Effects of Hydromethanolic Extract of Cnidoscolus Aconitifolius (Buphorbiacaea) on Body Weight, Some Liver Enzymes and Histology in Diabetic Wistar Rats

International Journal of Research and Scientific Innovation (IJRSI) | Volume VI, Issue IX, September 2019 | ISSN 2321–2705

Effects of Hydromethanolic Extract of Cnidoscolus Aconitifolius (Buphorbiacaea) on Body Weight, Some Liver Enzymes and Histology in Diabetic Wistar Rats

Ikechukwu I. Weleh¹ and Friday Saronee^1*

 ¹Department of Human Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Port Harcourt, Choba, Port Harcourt, Nigeria
*Corresponding Author

Abstract-This study evaluated the effect of hydromethanolic leaf extract of Cnidoscolus Aconitifolius (C.A) (euphorbiaceae) on body weight, some liver enzymes and histology in Streptozotocin (STZ) induced diabetic Wistar rats. Thirty-six wistar rats of both sexes with an average weight of 230g were divided into six groups of 6 rats each. The rats were fasted for 12 hours and diabetes induced intraperitoneally using 60mg/kg-l b.w of STZ. Group l: Untreated non-diabetic, Group 2 received 10mg of glibenclamide, Group 3: Untreated diabetic, served as the positive control. Groups 4, 5 and 6 were treated with 100, 150 and 200 mg/kg b.w of the extract respectively. On day 29, the animals were anesthetized using chloroform and sacrificed. The body weight of animals was determined using an electric weighting balance and blood samples collected by cardiac puncture for the determination of some liver enzymes, while the liver were collected and fixed in 10% formalin for histological studies. Results show that the extract significantly increase the body weight of experimental animals in a dose dependent manner in the test groups (p<0.05). There was also a significant increase in liver enzymes in groups 4, 5 and 6 compared to Groups 2 and 3 (p<0.05). Histological studies show some levels of degenerations in the tissues of the liver. Result suggest that C.A could encourage weight gain but have adverse effect on the liver as seen in the elevated serum liver enzymes supported by the histological studies.

Key words: Cnidoscolus Aconitifolius, Streptozotocin, diabetes, hydromethanolic, chloroform.

I. INTRODUCTION

Weight gain is an increase in body weight: this can involve an increase in muscle mass, fat deposits, excess fluids such as water or other factors [1]. Maintaining a healthy weight is important for health. In addition to lowering the risk of heart disease, stroke, diabetes, and high blood pressure, it can also lower the risk of many different cancers [2].

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