Association of ABO Blood Groups with Bleeding Time and Clotting Time among MBBS Students: A Cross-Sectional Observational Study

Blood Group in human is completely & unchangeable identity. Blood group antigens are genetically determined and are inherited as Mendelian determinants. The relationship between ABO blood group and are associate with various diseases for example gastric cancer periodontal disease and Venus thrombosis. Some author found that epistaxis is commonly seen in people with O blood due to there is a low expression of Von Willebrand factor (vWf) causing a relative bleeding tendency. Haemostasias refers to the natural physiological mechanism that prevents and stops bleeding, thereby maintaining vascular integrity. The objective of the study was to assess the relationship between bleeding time and clotting time among various blood groups and to identify any gender difference among the same.
Our study included 300 MBBS students (180 males and 120 females) in the age group of 17–21 years. The blood grouping was carried out with the standard antisera, and bleeding time and clotting time were estimated by Duke method and capillary glass tube method respectively. Finally, bleeding time and clotting time of different blood groups were compared and statistical analysis was carried out.
Results: Blood group O was the most common blood group in both genders followed by B, A, and AB. Bleeding time in relation with blood group was found to be statistically insignificant. Also, clotting time in relation within blood group was found to be statistically non-significant. Similarly, gender-wise bleeding time in relation with blood group was found to be statistically insignificant. Also, clotting time in relation within blood group was found to be statistically non-significant and clotting time was also statistically insignificant.

Blood Group, Bleeding Time (BT), Clotting Time (CT), von Willebrand factor (vWf)

1. Poonam G Kohli, Harleen Kaur2*, and Seema Maini. Relationship of Bleeding Time and Clotting Time with Blood Groups. RJPBCS March - April 2014 5(2) Page No. 1780 -1783). [Google Scholar] [Crossref]

2. Dean L. Blood Groups and Red Cell Antigens. Bethseda(MD): National Centre for Biotechnology Information (U.S.); 2005. [Google Scholar] [Crossref]

3. Manjeet Kaur, Arvinder Singh,Roopam Bassi, Deepinder Kaur. Blood group distribution and its relationship with bleeding time and clotting time. National Journal of Physiology, Pharmacy and Pharmacology 2015 Vol 5 Issue 3, 253 – 257. [Google Scholar] [Crossref]

4. Hanrui Zhang, Ciaran J. Mooney and Muredach P. Reilly. ABO Blood Groups and Cardiovascular Diseases. International Journal of Vascular Medicine 2012; Article ID 641917: 1-11. [Google Scholar] [Crossref]

5. Wiggins KL, Smith NL, Glaeer N L, Rosendal FR, Heckbert SR, Psaty BM, Rice KM, Lumley T. ABO genotype and risk of thrombotic events and hemorrhagic stroke. Journal of thrombosis and haemostasis 2008; 7:263-269. [Google Scholar] [Crossref]

6. Reddy, Daniel M, Bright E, Board SR and Moir AA. Is there an association between blood group O and epistaxis? J. Laryngol. Oto! 2008; 122(4): 366- 368. [Google Scholar] [Crossref]

7. Thenmozhi, S., Neelambikai, N. and Aruna P. (2013) Comparison of Bleeding Time and Clotting Time in different ABO Blood Groups. National Journal of Physiology, 1, 19-24. [Google Scholar] [Crossref]

8. Franchini M, Franco Capra, Giovanni Targher, Martina Montagnana, Giuseppe Lippi. Relationship between ABO blood group and von Willebrand factor levels: from biology to clinical implications. ThrombJ.2007;5:14. [Google Scholar] [Crossref]

9. Mourant AE. Blood Relations: Blood Groups and Anthropology. Oxford: Oxford University Press, 1983.pp1–146 [Google Scholar] [Crossref]

10. Qureshi MA, Bhatti R. Frequency of ABO blood groups among the diabetes mellitus type 2 patients. J Coll Physicians Surg Pak. 2003;13:453–5. [Google Scholar] [Crossref]

11. Ruggeri ZM..Structure of von Willebrand factor and its function in platelet adhesion and thrombus formation. Best Pract Res Clin Haematol.2001;14:257–9. [Google Scholar] [Crossref]

12. Gill JC, Endres-Brooks J, Bauer PJ, Marks WJ Jr, Montgomery RR. The effect of ABO blood group on the diagnosis of von Willebrand disease. Blood.1987;69(6):1691–5. [Google Scholar] [Crossref]

13. Daniel M, Jaberoo MC, Stead RE, Reddy VM, Moir AA. Is admission for epistaxis more common in Caucasian than in Asian people? Preliminary study. Clin Otolaryngol 2006; 31: 386–89. [Google Scholar] [Crossref]

14. Ghai CL. A Textbook of Practical Physiology. 9th ed. 68-78,79-88. [Google Scholar] [Crossref]

15. Mahapatra B, Mishra N. Comparison of bleeding time and clotting time in different blood groups. American J Infectious Dis. 2009; 5(2):113–15. [Google Scholar] [Crossref]

16. Sasekala M, Saikumar P. Relationship between bleeding time and clotting time among gender difference and varying blood groups in UG medical students. IOSR J Dental Medical Sci. 2013;10(6):40–3. [Google Scholar] [Crossref]

17. Patil SV, Gaikwad PB, Vaidya SR, Patil US, Kittad SD. To study the blood group distribution and its relationship with bleeding and clotting time in dental students. Asian J Medical Pharmaceutical Sci. 2013;1(1):1–4. [Google Scholar] [Crossref]

18. Roy B Banerjee, Sathian B, Mondal M, Saha CG. Blood group distribution and its relationship with bleeding time and clotting time: a medical school based observational study among Nepali. Indian and Sri Lankan students. Nepal J Epidemiol. 2011;1(4):135–140. [Google Scholar] [Crossref]

19. Abhishekh B, Mayadevi S, Meena D, Usha KC. Distribution of ABO and Rhesus-D blood groups in and around Thiruvananthapuram. Kerala Med J. 2011;1:28–9. [Google Scholar] [Crossref]

20. Jenkins PV, O’Donnell JS. ABO blood group determines plasma von Willebrand factor levels: a biologic function after all? Transfusion. 2006;46(10):1836–1844. [Google Scholar] [Crossref]

21. Adhikari P, Pramanik T, Pokhael R, Khanal S. Nepal Med Coll J 2008;10(4): 264-265. [Google Scholar] [Crossref]

• A Comparative Study of Adjustment Level, Parental Income and Academic Achievement of Adolescent Girls
• Increase in STIs among Adolescents in Masvingo District
• A Correlation between SDG 4 (Quality Education) and Emotional Disposition of Teacher Educators in Hyderabad District
• Ear Piercing Behaviour and Self-Esteem among Male University Students in Nigeria
• A Study of Religiosity and Psychological Well-Being