Sub-Lethal Toxicity of Paraquat Dichloride (200g/L) on Biochemical Properties of Clarias Gariepinus Juveniles

This study investigates the sub-lethal effects of varying concentrations of Paraquat dichloride on biochemical parameters and mortality rates of juvenile Clarias gariepinus over a 28-day exposure period. A controlled laboratory experiment was conducted with five treatment groups (T1 to T5) of juvenile C. gariepinus, each comprising nine fish. The control group (T1) received no Paraquat, while T2 to T5 were exposed to increasing concentrations (0.034 to 0.134 ml/L) of Paraquat dichloride (200 g/L). Mortality rates increased significantly with higher Paraquat concentrations, peaking at 66.67% in T5. Results also revealed the biochemical disruptions caused by Paraquat exposure indicated increased glucose levels, liver dysfunction and altered cholesterol metabolism in C. gariepinus, suggesting physiological stress and toxicity of juvenile C. gariepinus leading to elevated mortality and compromised health. This study emphasized the critical role of biochemical assessments in evaluating health impacts of pollutants on fish

Sub-lethal toxicity, C. gariepinus, Paraquat

1. Adebayo, O. T., Afolabi, A. A., & Olaniyan, A. B. (2020). Effects of agricultural runoff on the hematology of fish in Lagos State, Nigeria. African Journal of Aquatic Science, 45(2), 123-130. https://doi.org/10.2989/16085914.2020.1764882 [Google Scholar] [Crossref]

2. Ameen, S. M., Emeash, H. M., & Saad, M. M. (2018). Biochemical effects of paraquat herbicide in Clarias gariepinus. Journal of Fish and Aquatic Science, 13(2), 31-40. [Google Scholar] [Crossref]

3. Baker, S. J., Larese-Casanova, P., & Sturgeon, R. E. (2019). Ecotoxicological assessment of pesticide effects on aquatic ecosystems. Environmental Science & Technology, 53(9), 5256-5265. https://doi.org/10.1021/acs.est.8b05864 [Google Scholar] [Crossref]

4. Blažek, J., Vejrazka, J., & Volf, J. (2020). Oxidative stress in fish: A review. Aquatic Toxicology, 219, 105395. https://doi.org/10.1016/j.aquatox.2019.105395 [Google Scholar] [Crossref]

5. Boudou, A., & Ribeyre, F. (2006). Effects of herbicides and pesticides on aquatic organisms: A review of the literature. Environmental Pollution, 144(2), 429-442. https://doi.org/10.1016/j.envpol.2006.03.015 [Google Scholar] [Crossref]

6. Carpenter, S. R., Caraco, N. F., Correll, D. L., Howarth, R. W., Sharpley, A. N., & Smith, V. H. (2021). Nonpoint pollution of surface waters with phosphorus and nitrogen. Ecological Applications, 20(3), 975-988. https://doi.org/10.1890/03-1022 [Google Scholar] [Crossref]

7. Davis, J. R., Wootton, R. J., & Lestari, P. (2018). Metabolic responses of fish to environmental stressors. Fish Physiology and Biochemistry, 44(5), 1217-1230. https://doi.org/10.1007/s10695-018-0545-6 [Google Scholar] [Crossref]

8. FAO. (2022). The State of World Fisheries and Aquaculture 2022: Toward Blue Transformation. Food and Agriculture Organization. https://www.fao.org/3/cc0461en/cc0461en.pdf [Google Scholar] [Crossref]

9. García, E., López, A., & González, A. (2022). Biochemical responses of fish to chemical stressors: Implications for health and environmental monitoring. Environmental Monitoring and Assessment, 194(8), 516. https://doi.org/10.1007/s10661-022-10424-x [Google Scholar] [Crossref]

10. González, M., Ramírez, G., & López, M. (2020). Toxic effects of paraquat on fish: A review. Environmental Science and Pollution Research, 27(15), 18433-18445. https://doi.org/10.1007/s11356-020-08751-7 [Google Scholar] [Crossref]

11. Ibrahim, M. K., & Dawood, M. A. (2018). Toxicity of paraquat and its effects on the liver and other tissues of fish. Environmental Toxicology and Pharmacology, 57, 89-97. https://doi.org/10.1016/j.etap.2017.12.011 [Google Scholar] [Crossref]

12. Khan, F. R., Rahman, M. M., & Ahmed, F. (2021). Reactive oxygen species and antioxidant defense mechanisms in fish. Aquatic Toxicology, 236, 105846. https://doi.org/10.1016/j.aquatox.2021.105846 [Google Scholar] [Crossref]

13. Little, E. E., Arnot, J. A., & Peterman, R. M. (2003). Effects of pollution on fish behavior: Implications for fish health and management. Aquatic Toxicology, 63(1), 1-9. https://doi.org/10.1016/S0166-445X(02)00088-9 [Google Scholar] [Crossref]

14. Mansour, S. A., Sidky, M. M., & Khedher, N. B. (2021). Sub-lethal effects of agricultural pesticides on fish physiology and biochemistry: A review. Fish Physiology and Biochemistry, 47(1), 1-18. https://doi.org/10.1007/s10695-020-00858-7 [Google Scholar] [Crossref]

15. Matsuo, A., Yoshida, T., & Sakakibara, H. (2015). Effects of herbicides on biochemical parameters in fish. Environmental Pollution, 206, 18-26. https://doi.org/10.1016/j.envpol.2015.06.018 [Google Scholar] [Crossref]

16. Moniruzzaman, M., Saha, N., & Ali, M. (2020). Sub-lethal effects of pesticides on fish and aquatic ecosystems: A review. Environmental Toxicology and Pharmacology, 76, 103349. https://doi.org/10.1016/j.etap.2020.103349 [Google Scholar] [Crossref]

17. Olufemi, O. R., Ojo, J. O., & Olagunju, A. I. (2021). Hematological changes in Clarias gariepinus exposed to environmental pollutants: Implications for aquaculture. Environmental Science and Pollution Research, 28(15), 18678-18686. https://doi.org/10.1007/s11356-021-13363-1 [Google Scholar] [Crossref]

18. Sánchez, M. C., Pacheco, M., & Fernández, A. (2020). Pesticides and their effects on aquatic organisms: A review. Environmental Pollution, 258, 113868. https://doi.org/10.1016/j.envpol.2019.113868 [Google Scholar] [Crossref]

19. Van Dyk, J. C., Dalu, T., & Smit, H. (2020). The effects of chemical pollutants on aquatic ecosystems: A global perspective. Environmental Pollution, 259, 113808. https://doi.org/10.1016/j.envpol.2019.113808 [Google Scholar] [Crossref]

20. Zhou, Z., Zhang, J., & Wang, D. (2011). Effects of Paraquat on fish health and biochemistry: A review. Chemosphere, 85(5), 756-764. https://doi.org/10.1016/j.chemosphere.2011.06.039 [Google Scholar] [Crossref]

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