Trace Elements in Oral Biology and Dental Practice: Molecular Mechanism and Clinical Applications

In oral biology, trace elements are crucial because they affect the host immune system, microbial ecology, salivary function, and the structural integrity of tooth tissues. Fluoride, zinc, copper, selenium, iron, manganese, and other elements are involved in the mineralization of dentin, the development of enamel, antioxidant defense systems, and enzymatic control. These micronutrients influence the onset and course of dental caries, periodontal diseases, and disorders of the oral mucosa by modifying signaling pathways, gene expression, collagen formation, and oxidative stress responses at the molecular level. While fluoride encourages remineralization and inhibits demineralization by producing fluorapatite, zinc and copper boost antibacterial activity and matrix metalloproteinase regulation. Manganese and selenium protect oral tissues from damage from reactive oxygen species because they are cofactors for antioxidant enzymes. Both an excess and a deficit of trace elements can disrupt oral homeostasis, which can lead to reduced enamel, delayed wound healing, altered salivary composition, and increased susceptibility to infection. Trace elements are used in clinical practice for a variety of prophylactic and therapeutic purposes, including dental implants, bioactive restorative materials, fluoride products, and adjunctive periodontal therapy. Recent research shows their potential in nanotechnology-based drug delivery systems and regenerative dentistry. This review covers the most recent studies on the molecular mechanisms and clinical applications of trace elements in dentistry, with an emphasis on their dual role in maintaining dental health and causing disease when out of balance. Understanding these linkages is the foundation for developing targeted therapeutic and preventive approaches in modern dentistry.

Trace elements, Dentistry, Oral biology

1. Anuradha CD, Devi CS. Serum protein, ascorbic acid, iron copper tissue collagen in oral sub mucous fibrosis. Indian J Med Research 1993; 98:147-51. [Google Scholar] [Crossref]

2. Anuradha CD, Devi CS. Studies on Hematological profile and trace elements in oral sub mucous fibrosis. J ClinBiochemNutr 1995;12: 9-17. [Google Scholar] [Crossref]

3. Arnold Brossi. The alkaloids. Chemistry and physiology, Vol 32, 1950 Richard Helmuth Fred Manske, Henry Lavergne Holmes. Academic press. [Google Scholar] [Crossref]

4. Chatterje M.N. Text book of Biochemistry . 8th edition. 2012. Page 8. Jaypee Brothers medical publishers (p) ltd. New Delhi, Panamacity, London. [Google Scholar] [Crossref]

5. Vasudevan DM, Sree Kumara S, KannanVidyanathan. Text book of Biochemistry for medical students. 2ndedition, 1998, page no 379. Jaypee Brother, medical publishers(P) Ltd, New Delhi. [Google Scholar] [Crossref]

6. Fauci A, Braunwald E, Isselbacr K, Wilson J, Martin J, Kasper D et al., Harrison’s principle of internal medicine, 14th edition, 1997, Page 1027, Megraw – Hill Publisher. [Google Scholar] [Crossref]

7. Merlan and Ernest. Metal and their compounds in environment occurrence – analysis cubiological relevance, Ist Edition 1991,CRC publication. [Google Scholar] [Crossref]

8. Rooban T, Saraswathi TR, Antony George , Elizabeth Joshua, Ranganathan K. Cytological study of copper in oral sub mucous fibrosis. Ind J Dent Res 2004;15(4):129-134. [Google Scholar] [Crossref]

9. Trivedy C, Baldwin D, Warnakulasuriya S, Johnson NW, Peters T. Copper content in areca catechu products and oral sub mucous fibrosis. Lancet 1997; 349 : 144-47. [Google Scholar] [Crossref]

10. Trivedy CR, Warnakulasuriya KA, Peters TJ, Senkus R, Hazarey VK, Johnson NW. Raised tissue copper levels in oral sub mucous fibrosis. J Oral Pathol Med. 2000; 29(6): 241-248. [Google Scholar] [Crossref]

11. Rajendran R, Vasudevan DN, Vijayakumar.T. Serum levels of iron, copper, proteins in oral sub mucous fibrosis. Anna. Dent 1990; 49 (2): 23-25. [Google Scholar] [Crossref]

12. Suzukik, Oyama R, Hayashi E, Arakawa Y, Nippon – Rinsho . Liver diseases and essential trace elements. 1996; 54(1): 85 – 92. [Google Scholar] [Crossref]

13. Kobune M, Koto J, Kohgo A, Nitish Y. Liver cirrhosis in pulmonary hematochromasis as Wilson’s diseases. Nippon Rinsho 1994; 52(1): 209-14. [Google Scholar] [Crossref]

14. Arnold Brossi. The alkaloids. Chemistry and physiology, Vol 32, Richard Helmuth Fred Manske, Henry Lavergne Holmes. Academic press. 1950. [Google Scholar] [Crossref]

