A Probe into the Academic Life Satisfaction of the School-Going Adolescents of Paschim Medinipur District
- Hirak Ranjan Bera
- Kajal Das
- Samirranjan Adhikari
- 667-675
- Sep 8, 2025
- Education
A Probe into the Academic Life Satisfaction of the School-Going Adolescents of Paschim Medinipur District
Hirak Ranjan Bera1, Kajal Das2, Samirranjan Adhikari3
1Research Scholar, Department of Education, Swami Vivekananda University, Barrackpore, West Bengal, India
2Assistance Professor, Swami Vivekananda University, Barrackpore, West Bengal, India
3Professor, Department of Education, Sidho-Kanho-Birsha University, Purulia, West Bengal, India
DOI: https://doi.org/10.51584/IJRIAS.2025.100800058
Received: 25 August 2025; Accepted: 30 August 2025; Published: 08 September 2025
ABSTRACT
To assess the academic life satisfaction of school-going adolescents, this study employed a descriptive survey method. The Multidimensional Students’ Life Satisfaction Scale (Huebner et al., 1998) was administered to a randomly selected sample of 500 adolescents from schools in the Paschim Medinipur district. The findings revealed that life satisfaction derived from family was exceptionally high, while satisfaction related to school, friendships, and self-perception was also high. Overall, the total life satisfaction of the adolescents was found to be high. However, satisfaction with the living environment was notably low. Female school-going adolescents reported higher academic life satisfaction compared to their male counterparts.
Keywords: Academic Life Satisfaction, Life Satisfaction, Positive Psychology, Quality of Life, Subjective Well-Being
INTRODUCTION
Adolescence is a crucial developmental stage characterised by cognitive, emotional, and social changes that significantly influence academic motivation and overall well-being (Steinberg, 2014). Academic life satisfaction, a key aspect of adolescent well-being, is a multidimensional construct encompassing satisfaction with family, friendships, school environment, self-perception, and living conditions (Huebner et al., 2005). Positive academic experiences contribute to students’ motivation, self-concept, and psychological well-being, which in turn impact their future aspirations and success (Diener, 2000).The Paschim Medinipur District, with its diverse socio-cultural and educational landscape, provides an important context to examine the academic life satisfaction of school-going adolescents. Research suggests that family support, peer relationships, and school engagement play a critical role in shaping students’ academic experiences (Eccles & Roeser, 2011). A supportive school environment with strong teacher-student interactions fosters motivation and learning, while a positive home atmosphere enhances emotional stability and academic confidence (Ryan & Deci, 2000). However, external factors such as the quality of the living environment may also influence adolescents’ academic satisfaction, potentially affecting their engagement and well-being (Bronfenbrenner, 1979).This study aimed to explore the various dimensions of academic life satisfaction among adolescents in Paschim Medinipur, identifying key strengths and areas of concern.
Objective of the Study
The broad objectives of this study were –
- To observe the present state of affairs of the Academic Life Satisfaction of school-going adolescentsof Paschim Medinipur District;
- To compare the Academic Life Satisfaction of the male and female school-going adolescents.
The Hypotheses were –
H1: The school-going adolescents, considering both male and female as a whole, have high Academic Life Satisfaction.
H2: The male and female school-going adolescents do not differ with respect to their Academic Life Satisfaction.
CONSTRUCT OF THE STUDY: ACADEMIC LIFE SATISFACTION
Academic life satisfaction refers to a student’s overall contentment and fulfilment with their educational experiences, including their academic performance, learning environment, and relationships with teachers and peers. The Multidimensional Students’ Life Satisfaction Scale assesses students’ satisfaction across various life domains, including family, friends, school, living environment, and self (Huebner, 1994; Huebner et al., 1998). Family life satisfaction encompasses a student’s contentment with their family relationships and dynamics. In contrast, friends’ life satisfaction pertains to a school-going adolescent’s satisfaction with their peer relationships. School life satisfaction refers to a student’s enjoyment and fulfilment with educational activities, learning, and overall school experience. Furthermore, self-satisfaction involves a student’s self-perceived worth and satisfaction with others’ opinions about them, reflecting the concept of the “looking-glass self”. Lastly, living environment life satisfaction concerns a student’s satisfaction with their immediate community, neighbourhood, and local environment.
