Hair Growth Pathway, A Novel Innovative Method for Assessment and Detection of Chronic Stress in Adult Female Wistar Rats.

1. Amina Inova ,Viktoria Birkenges, Daniel S Qiuntena, Monica Beg Ormered (2025).Cortisol and C- reactive protein (CRP) regulation in severe mental disorders. Psychrneuroendocrinology. Volume 172,107272 4. .
2. 5. Anna – karrin lennartsson, Elin Anvidson, Mats –Borjesson , Inigibing-Jonsdotin (2022). DHEA-S 6. production capacity in relation to perceived prolonged stress The International journal on the Biology of stress. Volume 25,Issue/Pages 105-112.
3. Arielo Niraulo, Kristno G withcher, John F, Sheridon, Jonathan P.Godboat (2019).Interleukin-6 9. Induced by social stress promotes a unique transcriptional signature in the monocytes that 10. facilitates anxiety biological psychiatry volume 85,Issue 8.Page 679-689.
4. Cay M, Ucar C, Senol D, Cevirgen F, Ozbag D, Altay Z, Yildiz S.(2018) Effect of increase in cortisol level due to stress in healthy young individuals on dynamic and static balance scores.
5. North Clin Istanb. 2018 May 29;5(4):295-301. doi: 10.14744/nci.2017.42103. PMID: 30859159; PMCID: PMC6371989.
6. Das, A.(2019). Chronic Ongoing stressors and c-reactive protein: A within – Person Study.
7. Journal of Aging and Health. Volume 32. Issue 7-8.
8. Bobko, N.A. (2007)Effect of stress on the cardiovascular system activity in operators of 18. predominantly mental work at different times of the day and the working week. Hum Physiol 33, 302–308. https://doi.org/10.1134/S0362119707030073.
9. Bouillon-Minois JB, Trousselard M, Thivel D, Gordon BA, Schmidt J, Moustafa F, Oris C, Dutheil F. Ghrelin as a Biomarker of Stress: A Systematic Review and Meta-Analysis. (2021).
10. Nutrients. Feb 27;13(3):784. doi: 10.3390/nu13030784. PMID: 33673594; PMCID: PMC7997253.
11. Christine Fahrngruber. Velasquez, Kalina Duszka and Jurgen Konig(2021).The impact of chronic stress and Eating concern on Acylased Ghrelin following Acute Psychological stress in Healthy Men.Stresses1(1),16-29.
12. Eleanor Mclaughan, Julian Barth H (2017). An analysis of the relationship between serum cortisol and serum sodium in routine clinical patients.Practical Laboratory Medicine.
13. Eva MJ peters,Yvonne Muller,Wenke Snaga,Herbert Fliege,Anett Reishaver,Thomas – Schmidt Rose(2017).Hair and stress: A pilot study of hair and cytokine balance alteration in healthy young women under major exam stress. Plos One.10.1371/Journal pone.0175904
14. Golbidi S, Frisbee JC, Laher I(2015).Chronic stress impacts the cardiovascular system: animal models and clinical outcomes. Am J Physiol Heart Circ Physiology .308:1476-1498.
15. Gong S, Miao YL, Jiao GZ, Sun MJ, Li H, Lin J, Luo MJ, Tan JH. (2015).Dynamics and correlation 33. of serum cortisol and corticosterone under different physiological or stressful conditions in mice. PLoS One. 2015 Feb 20;10(2):e0117503. doi: 10.1371/journal.pone.0117503. PMID: 25699675; PMCID: PMC4336318.
16. Hwee-Soo Jeong (2018). The status of hair minerals in chronic fatigue and depressed women.
17. Asian journal of pharmaceutical and clinical research. Vol 11, Issue 11,485-488
18. Jitendia R Dave, Sally M. Anderson, George A Saviolaks, Edward H.Mougey(2000).Chronic sustained stress increases levels of anterior pituitary prolactin mRNA, Pharmacol Biochem Behaviour 67(3):423-31.
19. Kudiela BM, Broderick JE, Kirschbaum C (2003). Compliance with saliva sampling protocols: electronic monitoring reveals invalid cortisol daytime profiles in noncompliant subjects. Psychomatic medicine 65(2):313-9.PMID:12652000.
20. Lauren A Stone, Elia S Harmatz, Ki A Grosens(2020)Ghrelin as a stress hormone; Implications for psychiatric illness. Biological Psychiatry .Volume 88, Issue 7,Page 531-540.
21. Lightman SL, Wiles CC, Henley DE, Wood SA, Wood SA (2008). The significance of glucocorticoid pulsatility. Eur J Pharmacol; 583:255-262.
22. Margheritta sisto and francesc Grans (2024). Immunology of stress: A review Article. J clin Med;13(21):6394.
