Serum Biochemistry Profiles, Haematological Indices and Body Weight Gains of Albino Rats Fed Makurdi Dakuwa Meal (MDM)
- May 11, 2019
- Posted by: RSIS
- Category: Food Science and Technology
International Journal of Research and Scientific Innovation (IJRSI) | Volume VI, Issue IV, April 2019 | ISSN 2321–2705
Ikya, J.K.1*, Mzahan, E. H.1, Shalem, S.1
1Department of Food Science and Technology, Federal University of Agriculture, Makurdi, Nigeria
*Corresponding Author
Abstract:-Three samples of Makurdi Dakuwa Meals (MDM) purchased from Wurukum, Wadata and North Bank in Makurdi metropolis were subjected to proximate composition and microbiological analyses. The samples were also fed albino rats for twenty-eight (28) days in a feeding trial experiment and their blood samples were randomly collected and subjected to Serum Biochemistry Profiles and Haematological Indices analyses. Each of the experimental rats’ group were daily fed with weighted thirty (30)g of Makurdi Dakuwa Meal (MDM) and their weight gain recorded and compared with their initial weight to determine body weight gain in a statistical completely randomized design (CRD). Each of the three groups was further subdivided into three replicates of three albino rats each. The results of the proximate composition of the Dakuwa Meals (MDM) samples from A (Wurukum), B (Wadata) and C (North Bank) showed that ash ranged from 2.4% – 3.2%, crude fibre 1.4% – 1.45% and carbohydrate by difference was 47.95% – 52.79% not significantly different (p > 0.05) from one another. The moisture contents of the samples were between 10.5% – 11.5% with the samples from Wadata having significantly (p<0.5) higher moisture than those from Wurkum and North Bank. The protein content of the samples varied significantly (p < 0.05) and was highest (19.69%) in the North bank sample and lowest (11.16%) in the Wadata sample. The fat content of the samples was also significantly different (p<0.05) with values ranging between 22% – 32%. The results of the microbiological qualities of the samples showed that the total bacterial counts of Dakuwa Meal (MDM) ranged from 2.0 x 104cfu/g – 2.1 x 104cfu/g. The total yeast and mould count of the samples were also significantly different (p < 0.05) and the values ranged from 2.0 x 102cfu/g – 6.5 x 102cfu/g respectively. Makurdi Dakuwa Meal (MDM) did not significantly affected (P > 0.05) the serum biochemistry profiles Wister albino rats fed three respective meals A, B and C were (cholesterol(mmol/L)1.76(A),1.74(B), 1.73(C), total protein(g/L) 10.70(A), 10.75 (B) and 10.73(C)Alanine amino Transferase-ALT (iu/L) A(1.51), B(1.53) and C(1.52) and Aspartate amino Transferase- AST(iu/L) A(2.72), B(2.73) and C(2.70) were not affected significantly (P > 0.05) by location of the Makurdi Dakuwa Meals (MDM). Haematological indices: Packed Cell Volume-PCV(%) A(39.67) B(39.65) and C (39.66),Red Blood Cell- RBC(1012/L) A (7.07), B(7.04)and (7.09) and White Blood Cell- WBC (109/L)A (2.56) B(2.58) and C(2.55) of Wister albino rats fed three respective meals A, B and C were not affected significantly (P > 0.05) by location of the Makurdi Dakuwa Meals (MDM). (P > 0.05). Body weight gains (g), 23.30, 23.36and 23.29 of the albino rats fedthree respectiveMakurdi Dakuwa Meals (MDM) from A (Wurukum), B (Wadata) and C (North Bank) were not significantly different (p > 0.05) from one another. Makurdi Dakuwa Meals (MDM) is healthy and recommended for human consumption.
Key words: Dakuwa Quality, Haematology, Serum Biochemistry, Albino Rats