Antioxidant Activity, Lipid Peroxidation Suppression and Preliminary Toxicity Assessment of Persicaria Minor (Kesum) Aqueous Extract

Persicaria minor is a widely consumed Southeast Asian herb with emerging pharmacological relevance; however, comprehensive evaluation integrating antioxidant efficacy and safety profiling remains limited. This study systematically investigated the in vitro antioxidant capacity, in vitro lipid peroxidation modulation, and preliminary toxicity profile of the aqueous leaf extract of P. minor. Antioxidant activity was assessed using DPPH radical scavenging and ferric reducing antioxidant power (FRAP) assays, while total phenolic content (TPC) was quantified via the Folin–Ciocalteu method. Oxidative stress attenuation was evaluated by measuring malondialdehyde (MDA) levels, and preliminary safety was determined using the Brine Shrimp Lethality Test (BSLT). The extract demonstrated total phenolic content reaching approximately 500 mg GAE/g at 1 mg/mL. DPPH radical scavenging activity ranged from approximately 73–94% inhibition across concentrations (0.046875–6 mg/mL), approaching the activity of ascorbic acid (~98%) at higher doses. FRAP analysis revealed consistent ferric reducing capacity, with values around 159.5–159.7 µmol Fe²⁺ equivalents across tested concentrations. In the lipid peroxidation model, MDA levels were reduced from approximately 4.6–4.8 nmol/mg protein in the negative control to about 1.8–1.9 nmol/mg protein in the treated group, representing roughly 55–60% inhibition. Brine shrimp survival rates remained above 80% across concentrations (0.097–100 mg/mL), with no LC₅₀ detected within the tested range, indicating low acute toxicity. Collectively, these findings demonstrate that P. minor aqueous extract possesses potent antioxidant activity, effectively mitigates lipid peroxidation, and exhibits minimal preliminary toxicity, highlighting its potential as a safe natural antioxidant candidate for further pharmacological and toxicological investigations.

Persicaria minor; aqueous leaf extract; antioxidant activity; DPPH

1. Altanam SY, Darwish N, Bakillah A. Exploring the Interplay of Antioxidants, Inflammation, and Oxidative Stress: Mechanisms, Therapeutic Potential, and Clinical Implications. Diseases. 2025 Sep 22;13(9):309. doi: 10.3390/diseases13090309. PMID: 41002745; PMCID: PMC12469104. [Google Scholar] [Crossref]

2. Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014;2014:360438. doi: 10.1155/2014/360438. Epub 2014 May 8. PMID: 24999379; PMCID: PMC4066722. [Google Scholar] [Crossref]

3. Barrera G, Pizzimenti S, Daga M, Dianzani C, Arcaro A, Cetrangolo GP, Giordano G, Cucci MA, Graf M, Gentile F. Lipid Peroxidation-Derived Aldehydes, 4-Hydroxynonenal and Malondialdehyde in Aging-Related Disorders. Antioxidants (Basel). 2018 Jul 30;7(8):102. doi: 10.3390/antiox7080102. PMID: 30061536; PMCID: PMC6115986. [Google Scholar] [Crossref]

4. Bas TG. Dietary Polyphenols (Flavonoids) Derived from Plants for Use in Therapeutic Health: Antioxidant Performance, ROS, Molecular Mechanisms, and Bioavailability Limitations. Int J Mol Sci. 2026 Jan 30;27(3):1404. doi: 10.3390/ijms27031404. PMID: 41683824; PMCID: PMC12898187. [Google Scholar] [Crossref]

5. Cordiano R, Di Gioacchino M, Mangifesta R, Panzera C, Gangemi S, Minciullo PL. Malondialdehyde as a Potential Oxidative Stress Marker for Allergy-Oriented Diseases: An Update. Molecules. 2023 Aug 9;28(16):5979. doi: 10.3390/molecules28165979. PMID: 37630231; PMCID: PMC10457993. [Google Scholar] [Crossref]

6. Gulcin İ. Antioxidants: a comprehensive review. Arch Toxicol. 2025 May;99(5):1893-1997. doi: 10.1007/s00204-025-03997-2. Epub 2025 Apr 15. PMID: 40232392; PMCID: PMC12085410. [Google Scholar] [Crossref]

7. Juan CA, Pérez de la Lastra JM, Plou FJ, Pérez-Lebeña E. The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. Int J Mol Sci. 2021 Apr 28;22(9):4642. doi: 10.3390/ijms22094642. PMID: 33924958; PMCID: PMC8125527. [Google Scholar] [Crossref]

8. Kruk J, Aboul-Enein BH, Duchnik E, Marchlewicz M. Antioxidative properties of phenolic compounds and their effect on oxidative stress induced by severe physical exercise. J Physiol Sci. 2022 Aug 5;72(1):19. doi: 10.1186/s12576-022-00845-1. PMID: 35931969; PMCID: PMC10717775. [Google Scholar] [Crossref]

9. Mirfat, A.H.S., Mohd. Effendi, M.N., Norma, H., Muhammad Faris, M.R., Muhammad Faidhi, T., Ainon, D.Z. & Hanim, A. (2024) ‘Evaluation of phytochemical, antioxidant and antimicrobial properties of different accessions of Persicaria minor (kesum)’, Food Research, 8(Supp. 4), pp. 57–64. https://doi.org/10.26656/fr.2017.8(S4).6 [Google Scholar] [Crossref]

10. Molole GJ, Gure A, Abdissa N. Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin. BMC Chem. 2022 Jun 25;16(1):48. doi: 10.1186/s13065-022-00841-x. PMID: 35752844; PMCID: PMC9233799. [Google Scholar] [Crossref]

11. Nhamussua RL, Mabiki FP, Mwakalesi AJ, McGaw LJ. Screening anticancer activity by Brine shrimp lethality test of extracts of Annona stenophylla (Engl. & Diels), Strophanthus petersianus (Klotzsch) and Synadenium glaucescens (Pax). PLoS One. 2026 Jan 2;21(1):e0336636. doi: 10.1371/journal.pone.0336636. PMID: 41481610; PMCID: PMC12758728. [Google Scholar] [Crossref]

12. Payne AC, Mazzer A, Clarkson GJ, Taylor G. Antioxidant assays - consistent findings from FRAP and ORAC reveal a negative impact of organic cultivation on antioxidant potential in spinach but not watercress or rocket leaves. Food Sci Nutr. 2013 Nov;1(6):439-44. doi: 10.1002/fsn3.71. Epub 2013 Oct 16. PMID: 24804054; PMCID: PMC3951540. [Google Scholar] [Crossref]

13. Pohan, D.J., Marantuan, R.S. & Djojosaputro, M. (2023) ‘Toxicity test of strong drug using the BSLT (Brine Shrimp Lethality Test) method’, International Journal of Health Sciences and Research, 13(2), pp. 203. https://doi.org/10.52403/ijhsr.20230228 [Google Scholar] [Crossref]

14. Roy A, Khan A, Ahmad I, Alghamdi S, Rajab BS, Babalghith AO, Alshahrani MY, Islam S, Islam MR. Flavonoids a Bioactive Compound from Medicinal Plants and Its Therapeutic Applications. Biomed Res Int. 2022 Jun 6;2022:5445291. doi: 10.1155/2022/5445291. PMID: 35707379; PMCID: PMC9192232. [Google Scholar] [Crossref]

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