Cytotoxic Pyrrolidinone from Leaves of Jatropha tanjorensis

International Journal of Research and Scientific Innovation (IJRSI) | Volume V, Issue IX, September 2018 | ISSN 2321–2705

Cytotoxic Pyrrolidinone from Leaves of Jatropha tanjorensis

Madepalli Byrappa Gowdu Viswanathan¹, Jesu Doss Jeya Ananthi², Nata Rajan Livingston Raja², and Narayanan Venkateshan²

¹ Department of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India
² Departments of Pharmaceutics, Arulmigu Kalasalingam College of Pharmacy, Krishnankoil – 626126, Tamil Nadu, India

Abstract-Cancer metastasis, which involves multiple processes and various cytophysiological changes, is a primary cause of cancer death. Currently available therapeutic drugs have limited effects on metastasis tumors. Therefore, there is an urgent need for novel therapeutic approaches to treat tumor metastasis. Search for effective agent from plant resources, such as flavonoids or alkaloids for the treatment of cancer metastasis has become one of the top priorities in cancer research. The aim of the present study is to study the cytotoxic effects of leaves on Jatropha tanjorensis by in vitro and in vivo methods. In vitro cytotoxic properties of methanol extract and R(+) 4-hydroxy-2-pyrrolidinone of J. tanjorensis were taken up against 4 cancerous cell lines, viz., HEP-2, B16F10, A549, and NRK 49F using standard procedure.  According to Hartwell latex sap and leaf of species of Jatropha are used in warts and tumors.  This plant has not been studied for their cytotoxic effect. The methanol fraction of J. tanjorensis was studied for in vivo cytotoxic activity using B₁₆F₁₀ melanoma cells in mice.  Simultaneous administration of methanol fraction at the doses of 100 and 200 mg/kg and R(+) 4-hydroxy-2-pyrrolidinone per oral. The methanol extract and R (+) 4-hydroxy-2-pyrrolidinone exhibited more cytotoxic activity against all the 4 cell lines by in vitro study.  The Caucasian male larynx epithelium cornicing Hep-2 was found to be more susceptible with an IC₅₀ value of 43.24μg/ml.  The other cell line showed less activity as indicated by the relatively high IC₅₀ value. The results in vivo study revealed that R (+) 4-hydroxy-2-pyrrolidinone exhibited relatively high increase in life span than methanol fraction

Key words: Jatroph tanjorensis, R (+) 4-hydroxy-2-pyrrolidinone in- vitro & in- vivo cytotoxic activity

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