Bioreporters as Novel Analytical Sensing Tools
- September 1, 2020
- Posted by: RSIS Team
- Categories: Applied Science, IJRIAS
International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume V, Issue VI, June 2020 | ISSN 2454-6194
Bioreporters as Novel Analytical Sensing Tools
Tony Oyigbeche1, Olukayode Olugbenga Orole1, Victor Stephen Fadayomi2
1Department of Microbiology, Federal University of Lafia, Nigeria
2Department of Zoology, Federal University of Lafia, Nigeria
Abstract- A bioreporter is a living microorganism containing a sensor molecule that upon binding of a small molecule of interest switches on a reporter, resulting in a measured cellular signal outputs which can be a colorimetric, bioluminescent, or fluorescent emission. They are very specific for the target chemical molecule. The use of bioreporters in detecting target molecules lies in altering the transcriptional regulator so as to change the specificity. Bioreporters are applied in water quality control and assessment, identification of pathogenic organisms of human health concern, to establish toxicity profiles in environmental samples, specific detection of pollutant and heavy metals, determine bioremediation rates, search for novel biocatalysts, and to improve strains for industrial production of small molecules. They are easy to use, rapid, adaptive, and robust tool for chemical analysis. The review highlights the type of bioreporters currently in use, mechanism of switch on and off, and their applications.
Keywords: Analysis, Bioreporters, chemical, Sensors, emission