A BRIEF REVIEW OF ACADEMIC LIFE SATISFACTION
The studies mentioned provide a comprehensive overview of various factors influencing life satisfaction and self-esteem among adolescents, highlighting the critical roles of gender, parental support, peer communication, and extracurricular activities. Mistry et al. (2024) found that female adolescents reported significantly higher life satisfaction in areas such as family, friends, school, and self-compared to their male counterparts, although satisfaction with living conditions showed no gender differences. Radhika (2024) explored the relationship between life satisfaction and self-esteem, revealing that adolescents with high life satisfaction also exhibit higher self-efficacy in academic, social, and emotional domains. Gender influences both life satisfaction and self-esteem, with academic success and self-esteem acting as predictors of life happiness for both genders. Char et al. (2023) and Manna (2023) further illustrate how supportive family environments in the Purulia District and varying family structures influence adolescents’ life satisfaction and self-esteem. Zuo et al. (2022) emphasised the mediating role of physical activity in enhancing life satisfaction through increased parental support. The findings of Saad et al. (2020) showed strong links between life satisfaction and self-esteem among Indian and Afghan students, with gender differences influencing these outcomes.
Additional studies, such as those by Rath and Patra (2018), Clarke et al. (2023), and Supervía et al. (2023), underline the importance of parental attachment, eudaimonia, and self-efficacy in shaping adolescents’ life satisfaction. The role of self-esteem as a mediator between life satisfaction and other psychosocial factors is particularly noteworthy. Research by Lábiscsák-Erdélyi et al. (2022) and Šimunović and Olčar (2022) highlights how school-related factors and participation in extracurricular activities like sports and music programs contribute to life satisfaction, with gender playing a significant role in these dynamics. Lastly, Szcze´sniak et al. (2022) and Gallego et al. (2021) demonstrate how peer communication and emotional intelligence are crucial for understanding the complex relationships between life satisfaction, self-esteem, and overall adolescent well-being.
METHODS
The present study was carried out through the Descriptive Survey Method.
Variables
Academic Life Satisfaction was the only variable of the present study.
Sample
To make the sample representative multiphasic stratified random sampling technique was adopted. In the present study, 500 school-going adolescents aged 14-16 years were studied. Studying in the 9th and 10thstandards were randomly selected from 11 Government Sponsored Secondary and Higher Secondary Schools (Bengali medium) of Paschim Medinipur District, West Bengal.
The Tool of Research
Multidimensional Students Life Satisfaction Scale (MSLSS), (Huebner, Laughlin, Ash & Gilman, 1998)
The Multidimensional Students’ Life Satisfaction Scale (MSLSS) is a widely used research instrument developed by Huebner, Laughlin, Ash, and Gilman in 1998. It assesses students’ satisfaction with various aspects of their lives, typically across five domains. The MSLSS consists of 40 items, with respondents rating their satisfaction on a 5-point scale. This scale provides a comprehensive understanding of students’ life satisfaction.
Table 4.3: Factor-Wise Distribution of Items of Multidimensional Students’ Life Satisfaction Scale (MSLSS)
Sl. No. | Factors | Items |
1. | Family | 7 |
2. | Friends | 9 |
3. | School | 8 |
4. | Living Environment | 9 |
5. | Self | 7 |
Total | 40 |
The domains contain an unequal number of items. The mean score of each dimension is made comparable by normalising.
The normalisation procedure is as follows:
Normalised Mean =Mean of the item responses in a dimension (or total scale) /number of total items of the dimension (or total scale).
Therefore, the maximum score in each domain is 5, the minimum is 1, and the mid value is 3.
The mean of the scores of each dimension or total scale was interpreted as –
1.00 to 1.99 | : | Very Low |
2.00 to 2.99 | : | Low |
3.00 to 3.99 | : | High |
4.00 to 5.00 | : | Very high |
RESULTS
Results are presented in two subsections: (a) Descriptive Presentation, and (b) Gender-wise Comparative Analysis
Descriptive Presentation
Here, the results are presented to prove the following hypotheses:
H1: The school-going adolescents, considering both male and female as a whole, have high Academic Life Satisfaction.
Table 5.1 exhibits the descriptive statistics of scores on different facets and as a total of the Multidimensional Students Life Satisfaction (MSLSS) of the school-going adolescents in the present study.