23. Mahmut Cay, Cihat Ucar, Deniz Senol, Furkan Cevigran (2018). Effects of increase in cortisol 51. level due to stress in healthy young individuals on dynamic and static balance scores.Northern clinics of Istanbul May 29;5(4):295-301.
24. Melissa Dong, Matthew T. McGoldrick, Heather Seid, Laura P. Cohen, Ariana LaRocca, Patrick Pham, S. Justin Thomas, Joseph E. Schwartz, Daichi Shimbo(2022).
25. The stress, salexcretion, and nighttime blood pressure (SABRE) study: Rationale and study design, American Heart 56. Journal Plus: Cardiology Research and Practice, Volume 13,100099, ISSN 2666- 6022,https://doi.org/10.1016/j.ahjo.2022.100099.
26. Nabill Ibrahim, Esam Eweis, Hossam El-Beltagi, Yasmin Abel-Mobby(2012).Effect of lead 59. acetate toxicity on experimental male albino rat. Asian Pacific Journal of tropical Biomedicine ; 2(1):41- 46.
27. Parasuraman S,Ravendran R,Kesavan R(2010)Blood Sample collection in small laboratory animals.J harmacol,87-93.
28. Piruzian, L.A., Protasova, O.V., Maksimova, I.A. (2014).Calcium and phosphorus contents of human body during 105-day isolation. Hum Physiol 40, 822–826. https://doi.org/10.1134/S0362119714070226.
29. Qing H, Desrouleux R, Israni Winger K, Mineur YS, Fogelman N, Zhang C, Radheed S, Palm NW ,Sinta R(2020).Origin and function of stress – Induced IL-6immune models cell.cell.com P1660 Pubmed 372387, volume 182,Issue 6
30. Ren J, Guo J, Zhu S, Wang Q, Gao R, Zhao C, Feng C, Qin C, He Z, Qin C, Wang Z, Zang L.(2021)The Role of Potassium Channels in Chronic Stress-Induced Brain Injury. Biol Pharm Bull. 2021 Feb 1;44(2):169-180. doi: 10.1248/bpb.b20-00504. Epub 2020 Nov 25. PMID: 33239494
31. Samara Levine, Ozgul Muneyeriei (2018). Stress Induced hyperprolactinemia: pathophysiology and clinical Approach. Obstet Gynecol Int.Doi:10.1155/2018/9253083
32. Sanjeevs s. walvekar, Jeevan G. Ambekah, Basavaraj B(2015).Study on serum cortisol and 74. perceived stress scale in the police constables. Journal of clinical and Diagnostic Research Vol.9(2):BCID – BC14.
33. Satoh E, Tada Y, Matsuhisa F.(2011) Chronic stress enhances calcium mobilization and glutamate exocytosis in cerebrocortical synaptosomes from mice. Neurol Res. 2011 Nov;33(9):899-907. doi: 10.1179/1743132811Y.0000000033. PMID: 22080989.
34. Schaafsma FG, Hulsegge G, de Jong MA, Overvliet J, van Rossum EFC, Nieuwenhuijsen K.
35. (2021)The potential of using hair cortisol to measure chronic stress in occupational 81. healthcare; a scoping review. J Occup Health. 2021 Jan;63(1):e12189. doi: 10.1002/1348- 82. 9585.12189. PMID: 33426766; PMCID: PMC7797775.
36. Sporkert F and Pragst F (2000).Use of headspace solid phase micro extraction (HS-SPME) in hair analysis for organic compounds. Forensci.INt107(1-3)129-148.
37. Stafford L. Lightman, Crispin C. Wiles, Helen C. Atkinson, David E. Henley, Georgina M.
38. Russell, Jack A. Leendertz, Mervyn A. McKenna, Francesca Spiga, Susan A. Wood, Becky L.
39. Conway-Campbell (2008). The significance of glucocorticoid pulsatility European Journal of Pharmacology, Volume 583, Issues 2–3, Pages 255-262, ISSN 0014-2999,
40. Tejashwini Huchannevar, Sunil Nayak, Shrath H P, Rajesh Shenov (2025).An overview of DHEs levels in chronic stress and BMI-A prospective study. Journal of Allied Health Sciences. Volume 5, Issue 1 https://doi.org/10.26463/rjahs.5_1_4.
41. Wei Zhang, Andrea Hetzel, Bijal Shah, Derek Atchley, Shannon Blume(2014).Greater 93. Physiological and Behavioral Effects of Interrupted Stress Pattern Compared to Daily Restraint Stress in Rats. Plos One 9(7):e102247.
42. Wosu C.A, Valdimarsdottar Unnur, Alexandra E Shields, Willams David , Michelle Awillams 96. (2014). Correlates of cortisol in Human Hair: Implications for epidemiology studies on Health 97. effects of chronic stress. Annals of Epidemiology 5;23(12):797–811.e2. doi: 10.1016/j.annepidem.2013.09.006.
43. Yousufzai, M.I.u.A., Harmatz, E.S., Shah, M(2018). Ghrelin is a persistent biomarker for chronic 100. stress exposure in adolescent rats and humans. Transl Psychiatry 8, 74 101. https://doi.org/10.1038/s41398-018-0135-5.

