Preparation, Evaluation and Comparative Study of Herbal Lozenges from Plectranthus Amboinicus Leaf Extract and Essential Oil

Plectranthus amboinicus is an herb species ordinarily well-known as Panikoorka of the family of Lamiaceae. These plant species are recognized in every part of India and it is a medicinal plant which is utilised in folkloric medicines for diseases like Flu, bronchitis and epilepsy. This study aims to formulate and evaluate herbal lozenges from Plectranthus amboinicus leaf extract & essential oil and to compare the antibacterial activity of it. Fresh leaves of Plectranthus amboinicus were collected, authenticated and subjected to cold maceration to obtain hydroalcoholic extract.
The preliminary phytochemical screening indicated the presence of a diverse range of bioactive compounds, including flavonoids, alkaloids, tannins, terpenoids, glycosides, phenolic compounds, and phytosterols. In this study lozenges are prepared with Plectranthus amboinicus leaf extract and its essential oil.
The prepared lozenges underwent organoleptic evaluation, assessing shape, texture, odor, color, taste, and type, as well as physicochemical characterization, including disintegration test, dissolution test, hardness, diameter, and weight variation. An in vitro study was conducted to evaluate the antimicrobial activity of the prepared herbal lozenges against Escherichia coli (E. coli), a Gram-negative bacterium.
The antimicrobial activity of Plectranthus amboinicus lozenges was evaluated, and the results showed that the lozenges exhibited a higher inhibition zone compared to the extract. This enhanced antimicrobial activity is attributed to the synergistic effects of the various ingredients present in the lozenges, including peppermint oil, glycerin, jaggery, and sugar, which possess inherent antibacterial properties that complement the bioactive compounds present in Plectranthus amboinicus.

Plectranthus amboinicus, Antibacterial activity, Lozenges

1. Punet Kumar et.al, Pharmacognostical, Pharmacological Studies and Traditional uses of Plectranthus Ambonicus: A Review, Research Journal of Pharmacognosy and Phytochemistry. 11(4): October- December, 2019 ;245 [Google Scholar] [Crossref]

2. R. Muthukumarana, R.M. Dharmadasa, Pharmacognostical investigation of Plectranthus hadiensis (Forssk.) Schweinf. ex Sprenger. and Plectranthus amboinicus (Lour.) Spreng, World Journal of Agricultural Research, 2014, Vol. 2, No. 5, 240-246 [Google Scholar] [Crossref]

3. Greetha Arumugam et.al, Review Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance, Molecules 2016, 21, 369, 30 January 2016; Accepted: 8 March 2016; Published: 30 March 2016. [Google Scholar] [Crossref]

4. Kamlendra Kumar Mishra et al, Formulation and evaluation of herbal lozenges, Journal of Drug Delivery and Therapeutics.2017;7(7)87 [Google Scholar] [Crossref]

5. Punet Kumar et.al, Pharmacognostical, Pharmacological Studies and Traditional uses of Plectranthus Ambonicus: A Review, Research Journal of Pharmacognosy and Phytochemistry. 11(4): October- December, 2019 ;245 [Google Scholar] [Crossref]

6. V.P Kamboj, HERBAL MEDICINE, General Article, Current science.10 january 2000;78(3) [Google Scholar] [Crossref]

7. Poojitha .M, Saravana Kumar, S.V. Satyanarayana, Pharmacognostic study data of Indian medicinal plants, International Journal of Pharmaceutical Research. 2020; 12 (3) [Google Scholar] [Crossref]

8. Kamlendra Kumar Mishra et al, Formulation and evaluation of herbal lozenges, Journal of Drug Delivery and Therapeutics.2017;7(7)87 [Google Scholar] [Crossref]

9. Kshirsagar Sanjeevani et.al, Formulation & Evaluation Of Herbal Lozenges For Flu And Cold, 2023 IJNRD | Volume 8, Issue 5 May 2023 | ISSN: 2456-4184 | IJNRD.ORG,page no.205. [Google Scholar] [Crossref]