Table 5.1: Descriptive Statistics of Scores of Different Dimensions of Multidimensional Students’ Life Satisfaction Scale (MSLSS) of the School-Going Adolescents, Considering Both Male and Female as a Whole
Life Satisfaction | N | Range | Min. | Max. | Mean | Std. Deviation | Remark |
Family | 500 | 3.43 | 1.57 | 5.00 | 4.53 | 0.80 | Very High |
Friends | 500 | 3.00 | 1.78 | 4.78 | 3.45 | 0.48 | High |
School | 500 | 3.25 | 1.75 | 5.00 | 3.46 | 0.54 | High |
Living Environment | 500 | 2.77 | 1.56 | 4.33 | 2.93 | 0.45 | Low |
Self | 500 | 3.71 | 1.29 | 5.00 | 3.45 | 0.61 | High |
MSLS in Totality | 500 | 2.35 | 2.28 | 4.63 | 3.53 | 0.41 | High |
Table 5.1 depicts the descriptive statistics of the different dimensions and the sum total of all the dimensions of Multidimensional Students’ Life Satisfaction (MSLSS) scores of the school-going adolescents. In case of the dimension related to satisfaction with family life, the “minimum” of the scores was 1.57 and the “maximum” of the scores was 5.00, and the range was 3.43; the “mean” and “standard deviation” of the said distribution were 4.53 and 0.80, respectively. Next, in case of the dimension related to satisfaction of having friends, the “minimum” of the scores was 1.78 and the “maximum” of the scores was 4.78, and the range was 3.00; the “mean” and “standard deviation” of the said distribution were 3.45 and 0.48, respectively. Then, in case of the dimension related to satisfaction of having the school, the “minimum” of the scores was 1.75 and the “maximum” of the scores was 5.00, and the range was 3.25; the “mean” and “standard deviation” of the said distribution were 3.46 and 0.54, respectively. Then, in the case of the dimension related to satisfaction derived from living environment, the “minimum” of the scores was 1.56 and the “maximum” of the scores was 4.33, and the range was 2.77; the “mean” and “standard deviation” of the said distribution were 2.93 and 0.45, respectively. Next, in case of the dimension related to satisfaction derived from self, the “minimum” of the scores was 1.29 and the “maximum” of the scores was 5.00, and the range was 3.71; the “mean” and “standard deviation” of the said distribution were 3.45 and 0.61, respectively. Finally, in case of MSLSS scores in totality, the “minimum” of the scores was 2.28 and the “maximum” of the scores was 4.63, and the range was 2.35; the “mean” and “standard deviation” of the said distribution were 3.53 and 0.41, respectively.
Figure 5.1 (a) depicts the histogram with a normal curve of Multidimensional Students Life Satisfaction (MSLSS) Scores of the school-going adolescents (considering both male and female as a whole). By visually examining, we come to know that the said distribution was about normal (Fein, Gilmour, Machin and Hendry, 2022).
Figure 5.1 (a): Histogram with normal curve of Multidimensional Students Life Satisfaction Scale (MSLSS) Scores of the School-going Adolescents Considering Both Male and Female as a Whole
Figure 5.1 (b) depicts the bar diagram of different facets of the Multidimensional Students Life Satisfaction Scale (MSLSS) Score of the school-going adolescents, considering both male and female as a whole.
Figure 5.1 (b): Bar Diagram of Different Facets of Multidimensional Students’ Life Satisfaction Scale (MSLSS) Score of the School-going Adolescents Considering Both Male and Female as a Whole
Comparative Analysis
Here, the gender-wise (i.e., male and female school-going adolescents) comparison was made. To prove the hypothesis H2[The male and female school-going adolescents do not differ with respect to their Academic Life Satisfaction.],a comparison of the two groups, here an independent sample t-test was done.
Here, the result of the gender-wise comparative analysis for academic life satisfaction is placed.