Hair Growth Pathway, Chronic Stress, Physical Stress, Chemical Stress, Stress Biomarkers.

1. Amina Inova ,Viktoria Birkenges, Daniel S Qiuntena, Monica Beg Ormered (2025).Cortisol and C- reactive protein (CRP) regulation in severe mental disorders. Psychrneuroendocrinology. Volume 172,107272 4. . [Google Scholar] [Crossref]

2. 5. Anna – karrin lennartsson, Elin Anvidson, Mats –Borjesson , Inigibing-Jonsdotin (2022). DHEA-S 6. production capacity in relation to perceived prolonged stress The International journal on the Biology of stress. Volume 25,Issue/Pages 105-112. [Google Scholar] [Crossref]

3. Arielo Niraulo, Kristno G withcher, John F, Sheridon, Jonathan P.Godboat (2019).Interleukin-6 9. Induced by social stress promotes a unique transcriptional signature in the monocytes that 10. facilitates anxiety biological psychiatry volume 85,Issue 8.Page 679-689. [Google Scholar] [Crossref]

4. Cay M, Ucar C, Senol D, Cevirgen F, Ozbag D, Altay Z, Yildiz S.(2018) Effect of increase in cortisol level due to stress in healthy young individuals on dynamic and static balance scores. [Google Scholar] [Crossref]

5. North Clin Istanb. 2018 May 29;5(4):295-301. doi: 10.14744/nci.2017.42103. PMID: 30859159; PMCID: PMC6371989. [Google Scholar] [Crossref]

6. Das, A.(2019). Chronic Ongoing stressors and c-reactive protein: A within – Person Study. [Google Scholar] [Crossref]

7. Journal of Aging and Health. Volume 32. Issue 7-8. [Google Scholar] [Crossref]

8. Bobko, N.A. (2007)Effect of stress on the cardiovascular system activity in operators of 18. predominantly mental work at different times of the day and the working week. Hum Physiol 33, 302–308. https://doi.org/10.1134/S0362119707030073. [Google Scholar] [Crossref]

9. Bouillon-Minois JB, Trousselard M, Thivel D, Gordon BA, Schmidt J, Moustafa F, Oris C, Dutheil F. Ghrelin as a Biomarker of Stress: A Systematic Review and Meta-Analysis. (2021). [Google Scholar] [Crossref]

10. Nutrients. Feb 27;13(3):784. doi: 10.3390/nu13030784. PMID: 33673594; PMCID: PMC7997253. [Google Scholar] [Crossref]

11. Christine Fahrngruber. Velasquez, Kalina Duszka and Jurgen Konig(2021).The impact of chronic stress and Eating concern on Acylased Ghrelin following Acute Psychological stress in Healthy Men.Stresses1(1),16-29. [Google Scholar] [Crossref]

12. Eleanor Mclaughan, Julian Barth H (2017). An analysis of the relationship between serum cortisol and serum sodium in routine clinical patients.Practical Laboratory Medicine. [Google Scholar] [Crossref]

