Aqueous and Methanolic Extract of Ocimum Gratissimum (Linn.) Leaf Reversibly Normalizes the Antioxidant Activities of Rats with Gentamicin-Induced Liver Injury
- September 30, 2019
- Posted by: RSIS
- Categories: IJRSI, Medical Sciences
International Journal of Research and Scientific Innovation (IJRSI) | Volume VI, Issue IX, September 2019 | ISSN 2321–2705
Oluwadare J. Ogundipe1, Risquat O. Olaleye 1, Christian E. Imafidon1, Olaoluwa S. Olukiran1, Omolola F. Akinpelu2, Adedapo J. Sanusi3, A. Oyerinde4, Oyelade R. Oluwakemi5
1Department of Physiological Sciences, Faculty of Basic Medical Sciences, Obafemi Awolowo University, Ile – Ife, Osun State, Nigeria
2Department of Anatomy, Faculty of Basic Medical Sciences, Olabisi Onabanjo University, Ago – Iwoye, Ogun State, Nigeria
3Department of Biochemistry, Faculty of Sciences, Obafemi Awolowo University, Ile – Ife, Osun State, Nigeria
4Department of Physiology, Faculty of Basic Medical Sciences, Lead city University, Ibadan, Oyo State, Nigeria
5Department of Physiology, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Oyo State, Nigeria
Abstract:-
Objective: the objective of the work is to investigate the ameliorative effect of aqueous and methanolic extract of ocimum gratissimum (linn) on gentamicin-induced liver injury. Forty five adult male Wistar rats were used for this study. They were divided into 5 groups as follows: Group 1 (the control) (n = 5) received distilled water daily by oral route for the whole period of the study. Group 2 (the toxic control) (n = 10) received 100 mg/kg/day of gentamicin i.p. for a week. Groups 3, 4, and 5 (n = 5) were pre-treated with gentamicin as the Group 2 rats, after which they received 100, 200 and 400 mg/kg/day each of AOGL p.o., Groups 6, 7, and 8 (n = 5) were pre-treated with gentamicin as the Group 2 rats, after which they received 100, 200 and 400 mg/kg/day each of MOGL p.o., respectively, for 14 days. Markers of liver function such as ALT, AST, ALP, Total bilirubin, conjugated bilirubin and total protein were determined in the plasma. Oxidative stress markers such as TBARS and GSH were assayed in the tissue homogenate. Gentamicin treatment induced significant (p < 0.05) increases in ALT, AST, ALP, Total bilirubin and total protein and TBARS in the toxic control when compared to the control group. Significant decreases (p < 0.05) in GSH was also associated with gentamicin administration. Post-treatment with AOGL caused significant increases in and GSH, and significant (p < 0.05) decreases in ALT, AST, ALP, Total bilirubin and total protein in the treated groups when compared with the toxic control group. The results of this study indicated that AOGL ameliorated the liver injury caused by gentamicin in rats. Hence, the extracts have the potential of being used for the management of gentamicin-induced liver injury.
Keywords: Ocimum gratissimum leaves extract; Gentamicin; ALT; AST; ALP; Total protein; TBARS; GSH; Liver; rats.