Table 5.2 (a): Group Statistics of Scores on Different Dimensionsof Multidimensional Students’ Life Satisfaction Scale (MSLSS) of the male school-going adolescents and the female school-going adolescents
Life Satisfaction | Type | N | Mean | Std. Deviation | Remarks |
Family | Male | 197 | 4.34 | 0.93 | Very High |
Female | 303 | 4.66 | 0.67 | Very High | |
Friends | Male | 197 | 3.38 | 0.52 | High |
Female | 303 | 3.50 | 0.45 | High | |
School | Male | 197 | 3.36 | 0.52 | High |
Female | 303 | 3.53 | 0.54 | High | |
Living Environment | Male | 197 | 2.89 | 0.49 | Low |
Female | 303 | 2.96 | 0.43 | Low | |
Self | Male | 197 | 3.38 | 0.63 | High |
Female | 303 | 3.50 | 0.58 | High | |
MSLS in Totality | Male | 197 | 3.43 | 0.45 | High |
Female | 303 | 3.59 | 0.36 | High |
Table 5.2 (a) shows group statistics of the scores of different dimensions of the Multidimensional Students Life Satisfaction Scale (MSLSS) of the male school-going adolescents and the female school-going adolescents. In case of satisfaction derived from Family, the means of male and female were 4.34and 4.66, respectively; again, the standard deviations were 0.93 and 0.67, respectively. Next, in case of satisfaction derived from Friends, the means of male and female were 3.38and 3.50, respectively; again, the standard deviations were 0.52and 0.45, respectively. Then, in case of satisfaction derived from School, the means of male and female were 3.36and 3.53, respectively; again, the standard deviations were 0.52and 0.54, respectively. Next, in case of satisfaction derived from Living Environment, the means of male and female adolescents were 2.89and 2.96, respectively; again, the standard deviations were 0.49 and 0.43, respectively. Then, in case of satisfaction derived from Self, the means of male and female were 3.38and 3.50, respectively; again, the standard deviations were 0.63and 0.58, respectively. Finally, in the case of Multidimensional Students’ Life Satisfaction in totality, the means of male and female were 3.43and 3.59, respectively; again, the standard deviations were 0.45 and 0.36, respectively.
Figure 5.2 shows the bar diagram of the means of scores of different dimensions of the Multidimensional Students Life Satisfaction Scale (MSLSS) of the male school-going adolescents and the female school-going adolescents.
Figure 5.2: Bar Diagram of Means of the scores in Different Dimensions of Multidimensional Students’ Life Satisfaction Scale (MSLSS) Scores of the male school-going adolescents and the female school-going adolescents.
Table 5.2 (b): Results of the Independent Samples Test of Gender Wise Comparison of Means of Scores of Different Dimensions of the Multidimensional Students Life Satisfaction Scale (MSLSS) of the male school-going adolescents and the female school-going adolescents
Life Satisfaction |
|
Levene’s Test for Equality of Variances | t-test for Equality of Means | |||
F | Sig. | t | df | Sig. (2-tailed) | ||
Family | Equal variances assumed | 27.18 | 0.00 | -4.52 | 497.00 | 0.00 |
Equal variances not assumed. | -4.23 | 328.64 | 0.00 | |||
Friends | Equal variances assumed | 4.09 | 0.04 | -2.71 | 497.00 | 0.01 |
Equal variances not assumed. | -2.63 | 376.36 | 0.01 | |||
School | Equal variances assumed | 0.35 | 0.56 | -3.56 | 497.00 | 0.00 |
Equal variances not assumed. | -3.58 | 428.98 | 0.00 | |||
Living Environment | Equal variances assumed | 1.03 | 0.31 | -1.73 | 497.00 | 0.08 |
Equal variances not assumed. | -1.68 | 376.40 | 0.09 | |||
Self | Equal variances assumed | 0.36 | 0.55 | -2.21 | 497.00 | 0.03 |
Equal variances not assumed. | -2.17 | 395.16 | 0.03 | |||
MSLS in Totality |
Equal variances assumed | 11.71 | 0.00 | -4.24 | 497.00 | 0.00 |
Equal variances not assumed. | -4.04 | 353.35 | 0.00 |
From table 5.2 (b), it is observed that the two groups (male & female) differed (statistically) significantly in multidimensional students’ life satisfaction score in totality and four dimensions of Multidimensional Students Life Satisfaction Scale (MSLSS), except the dimension related to Living Environment.
