GC-MS Analysis of Some Phytochemical Constituents in Stem Bark of Khaya Senegalensis

International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume V, Issue IV, July 2020 | ISSN 2454-6194

GC-MS Analysis of Some Phytochemical Constituents in Stem Bark of Khaya Senegalensis

(Desr.) A. Juss Mohammed Idris1 , Adamu Ibrahim Inuwa1 , Aida Mohammed Kajiama2 , Shehu Ibrahim Baburo3
1Department of Chemistry, Faculty of Science, Federal University Gashua, Nigeria
2 Shehu Sule School of Nursing and Midwifery, Damaturu, Nigeria
3Department of Basic Science, Yobe State College of Agriculture, Gujba, Nigeria
*Corresponding Author

Abstract: The phytochemical constituents of crude ethanolic stem bark extracts of khaya senegalensis were isolated and analyzed using gas chromatography-mass spectrometry (GC-MS). A shade dried stem bark of khaya senegalensis was extracted using ethanol solvent. The crude extract was subjected to preliminary phytochemical analysis in order to confirm the various classes of secondary metabolites present using standard procedure, and this indicated the presence of alkanoid, flavonoids, tannins, terpenoids, steroids, saponins, and carbohydrate. The GC-MS analysis of crude ethanolic extract of the sample using coupled Agilent 7890B gas chromatography and Agilent 5977A mass spectrometer confirmed the presence of: Methylamine, N, N-dimethyl-; N, NDimethylotylamine; 1-Hexene, 3,4- dimethyl; 1-nonanol; Benzaldehyde, 3-benzyloxy-2-fluoro-4-methoxy; 7- Tetradecene; Bicyclo [2.1.1] hexane-2-ol, 2-ethenyl; 4- chloro-3-n-hexyltetrahydropyran; 5-ethyl-5-methyl-2- phenyl-2-oxazoline; Cis 5, 8, 11, 14, 17-Eicosapentaenoic acid; 𝟐, 𝟒′-Dihydroxydiphenylsulphone; Phenylphosphonous acid; 3-Hydroxy-2,5,5,8atetramethyl-3,4,4a,5,6,7,8,8a-octahdronaphthalene-1- carboxylic acid, methyl ester; Extran-3-one,17-hydroxy- (5α,17β); i-propyl 9-hexadecanoate; Octahydrobenzo[b]furan, 2-cyclohexylimino 7a-methyl; Germacrene A, 9-(methylthio)-; 3-hydroxy-2,5,5,8a-tetrametyl3,4,4a,5,6,8,8a-octahydronaphthalene-1-carboxylic acid, methyl ester; 5-[(2,4-Dinitrophenyl)-hydrazonomethyl]- dihydrofuran-2-one. for minor peaks. The molecular weight of these compounds ranged from low to high with carbon skeleton of between C3 and C20 that are both aromatic and aliphatic.

Keywords: khaya-senegalensis, GC-MS, estradiol, stembark, chromatogram.

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