Identification of Oil Producing Yeast and Characterization of Its Oil

Single cell oil which is also known as microbial oil are produced by oleaginous microorganisms which have ability to accumulate more than 20% lipid per dry biomass, having potential applications in food, feed and pharmaceutical industry. Most of the lipids in this oil are triglycerides characterized with long chain fatty acids similar to that of vegetable oils. This study investigated the growth and identification of oil producing yeasts. Furthermore, the characterizations of the oil fatty acids composition were analyzed using gas chromatography mass spectroscopy (GSMS). The oleaginous yeast Rhodotorula mucilaginosa RIT389 and Pichia kudriavzevii 10526S from different sources were used for the investigation the lipid accumulation was 30.8% and 38.5% of the dry cell weight (DCW) respectively.
From the analysis, saturated fatty acids (SFA) have highest percentage i.e. 60.82% and 78.60% respectively in the two microbial oil studied in which palmitic acid, stearic acid, myristic and lauric acid are the major SFA. Others include pentadecnoic acid and shorter chains fatty acids such as acetic acid, propanoic acid and butanoic acid. Also, monounsaturated fatty acids (MUFA) contain 32.32% and 19.03% respectively and polyunsaturated fatty acids (PUFA) recorded the lowest percentage of 6.86% and 2.37% respectively in the two oil studied. These findings underscore the potential of oleaginous yeasts as alternative lipid sources. The compositional similarity of the microbial oils to conventional edible oils such as soybean and palm oil supports their feasibility for sustainable industrial applications.

oleaginous yeast, Single-cell oil, Lipid accumulation, MUFA

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