DISCUSSION
The results in Table 5.1 indicated that, on average, school-going adolescents in Paschim Medinipur District experienced high levels of life satisfaction across various domains, leading to a strong overall academic life satisfaction. Hence, hypothesis H1 was accepted. Exceptionally high family life satisfaction suggested that nurturing and supportive family relationships played a key role in promoting both emotional well-being and academic success. Likewise, high satisfaction with friendships highlighted the value of positive peer connections in fostering emotional security, recreation, and a sense of belonging. Elevated school life satisfaction reflected the benefits of engaging in academic activities, supportive teacher-student relationships, and an inclusive school environment in enriching their educational experiences. Additionally, high self-satisfaction demonstrated that adolescents maintained a strong self-concept, confidence in their abilities, and high self-esteem, which reinforced their academic motivation and success. However, lower satisfaction with the living environment pointed to concerns regarding safety, inclusivity, or recreational opportunities, indicating areas needing improvement. Nevertheless, the overall high satisfaction levels in other domains contributed to a positive academic life satisfaction among these adolescents.
Table 5.2 (a) presents the group statistics for ‘Multidimensional Students Life Satisfaction Scale (MSLSS)’ scores among male and female school-going adolescents. Table 5.2 (b) clearly indicates that there is a statistically significant difference between the two groups in the facets of Family, Friends, School, Self, and overall MSLSS scores. However, no significant difference was observed in the Living Environment dimension. On average, female school-going adolescents reported significantly higher life satisfaction in the areas of Family, Friends, School, Self, and overall life satisfaction compared to their male counterparts. Hence, the Null hypothesis H2 was rejected. The findings indicated that female adolescents in Paschim Medinipur were more likely to develop positive relationships with family members, friends, and teachers, contributing to their greater life satisfaction. Their higher family life satisfaction could be attributed to stronger familial bonds and supportive interactions, fostering mutual understanding within the household. In the friends’ domain, female adolescents likely experienced more meaningful and supportive peer relationships, enhancing their emotional well-being. Regarding school life satisfaction, they may have benefited from more positive teacher-student interactions, a stronger sense of belonging, and greater participation in school activities, all of which contributed to their higher scores. Furthermore, their elevated self-satisfaction suggested a more positive self-concept and higher self-esteem, both key predictors of life satisfaction. In contrast, male adolescents reported lower satisfaction in these areas, possibly reflecting greater challenges and stressors in their daily lives. However, the absence of a significant difference between males and females in the living environment dimension suggested that both groups were equally satisfied with their living conditions, likely due to their shared socio-economic background in Paschim Medinipur.
Therefore, hypothesis H2 was rejected.
CONCLUSION
From the result and subsequent discussion of the Descriptive Presentation, it might be concluded that the school-going adolescents of Paschim Medinipur District, considering both genders (male and female) as a whole, experienced high life satisfaction in family, friends, school, self-facets of MSLSS and also in academic life satisfaction (in totality).
From the results and subsequent discussions of the Gender-wise Comparative Analysis of the present study, it might be concluded that, on average, female school-going adolescents expressed higher academic life satisfaction than their male counterparts.
REFERENCES
- Bronfenbrenner, U. (1979).The ecology of human development: Experiments by nature and design. Harvard University Press.
- Char, S. K., Karmakar, P., Saha, B., & Adhikari, S. (2023).A Descriptive Survey on the Life Satisfaction of the School-Going Adolescents of Purulia District. International Journal of Research Science, 12(1), 1-3.
- Clarke, T., McLellan, R. & Harold, G. (2023). Beyond Life Satisfaction: Wellbeing Correlates of Adolescents’ Academic Attainment. School Psychology Review, (), 1-20. https://doi.org/10.1080/2372966X.2023.2217980
- Diener, E. (2000). Subjective well-being: The science of happiness and a proposal for a national index. American Psychologist, 55, 34-43. https://doi.org/10.1037/0003-066X.55.1.34
- Eccles, J. S., & Roeser, R. W. (2011).Schools as developmental contexts during adolescence. Journal of Research on Adolescence, 21(1), 225–241. https://doi.org/10.1111/j.1532-7795.2010.00725.x
- Fein, E.C., Gilmour, J., Machin, T., & Hendry, L. (2022). Statistics for Research Students: an Open Access Resource with Self-Tests and Illustrative Examples. University of Southern Queensland land Toowoomba.