13. Eva MJ peters,Yvonne Muller,Wenke Snaga,Herbert Fliege,Anett Reishaver,Thomas – Schmidt Rose(2017).Hair and stress: A pilot study of hair and cytokine balance alteration in healthy young women under major exam stress. Plos One.10.1371/Journal pone.0175904 [Google Scholar] [Crossref]

14. Golbidi S, Frisbee JC, Laher I(2015).Chronic stress impacts the cardiovascular system: animal models and clinical outcomes. Am J Physiol Heart Circ Physiology .308:1476-1498. [Google Scholar] [Crossref]

15. Gong S, Miao YL, Jiao GZ, Sun MJ, Li H, Lin J, Luo MJ, Tan JH. (2015).Dynamics and correlation 33. of serum cortisol and corticosterone under different physiological or stressful conditions in mice. PLoS One. 2015 Feb 20;10(2):e0117503. doi: 10.1371/journal.pone.0117503. PMID: 25699675; PMCID: PMC4336318. [Google Scholar] [Crossref]

16. Hwee-Soo Jeong (2018). The status of hair minerals in chronic fatigue and depressed women. [Google Scholar] [Crossref]

17. Asian journal of pharmaceutical and clinical research. Vol 11, Issue 11,485-488 [Google Scholar] [Crossref]

18. Jitendia R Dave, Sally M. Anderson, George A Saviolaks, Edward H.Mougey(2000).Chronic sustained stress increases levels of anterior pituitary prolactin mRNA, Pharmacol Biochem Behaviour 67(3):423-31. [Google Scholar] [Crossref]

19. Kudiela BM, Broderick JE, Kirschbaum C (2003). Compliance with saliva sampling protocols: electronic monitoring reveals invalid cortisol daytime profiles in noncompliant subjects. Psychomatic medicine 65(2):313-9.PMID:12652000. [Google Scholar] [Crossref]

20. Lauren A Stone, Elia S Harmatz, Ki A Grosens(2020)Ghrelin as a stress hormone; Implications for psychiatric illness. Biological Psychiatry .Volume 88, Issue 7,Page 531-540. [Google Scholar] [Crossref]

21. Lightman SL, Wiles CC, Henley DE, Wood SA, Wood SA (2008). The significance of glucocorticoid pulsatility. Eur J Pharmacol; 583:255-262. [Google Scholar] [Crossref]

22. Margheritta sisto and francesc Grans (2024). Immunology of stress: A review Article. J clin Med;13(21):6394. [Google Scholar] [Crossref]

23. Mahmut Cay, Cihat Ucar, Deniz Senol, Furkan Cevigran (2018). Effects of increase in cortisol 51. level due to stress in healthy young individuals on dynamic and static balance scores.Northern clinics of Istanbul May 29;5(4):295-301. [Google Scholar] [Crossref]

24. Melissa Dong, Matthew T. McGoldrick, Heather Seid, Laura P. Cohen, Ariana LaRocca, Patrick Pham, S. Justin Thomas, Joseph E. Schwartz, Daichi Shimbo(2022). [Google Scholar] [Crossref]

25. The stress, salexcretion, and nighttime blood pressure (SABRE) study: Rationale and study design, American Heart 56. Journal Plus: Cardiology Research and Practice, Volume 13,100099, ISSN 2666- 6022,https://doi.org/10.1016/j.ahjo.2022.100099. [Google Scholar] [Crossref]

26. Nabill Ibrahim, Esam Eweis, Hossam El-Beltagi, Yasmin Abel-Mobby(2012).Effect of lead 59. acetate toxicity on experimental male albino rat. Asian Pacific Journal of tropical Biomedicine ; 2(1):41- 46. [Google Scholar] [Crossref]

27. Parasuraman S,Ravendran R,Kesavan R(2010)Blood Sample collection in small laboratory animals.J harmacol,87-93. [Google Scholar] [Crossref]

28. Piruzian, L.A., Protasova, O.V., Maksimova, I.A. (2014).Calcium and phosphorus contents of human body during 105-day isolation. Hum Physiol 40, 822–826. https://doi.org/10.1134/S0362119714070226. [Google Scholar] [Crossref]

