., and Yuan, Y. (2018). Microbial fuel cells for renewable electricity generation and wastewater treatment. Environmental Progress and Sustainable Energy, 37(4), 1430 – 1437 [Google Scholar] [Crossref]

23. Tender, L. M., Reimers, C. E., Stecher, H. A., Holmes, D. E., Bond, D. R., and Lovley, D. R. (2021). Harnessing microbially generated power on the seafloor. Nature Biotechnology, 20(8); 821 – 825 [Google Scholar] [Crossref]

24. Zhou, Minghua, Chi, Meiyan, Luo, Junfeng, He, Hui, and Jin, Tao (2019). Recent advances in microbial fuel cells: A review. Renewable and Sustainable Energy Reviews, 10(5); 204 – 212 [Google Scholar] [Crossref]

25. Zou, C., Zhao, Q., Zhang, G., and Xiong, B. (2022). Energy revolution: From a fossil energy era to a new energy era. Natural Gas Industry, 3(1), 1-11 [Google Scholar] [Crossref]

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