- Gallego, A. G., Pérez, A. M., Fernández, V. F., Alcántara-López, M., & Sáez, M. C. (2021).Life satisfaction in adolescents: relationship with parental style, peer attachment and emotional intelligence. Electronic Journal of Research in Educational Psychology, 19(53), 51-74. https://ojs.ual.es/ojs/index.php/EJREP/article/download/3425/4542/15183
- Huebner, E. S. (1994). Preliminary development and validation of a multidimensional life satisfaction scale for children. Psychological Assessment, 6, 149–158. http://dx.doi.org/10.1037/1040-3590.6.2.149
- Huebner, E. S., Gilman, R., & Laughlin, J. E. (1999). A multimethod investigation of the multidimensionality of children’s well-being reports: Discriminant validity of life satisfaction and self-esteem. Social Indicators Research, 46(1), 1–22. https://doi.org/10.1023/A:1006821510832
- Huebner, E. S., Laughlin, J. E., Ash, C., & Gilman, R. (1998). Further validation of the multidimensional students’ life satisfaction scale. Journal of Psychoeducational Assessment, 16(2), 118-134. http://dx.doi.org/10.1177/073428299801600202
- Lábiscsák-Erdélyi, Z., Veres-Balajti, I., Somhegyi, A., & Kósa, K. (2022).Self-Esteem Is an Independent Factor and Moderator of School-Related Psychosocial Determinants of Life Satisfaction in Adolescents. International Journal of Environmental Research and Public Health, 19(9), 55-65. https://doi.org/10.3390/ijerph19095565
- Manna, N., Mullick, T. H., Mazumdar, S. D., & Das, S. (2023). A study on self-esteem and life satisfaction among adolescents aged 14–19 years attending an adolescent-friendly health clinic, Medical College, Kolkata. National Journal of Physiology, Pharmacy and Pharmacology, 13(11), 2241-2247. https://njppp.com/fulltext/28-1694793299.pdf?1710138138
- Mistry, S., Halder, M., Karmakar, M., Mukherjee, S., & Adhikari, S. (2024). A probe into the academic life satisfaction of the school-going adolescents. International Journal of Social Science Education and Research, 6(1), 43-50. https://doi.org/10.33545/26649845.2024.v6.i1a.80
- Palacios, E. G., Echaniz, I. E., Fernández, A. R., & Barrón, I.C.O. (2015). Personal self-concept and satisfaction with life in adolescence, youth and adulthood. Psicothema, 27(1), 52-58. https://doi.org/10.7334/psicothema2014.105
- (2024). Effect of Self-Esteem on Life Satisfaction Among Adolescents. International Journal of Multidisciplinary Research, 10(1), 665-669. https://doi.org/10.36713/epra15693
- Ryan, R. M., & Deci, E. L. (2000). Intrinsic and Extrinsic Motivations: Classic definitions and new directions. Contemporary Educational Psychology,25, 54-67.https://doi.org/10.1006/ceps.1999.1020
- Saad, J., Saad, M.N., & Sahibzada, A. (2020). Self-Esteem and Life Satisfaction amongst Afghan and Indian University Students. International Journal of Creative Research Thoughts. 8(7), 2824-2829. http://dx.doi.org/10.6084/m9.doi.one.IJCRT2007271
- Šimunović, Z. & Olčar, D. (2022). Life Satisfaction among Adolescents: Comparison of Adolescents Attending Music and Sports Programs and Those Who Do Not. International Journal of Learning, Teaching and Educational Research, 21(5), 428-445. https://doi.org/10.26803/ijlter.21.5.21
- Steinberg, L. (2014).Adolescence (10th ed.). McGraw-Hill Education.
- Supervía, P. U., Bordás, C. S., Basterretxea, J. J., & Cosculluela, C. L. (2023).Influence of Psychological Variables in Adolescence: The Mediating Role of Self-Esteem in the Relationship between Self-Efficacy and Satisfaction with Life in Senior High School Students. Social Sciences, 12(6), 1-12. https://doi.org/10.3390/socsci12060329
- Szcze´sniak, M., Bajkowska, I., Czaprowska, A. & Sile ´nska, A. (2022). Adolescents’ Self-Esteem and Life Satisfaction: Communication with Peers as a Mediator. International Journal of Environmental Research and Public Health. https://doi.org/10.3390/ijerph19073777
- Zuo, Z., Li, S., Liu, S., & Wang, Q. (2022).Life satisfaction and parental support among secondary school students in Urumqi: the mediation of physical activity. Peer J, 10 (e14122). http://doi.org/10.7717/peerj.14122