- September 18, 2020
- Posted by: RSIS Team
- Categories: Animal Science, IJRIAS
International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume V, Issue III, January 2020 | ISSN 2454–6186
D. Kulawe1*, N. Ahidjo2 and J. S. Hena3
1Department of Biological Sciences, Gombe State University, Gombe, Nigeria
2Department of Biological Sciences, Gombe State University, Gombe, Nigeria
3Nigeria. Marine Environment Management Unit, Nigerian Maritime Administration and Safety Agency,Abuja Office, Nigeria
Abstract: – Combretum molle has been used by many traditional medicine practitioners in the treatment of microbial infections. This work was carried out with the aim of determining the acute toxicity and histology of the aqueous, n-butanol and the ethyl-acetate fractions of the root of Combretum molle to Wistar rats. Ethanol was used as solvent for extraction, after which differential fractionization was carried out using distilled water, ethyl acetate and n-butanol. The limittest at 5000 mg/kg of the Organization for Economic Cooperation and Development (OECD) guidelines were used for the study. In the acute toxicological investigation, there was no mortality in the experimental animals after orally administering the fractions of C. molle 5000 mg/kg indicating that the LD50 was above 5000 mg/kg. There was no histological alterations or changes at the extract dose of 5000 mg/kg body weight in the kidney organs of rats in the control group, but all the other organs from the fractions tested displayed certain observed alterations. Tubular vacuolation (TVN), Lymphocyte hyperplasia (LH), Glomerular necrosis (GN), Plaques formation (P), Tubular necrosis (TN) and Tubular distortions (TD) were observed in the kidneys. There was no histological alterations or changes at the extract dose of 5000 mg/kg body weight in the internal organs of rats in the control group and in the liver of group VI, but all the other organs from the fractions tested displayed certain observed alterations. Vascular congestion (VC), vascular congestion with slight necrosis (VCN) and hepatocellular necrosis (HN) were observed in the livers of all the groups administered the fractions.
Key words: Combretum molle, ethanol, acute toxicity, Wistar rats
