Ex vivo Determination of Prostate Specific Antigen (PSA) Using Green Synthesized Magnetic Iron-Oxide Nanoparticles (IONPS)

International Journal of Research and Scientific Innovation (IJRSI) | Volume VII, Issue IV, April 2020 | ISSN 2321–2705

Ex vivo Determination of Prostate Specific Antigen (PSA) Using Green Synthesized Magnetic Iron-Oxide Nanoparticles (IONPS)

Uchenna, Muhd BK^1*, Umar AA², Muhammad Y.³, Kani YA.⁴, Iliya S.⁵, Wali U.⁶, Tahiru A.⁷, Abubakar UF⁸, Shehu Z.⁹, Ahmed AY.¹⁰, Zainab I^11
1,2Department of Biotechnology Federal University Dutse PMB 7156, Nigeria
^3,7Department of Chemical pathology, Rasheed Shekoni Specialist Hospital Dutse, Nigeria
⁴College of Medicine and Health Sciences, Federal University Dutse, Nigeria
⁵Department of Biological Sciences, School of Pure and Applied Sciences, Mount Kenya University Thika, Kenya
^6,9Department of Chemical Pathology Faculty of Medical Laboratory Science, Usmanu Danfodiyo University Sokoto, Nigeria
⁸Public Health and Diagnostic Institute, Yusuf Maitama Sule University Kano, Nigeria
¹⁰Department of Science Laboratory Technology, Jigawa State Polytechnic, Nigeria
¹¹Department of Hematology and Blood Group Serology, Rasheed Shekoni Specialist Hospital Dutse, Nigeria
*Corresponding author

Abstract: -The discovery and introduction of prostate-specific antigen (PSA) testing into clinical medicine enable the diagnosis, treatment and monitoring of prostatic carcinoma, and further revolutionized the management of patients with prostate cancer. Among the current developments in molecular detection of PSA is ELISA. The current research aimed at synthesizing the magnetic Iron-oxide nanoparticles for detection (assay) of prostate specific antigen and comparing the results obtained with that of the ELISA technique. It provides new advancements in molecular detections of PSA using magnetic iron-oxide nanoparticles. The magnetites were green synthesized using blackberry leaves extract, FeCl₂.4H₂O and FeCl₃.6H₂O. Serum samples (n=30) were treated and subjected to both ELISA and magnetic iron-oxide nanoparticles immunoassay techniques. Descriptive statistical analysis (α=.05) of T-test, chi-square and ANOVA using SPSS software package, reveals (p<0.005) no significance difference between values generated from ELISA and magnetic iron-oxide nanoparticles techniques. Thus, the null hypothesis which asserts that, there is no significant difference between ELISA and magnetic iron-oxide nanoparticles in PSA assay fails to be rejected. It is therefore recommended that magnetic iron-oxide nanoparticles be further exploited with regards to ex vivo determination of PSA and other tumor markers.

Key words: Magnetic Iron-Oxide Nanoparticles (IONPS), Prostate specific antigen (PSA), Assay, ELISA.

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