In vitro Regeneration protocol for Mulberry (Morus alba L.) through Tissue Culture Techniques

International Journal of Research and Innovation in Social Science (IJRISS) | Volume VI, Issue I, January 2022 | ISSN 2454–6186

In vitro Regeneration protocol for Mulberry (Morus alba L.) through Tissue Culture Techniques

Md. Sohel Rana^1*, Muhammad Nurul Amin² and Mustafa Abul Kalam Azad^2
1Regional Bamboo Research and Training Centre, Bangladesh Forest Research Institute, Domar, Nilphamari-5340, Bangladesh.
²Professor, Department of Botany, University of Rajshahi-6205, Bangladesh
*Corresponding author

Abstract: This research work was taken for enormous production of white mulberry (Morus alba L.) through micropropagation techniques. Surface sterilization of the explants collected from the field grown mature plants was important and it was done with 0.1% HgCl₂ (Mercuric chloride) solutionat different durations. Result showed that duration of the treatment was the best at 12 minutes for both shoot tips and nodal segments. Axillary shoot proliferation of nodal explants was better than that of (terminal) shoot tips. Data analysis showed that cytokinin BAP (6-Benzyl aminopurine) was more effective than Kn (Kinetin) for axillary shoot proliferation. The MS medium supplemented with 6.0 µM was the best to grow 100% shoot proliferation of the explants where the average length was 5.57cm ± 0.05. On the other hand, different concentration and type of auxin affected greatly to induce number and percentage of roots per shoot. In this case, half MS medium supplemented with 6.0 µM IBA (Indole-3-butyric acid) was showed the highest percentage (100%) of root regeneration where the average length was 4.21cm ± 0.07.
Key words: Axillary shoot, Sterilization, Micropropagation, Explants, Proliferation

I. INTRODUCTION

Mulberry are fast growing perennial plants which are small to medium sized shrubs. The highest tall of mulberry plant is about up to 24m (80ft) wide spreading and round-topped. The role of mulberry leaves as a protein source in food formulations is satisfactory and its neuroprotective functions are also effective against neurodegenerative disorders such as Alzheimer and Parkinsonism(Butt et al., 2008). A silkworm produces luxuriant silk thread to form cocoon from mulberry leaves during larval stage(Rahmathulla, 2012).The flowers are small size, greenish-yellow colour, 4 sepals, 4 stamens and pistils bearing two styles. An oval aggregate fruit turns into individual drupelets. The fruit containing numerous brown seeds is normally white, pink or purple-black in colour. Although, over 150 mulberry species is present in the world, botanists recognize only 10–16 species widely(Datwyler et al., 2004). Generally, all mulberries grow very fast for 40–50 years and it reach upto 60–80 cm in diameter at breast height (dbh), after which their growth rates are slow. The living period of mulberry tree is about 200–300 years(Lochynska et al., 2011).
Mulberry is generally distributed in tropical, subtropical, temperate and sub-arctic areas (Machii et al., 2002).China started mulberry cultivation and silk production about 3000

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