15. Vergheese I, Suganthan CK, Balasubramanian G, Vijay kumar T. Serum copper and Zinc levels in oral sub mucous fibrosis. Oncology 1987; 44(4): 224-7. [Google Scholar] [Crossref]

16. Thomas I.T, Evans E.J. The effect of cobart chromium molybdenum powder on collagen formation by fibroblast in vitro.Bimaterials 1986; 7(4): 301-4. [Google Scholar] [Crossref]

17. Bihr B, Deyl Z, Lener J, Kuara J, Adam M. Investigation on the reaction of Molybdenum with collagen vivo. Int J Pept Protein Res. 1977; 10(3): 190-6. [Google Scholar] [Crossref]

18. Arbogast S and Ferretro A. Selenoproteins andprotectine against oxidative stress: selenoprotein N as a noval player at the cross roads of redox signaling and caliumhomeostastsis. Anti OxidRedoxxsingal 2010; 12: 893-904. [Google Scholar] [Crossref]

19. Back MA, Shi , Morris VC and Levander OA. Rapid genomic evolution of a non virulentcoxsackic virus B3 in selenium deficient mice resuts in selection of identification virulent isolates. Nat Med 1995;1:433-36. [Google Scholar] [Crossref]

20. Burke Grffney A, Callister ME and Nakamura H. Thioredoxin : friend or foe in human diases? Trends PharmacolSci 2005;26:398-404. [Google Scholar] [Crossref]

21. Haldimann m, alt A, Blanc A, Blondeau K. iodine content of food groups. J Food Comp anal 2005;18:461-71. [Google Scholar] [Crossref]

22. Phillips DI. Iodine milk and the elimination of endemic goiter in Britain: the story of an accidental public health triumph. J Epidemiol Community Health 1997;51:391-93. [Google Scholar] [Crossref]

23. Frieden E. New perspectives on the essential trace elements. J ChemEdu 1985;62:917-23. [Google Scholar] [Crossref]

24. Kienlen J. Deficiences in trace elements during parenteral alimentation. AnnAnesthesiolFr 1977;18:1019-34. [Google Scholar] [Crossref]

25. Reitnerova E, Amarasiriwardena D, Kopcakova M, Barnes RM. Determination of some trace elements in human tooth enamel. Fresenius J Anal Chem 2000;367(8):748-54. [Google Scholar] [Crossref]

26. Neto JB. The essential role in inc in growth. Nutr Res 1995;15:335-58. [Google Scholar] [Crossref]

27. Navia JM. Effect of minerals on dental caries. AdvChem 1970;94:123-60. [Google Scholar] [Crossref]

28. Pindborg JJ, Sirsat SM. Oral sub muocus fibrosis. Oral Surg Oral Med Oral Pathol 1966;22:764-79. [Google Scholar] [Crossref]

29. Tilakaratne WM, Kilnikowski MF, Saku T, Peters TJ, Warnakulasuriya S. Oral Sub mucous fibrosis : Review on aetiology and pathogenesis. Oral Oncol 2006;42:561-8. [Google Scholar] [Crossref]

30. Trivedy C, Warnakulasuriya KAAS, Hazarey VK, Tavassoli M, Sommer P, Johnson NW. The upregulation of lysyloxidase in oral sub mucous fibrosis and squamous cell carcinoma. JOPM 1999;28(6):246-251. [Google Scholar] [Crossref]

31. Peryrol S, Raccurt M, Gerard F, Gleyzal C etal., Lysyl oxidase gene expression into the stromal reaction to insitu and invasive ductal breast carcinoma. Am J Pathol 1997; 150(2): 497-507. [Google Scholar] [Crossref]

32. Trivedy C, Baldwin D, Warnakulasuriya S, Johnson N, Peters T. copper content in areca catechu (betel nut) products and oral sub mucous fibrosis . Lancet 1997;349:1447-49. [Google Scholar] [Crossref]

33. Romero Chapman etal., Purification, Properties and Influence of Dietary copper on accumulation and functional activity of lysyl oxidase in rat skin. Biochem. J. 1991; 275:657-662. [Google Scholar] [Crossref]

34. Sokol R.J. etal., Anti oxidant defenses in metal induced liver damage. Seminar on liver diseases. 1996; Feb 16(1): 39-46. [Google Scholar] [Crossref]

35. Shakya S, Ongole R, Sumanth KN. Copper content of various constituents of betel quid. Indian J Dental Res 2009;20:516-7. [Google Scholar] [Crossref]

36. Freeman BA, Crapo JD. Biology of diseases: Free radicals and tissue injury. Lab Invest 1982;47:412-26. [Google Scholar] [Crossref]

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