Influence of Certain Parameters on Laboratory Analyses and Verification of Preservatives in Natural Apple and Peach Juices

Authors

Diellon Salihaj

Faculty of Biotechnical Sciences, University “St. Kliment Ohridski” – Bitola (North Macedonia)

Prof. Dr. Juliana Tomovska

Faculty of Biotechnical Sciences, University “St. Kliment Ohridski” – Bitola (North Macedonia)

Article Information

DOI: 10.51244/IJRSI.2026.1308000239

Subject Category: Medical Laboratory Science

Volume/Issue: 13/8 | Page No: 3847-3856

Publication Timeline

Submitted: 2026-09-03

Accepted: 2026-09-08

Published: 2026-09-18

Abstract

This study evaluates the influence of selected technological and quality parameters on the physicochemical, microbiological, and organoleptic characteristics of natural apple and peach juices, together with the verification of selected preservatives and artificial sweeteners. Apple and peach purees were subjected to initial quality control upon reception, including pH, °Brix, and organoleptic assessment. The technological process followed modern production standards. Juices were diluted with treated water based on °Brix values, and acidity was adjusted using citric acid or lemon puree. Peach juice received natural flavor to compensate for aroma loss, while apple juice was processed without additional flavoring. After homogenization, juices were pasteurized at 85–95 °C for 15–30 seconds to inactivate microorganisms and enzymes. Packaging materials were sterilized, and filling was performed aseptically. Final tunnel pasteurization (90–95 °C for approximately 30 minutes) ensured microbiological stability and extended shelf life. Samples were collected at three stages: after raw material reception, after formulation and homogenization, and after final packaging. Laboratory analyses included organoleptic evaluation, microbiological testing, physicochemical measurements, and HPLC analysis. HPLC was used to verify the presence or absence of potassium sorbate (E202), sodium benzoate (E211), and selected artificial sweeteners. The chromatographic analysis showed that E202, E211, and the analyzed artificial sweeteners were not detected in the juice samples. The results also indicated that refrigerated storage provided greater physicochemical stability compared with ambient storage. These findings demonstrate the importance of technological processing, appropriate storage conditions, and analytical monitoring in maintaining juice quality and verifying compliance with food safety and labeling requirements.

Keywords

Apple juice, Peach juice, Preservatives, Physicochemical analysis, HPLC

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