Development and Evaluation of a Herbal Spray for its Repellent and Insecticidal Effects Containing Wedelia Chinensis, Aloe Vera and Lavender Oil

Authors

Sathi Paul M

Raghu college of Pharmacy, Visakhapatnam, Andhra Pradesh (India)

D Sri Harshini

Raghu college of Pharmacy, Visakhapatnam, Andhra Pradesh (India)

M Jaya Krupa

Raghu college of Pharmacy, Visakhapatnam, Andhra Pradesh (India)

Prathiba Kumari

Raghu college of Pharmacy, Visakhapatnam, Andhra Pradesh (India)

B Tanooja

Raghu college of Pharmacy, Visakhapatnam, Andhra Pradesh (India)

Durga Prasad

Raghu college of Pharmacy, Visakhapatnam, Andhra Pradesh (India)

Article Information

DOI: 10.51244/IJRSI.2026.1308000069

Subject Category: Pharmaceutics

Volume/Issue: 13/8 | Page No: 842-851

Publication Timeline

Submitted: 2026-08-03

Accepted: 2026-08-08

Published: 2026-09-03

Abstract

Wedelia chinensis extract, Aloe vera gel, and lavender oil as a safe and eco-friendly alternative to conventional chemical repellents. Ethanolic extract of Wedelia chinensis was Mosquito-borne diseases such as malaria, dengue, chikungunya, and Zika continue to pose significant public health challenges worldwide. Although synthetic mosquito repellents are effective, their prolonged use is associated with skin irritation, toxicity, environmental concerns, and the development of insect resistance. The present study aimed to formulate and evaluate a herbal mosquito repellent spray containing prepared by maceration and incorporated into the formulation along with Aloe vera gel, lavender oil, glycerine, polysorbate 80, and distilled water. The prepared formulation was evaluated for physicochemical parameters including pH, viscosity, and spreadability, followed by mosquito repellent activity, antimicrobial activity, insecticidal potential, and skin irritation testing. The formulation exhibited a skin-friendly pH of 5.5–6.5, good spreadability, and suitable viscosity for topical application. Mosquito repellent studies demonstrated approximately 85% repellency, indicating effective protection against mosquito bites. The formulation also showed moderate antimicrobial activity and insecticidal potential while producing no signs of skin irritation or allergic reactions during topical application. The synergistic combination of Wedelia chinensis, Aloe vera, and lavender oil provided enhanced repellent efficacy, skin-soothing effects, and improved formulation stability. These findings suggest that the developed herbal mosquito repellent spray is a safe, effective, biodegradable, and environmentally friendly alternative to synthetic mosquito repellents, with promising potential for further development as a commercial herbal product.

Keywords

Wedelia chinensis, Herbal mosquito repellent, Lavender essential oil, Aloe vera, Mosquito repellency, Biodegradable formulation, Vector control

Downloads

References

1. World Health Organization. (2020).Vector-borne diseases and their global burden. WHO Bulletin, 98(2), 79–86.Mowat,Alex P. Year. Liver Disorders in Childhood. Butterworth-Heinemann. [Google Scholar] [Crossref]

2. Surjushe, A., Vasani, R., & Saple, D.G. (2008). Aloe vera: A short review. Indian Journal of Dermatology, 53(4), 163–166. [Google Scholar] [Crossref]

3. Hamman, J.H. (2008). Composition and applications of Aloe vera leaf gel. Molecules, 13(8), 1599–1616. [Google Scholar] [Crossref]

4. Fluhr, J.W., Darlenski, R., & Surber, C. (2008). Glycerol and the skin: holistic approach to its origin and functions. British Journal of Dermatology, 159(1), 23–34. [Google Scholar] [Crossref]

5. Service, M.W. (2012). Medical Entomology for Students (5th ed.). Cambridge University Press. [Google Scholar] [Crossref]

6. Surjushe, A., Vasani, R., & Saple, D.G. (2008). Aloe vera: A short review. Indian Journal of Dermatology, 53(4), 163–166. [Google Scholar] [Crossref]

7. Hamman, J.H. (2008). Composition and applications of Aloe vera leaf gel. Molecules, 13(8), 1599–1616. [Google Scholar] [Crossref]

8. Fluhr, J.W., Darlenski, R., & Surber, C. (2008). Glycerol and the skin: holistic approach to its origin and functions. British Journal of Dermatology, 159(1), 23–34. [Google Scholar] [Crossref]

9. Service, M.W. (2012). Medical Entomology for Students (5th ed.). Cambridge University Press. [Google Scholar] [Crossref]

10. Syed, Z., & Leal, W.S. (2008). Mosquitoes smell and avoid the insect repellent DEET. Proceedings of the National Academy of Sciences, 105(36), 13598–13603. [Google Scholar] [Crossref]

11. WHO Pesticide Evaluation Scheme (WHOPES). (2009). Guidelines for efficacy testing of mosquito repellents for human skin. Geneva: World Health Organization. [Google Scholar] [Crossref]

12. World Health Organization (WHO). (2009). Guidelines for efficacy testing of mosquito repellents for human skin. Geneva: WHO Press. [Google Scholar] [Crossref]

13. Perez, C., Pauli, M., & Bazerque, P. (1990). An antibiotic assay by the agar well diffusion method. Acta Biologiae et Medicinal Experiments, 15, 113–115 [Google Scholar] [Crossref]

14. Luker AM, et al. (2024). A critical review of current laboratory methods used to evaluate mosquito repellents. Frontiers in Insect Science. It describes filter-paper-based excito-repellency testing and methods for calculating repellency. [Google Scholar] [Crossref]

15. Sarma R, Adhikari K, Mahanta S, Khanikor B. Combinations of plant essential oil based terpene compounds as larvicidal and adulticidal agent against Aedes aegypti (Diptera: Culicidae). Scientific Reports. 2019;9:9471. The study used Whatman No. 1 filter paper for the adulticidal assay and evaluated mortality over time. [Google Scholar] [Crossref]

16. Draize, J.H., Woodard, G., & Calvery, H.O. (1944). Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. Journal of Pharmacology and Experimental Therapeutics, 82(3), 377390. [Google Scholar] [Crossref]

17. Pavela, R. (2016). History, presence and perspective of using plant extracts as commercial botanical insecticides. Plant Protection Science, 52(4), 229–241 [Google Scholar] [Crossref]

18. Kennedy MK, et al. Performance of the plant-based repellent TT-4302 against mosquitoes in the laboratory and field and comparative efficacy to 16 mosquito repellents against Aedes aegypti (Diptera: Culicidae). Journal of Medical Entomology. 2014;51(2):392–399. DOI: [Google Scholar] [Crossref]

19. 1603/ME12240. [Google Scholar] [Crossref]

Metrics

Views & Downloads

Similar Articles