Antibiotic Susceptibility Profile of Pseudomonas Aeruginosa and Other Bacteria from Fish in Okwan Obolo Estuary, Nigeria
Authors
Department of Microbiology, University of Uyo (Nigeria)
Department of Microbiology, University of Uyo (Nigeria)
Department of Microbiology, University of Uyo (Nigeria)
Department of Microbiology, University of Uyo (Nigeria)
Article Information
DOI: 10.51244/IJRSI.2026.13014003
Subject Category: Microbiology
Volume/Issue: 13/14 | Page No: 24-32
Publication Timeline
Submitted: 2025-12-23
Accepted: 2025-12-29
Published: 2026-01-29
Abstract
This study was carried out to determine the antibiotics susceptibility profile of Pseudomonas aeruginosa and other bacteria from fish harvested in Okwan Obolo Estuary. Using an ice-cold pack, 1g of fish gills, muscles and intestine were used for the microbiological analysis. Antibiotic sensitivity tests were done on each isolates. The Total heterotrophic bacterial count was 1.02 x 106 CFU/g, 8.6 x 105 CFU/g and 3.0 x 105 CFU/g in gills, intestine and muscles respectively. Pseudomonas aeruginosa count in Intestine, Gills and Muscles were 5.4 x 105 CFU/g, 2.1 x 105 CFU/g and 1.1 x 105 CFU/g respectively. Total coliform count of 7.5 x 105 CFU/g was obtained from intestine, while 3.9 x 105CFU/g and 1.9 x 105CFU/g were recorded from gills and muscles respectively. The research further showed that other bacteria isolated from the fish were Bacillus spp., Proteus spp., Shigella spp., Klebsiella spp., Enterobacter spp., Salmonella spp. and Escherichia coli. The result shows that the most widely distributed bacterial isolates were P. aeruginosa and Salmonella spp. with 100% (3) occurrence, while the least distributed was Proteus spp. and Klebsiella spp. with 33.3% (1) occurrence. The most prevalent bacteria isolates were Pseudomonas aeruginosa and Salmonella spp. with 18.75% percentage prevalence, while the least prevalent bacteria isolates were Proteus spp. and Klebsiella spp with 6.25% prevalence. The antibiotics used were Gentamycin, Ciproflaxacin, Tetracycline, Erythromycin, Streptomycin and Neomycin. The isolates showed high resistant to Tetracycline, Neomycin and Erythromycin with 7mm zone of inhibition, and most isolates were sensitive to Gentamycin, Ciprofloxacin and Streptomycin with highest zone of inhibition of 21mm, 25mm and 24mm respectively. The isolation of multiple antibiotics resistant strains of Pseudomonas aeruginosa from fish in Okwan Obolo Estuary is an epidemiological concern, as the isolate is capable of causing infection in human.
Keywords
Antibiotic, Susceptibility, Profile, Pseudomonas Aeruginosa, Bacteria, Fish
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