International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume V, Issue III, March 2020 | ISSN 2454–6186

Public Health Implications of Locally Femented Milk (Nono) and Antibiotic Susceptibility Testing of Pseudomonas Aeruginosa Isolated From The Product in Borokiri , Rivers State Nigeria

 Wiri, Thankgod Bariyaa; Osumenya, Inimeya Thompson; Gbode, Yenor Lekia
Department of Environmental Health Technology, Rivers State college of Health Science and Management Technology Port Harcourt, Nigeria

Abstract:- The study is to determine the PH and moisture content of Nono sold in Port Harcourt , the prevalence of Pseudomonas aeruginosa in Fura da nono and finally the antibiotic resistance pattern of Pseudomonas aeruginosa isolated from the fermented products. nono samples were purchased from Borikiri in portharcourt township. A total of 20 samples were assessed to determine their microbiological quality and to conduct antibiotic susceptibility test. Moisture content and pH of the samples were also assessed. Enumeration of the total viable bacterial count (TVBC), Total coliform count (TCC) and Total Pseudomonal count (TPC) were also assessed to determine the sanitary quality of the product. The PH ranges between 2.99 to 3.89 while the moisture content ranges between 80% to 88%. The result obtained from the microbial culture indicated that a wide array of microorganism were present in Fura da nono including species of Bacilu, klebsiella, Pseudomonas Staphylococcus aureus, Streptococcus, Lactobacillus and Escherichia coli.. The highest TVBC, TCC and TPC were 9.8×103 cfu/ml, 10×103 cfu/ml and 9.7x103cfu/ml respectively. Antibiotic susceptibility was conducted using 12 broad spectrum antibiotics and compared against a standard provided by the Clinical laboratory standard institute (CLSI). Gentamycin, Ofloxacin and Levofloxacin recorded 100% resistance , while Cotrimoxazole, Ciprofloxacin, Vancomycin, Nitrofurantoin, Norfloxacin and Azithromycin recorded 100% susceptibility as indicated by the complete clear zone of inhibition.It was discovered that the absence of regulatory agencies like National Agency for Food Drug Administration and Control (NAFDAC) in the regulation of the quality of the product was the cause of the high contamination, since there were no quality control measures in its production line .It was recommended that NAFDAC should provide a standard operating procedure for local food producers and should include them in their scope for regulation.

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