29. Qing H, Desrouleux R, Israni Winger K, Mineur YS, Fogelman N, Zhang C, Radheed S, Palm NW ,Sinta R(2020).Origin and function of stress – Induced IL-6immune models cell.cell.com P1660 Pubmed 372387, volume 182,Issue 6 [Google Scholar] [Crossref]

30. Ren J, Guo J, Zhu S, Wang Q, Gao R, Zhao C, Feng C, Qin C, He Z, Qin C, Wang Z, Zang L.(2021)The Role of Potassium Channels in Chronic Stress-Induced Brain Injury. Biol Pharm Bull. 2021 Feb 1;44(2):169-180. doi: 10.1248/bpb.b20-00504. Epub 2020 Nov 25. PMID: 33239494 [Google Scholar] [Crossref]

31. Samara Levine, Ozgul Muneyeriei (2018). Stress Induced hyperprolactinemia: pathophysiology and clinical Approach. Obstet Gynecol Int.Doi:10.1155/2018/9253083 [Google Scholar] [Crossref]

32. Sanjeevs s. walvekar, Jeevan G. Ambekah, Basavaraj B(2015).Study on serum cortisol and 74. perceived stress scale in the police constables. Journal of clinical and Diagnostic Research Vol.9(2):BCID – BC14. [Google Scholar] [Crossref]

33. Satoh E, Tada Y, Matsuhisa F.(2011) Chronic stress enhances calcium mobilization and glutamate exocytosis in cerebrocortical synaptosomes from mice. Neurol Res. 2011 Nov;33(9):899-907. doi: 10.1179/1743132811Y.0000000033. PMID: 22080989. [Google Scholar] [Crossref]

34. Schaafsma FG, Hulsegge G, de Jong MA, Overvliet J, van Rossum EFC, Nieuwenhuijsen K. [Google Scholar] [Crossref]

35. (2021)The potential of using hair cortisol to measure chronic stress in occupational 81. healthcare; a scoping review. J Occup Health. 2021 Jan;63(1):e12189. doi: 10.1002/1348- 82. 9585.12189. PMID: 33426766; PMCID: PMC7797775. [Google Scholar] [Crossref]

36. Sporkert F and Pragst F (2000).Use of headspace solid phase micro extraction (HS-SPME) in hair analysis for organic compounds. Forensci.INt107(1-3)129-148. [Google Scholar] [Crossref]

37. Stafford L. Lightman, Crispin C. Wiles, Helen C. Atkinson, David E. Henley, Georgina M. [Google Scholar] [Crossref]

38. Russell, Jack A. Leendertz, Mervyn A. McKenna, Francesca Spiga, Susan A. Wood, Becky L. [Google Scholar] [Crossref]

39. Conway-Campbell (2008). The significance of glucocorticoid pulsatility European Journal of Pharmacology, Volume 583, Issues 2–3, Pages 255-262, ISSN 0014-2999, [Google Scholar] [Crossref]

40. Tejashwini Huchannevar, Sunil Nayak, Shrath H P, Rajesh Shenov (2025).An overview of DHEs levels in chronic stress and BMI-A prospective study. Journal of Allied Health Sciences. Volume 5, Issue 1 https://doi.org/10.26463/rjahs.5_1_4. [Google Scholar] [Crossref]

41. Wei Zhang, Andrea Hetzel, Bijal Shah, Derek Atchley, Shannon Blume(2014).Greater 93. Physiological and Behavioral Effects of Interrupted Stress Pattern Compared to Daily Restraint Stress in Rats. Plos One 9(7):e102247. [Google Scholar] [Crossref]

42. Wosu C.A, Valdimarsdottar Unnur, Alexandra E Shields, Willams David , Michelle Awillams 96. (2014). Correlates of cortisol in Human Hair: Implications for epidemiology studies on Health 97. effects of chronic stress. Annals of Epidemiology 5;23(12):797–811.e2. doi: 10.1016/j.annepidem.2013.09.006. [Google Scholar] [Crossref]

43. Yousufzai, M.I.u.A., Harmatz, E.S., Shah, M(2018). Ghrelin is a persistent biomarker for chronic 100. stress exposure in adolescent rats and humans. Transl Psychiatry 8, 74 101. https://doi.org/10.1038/s41398-018-0135-5. [Google Scholar] [Crossref]