Effects of Separate and Combined Chronic Ingestion of Alcohol and Energy Drinks on Aggressive Behaviour in Female Albino Wistar Rats

Aggressive behaviour is a complex neurobehavioural phenomenon influenced by biological, psychological, and environmental factors, including exposure to psychoactive substances. Although alcohol and energy drinks have each been independently linked to alterations in aggression, limited experimental evidence exists regarding their separate and combined effects, particularly in female animal models. This study investigated the chronic effects of alcohol, energy drinks, and their co-administration on aggressive behaviour in female albino Wistar rats. A total of 28 female albino Wistar rats were randomly assigned to four groups: Control, Energy Drink, Alcohol, and Alcohol combined with Energy Drink. Rats received daily oral administration of alcohol (0.5 ml/kg body weight), energy drink (1.0 ml/kg body weight), or their combination for 28 consecutive days. Aggressive behaviour was assessed using the validated Resident–Intruder Paradigm, with dominant posture, scratching, and biting recorded as indices of aggression. Data were analysed using One-Way ANOVA with Bonferroni post hoc comparisons. Results revealed a statistically significant effect of treatment on aggressive behaviour, p < .001, with a large effect size (partial η² ≈ .87). Rats exposed to energy drinks exhibited the highest levels of aggression (M = 76.00), followed by alcohol-only exposure (M = 62.43), while the combined alcohol and energy drink group showed the lowest aggression (M = 34.57), even below control levels (M = 44.86). The findings indicate that chronic exposure to alcohol and energy drinks significantly modulates aggressive behaviour in female albino Wistar rats, with stimulatory and depressant substances producing distinct and interactive effects.

Aggression, Alcohol, Energy Drinks

1. Aleyasin, H., Flanigan, M. E., & Russo, S. J. (2018). Neurocircuitry of aggression and aggression-seeking behavior: Nose poking into brain circuitry controlling aggression. Current Opinion in Neurobiology, 49, 184–191. [Google Scholar] [Crossref]

2. Anderson, C. A., & Bushman, B. J. (2002). Human aggression. Annual Review of Psychology, 53, 27–51. https://doi.org/10.1146/annurev.psych.53.100901.135231 [Google Scholar] [Crossref]

3. Anderson, C. A., & Bushman, B. J. (2018). The general aggression model. Current Opinion in Psychology, 19, 75–80. https://doi.org/10.1016/j.copsyc.2017.03.034 [Google Scholar] [Crossref]

4. Balogun, S. K. (2006). Chronic intake of separate and combined alcohol and nicotine on body maintenance among albino rats. Journal of Human Ecology, 19(1), 21–24. [Google Scholar] [Crossref]

5. Balogun, S. K., Osuh, J. I., & Olorede, G. (2020). Effects of separate and combined chronic ingestion of tramadol and codeine on aggressive behaviour among female albino rats. Covenant International Journal of Psychology, 5(1). [Google Scholar] [Crossref]

6. Bandura, A. (1973). Aggression: A social learning analysis. Prentice-Hall. [Google Scholar] [Crossref]

7. Brache, K., & Stockwell, T. (2011). Drinking patterns and risk behaviors associated with combined alcohol and energy drink consumption in college drinkers. Addictive Behaviors, 36(12), 1133–1140. https://doi.org/10.1016/j.addbeh.2011.07.003 [Google Scholar] [Crossref]

8. Costantino, A., Maiese, A., Lazzari, J., Casula, C., Turillazzi, E., Frati, P., & Fineschi, V. (2023). The dark side of energy drinks: A comprehensive review of their impact on the human body. Nutrients, 15(18), 3922. https://doi.org/10.3390/nu15183922 [Google Scholar] [Crossref]

9. Drndarević, N. (2021). Psychological theories of aggression. Zbornik Instituta za Kriminološka i Sociološka Istraživanja, 40(2–3), 91–104. https://doi.org/10.47152/ziksi202123026 [Google Scholar] [Crossref]

10. Elvig, S. K., McGinn, M. A., Smith, C., Arends, M. A., Koob, G. F., & Vendruscolo, L. F. (2021). Tolerance to alcohol: A critical yet understudied factor in alcohol addiction. Pharmacology, Biochemistry and Behavior, 204, 173155. https://doi.org/10.1016/j.pbb.2021.173155 [Google Scholar] [Crossref]

11. Figdor, C. (2022). Animal models in neuropsychiatry: Do the benefits outweigh the moral costs? Cambridge Quarterly of Healthcare Ethics, 31(4), 530–535. https://doi.org/10.1017/S0963180122000147 [Google Scholar] [Crossref]

12. Girasek, H., Nagy, V. A., Fekete, S., Ungvari, G. S., & Gazdag, G. (2022). Prevalence and correlates of aggressive behavior in psychiatric inpatient populations. World Journal of Psychiatry, 12(1), 1–23. https://doi.org/10.5498/wjp.v12.i1.1 [Google Scholar] [Crossref]

13. Heinz, A., Beck, A., & Meyer-Lindenberg, A. (2020). Cognitive and neurobiological mechanisms of alcohol-induced aggression. Nature Reviews Neuroscience, 21, 608–624. [Google Scholar] [Crossref]

14. Heinz, A. J., de Wit, H., Lilje, T. C., & Kassel, J. D. (2013). The combined effects of alcohol, caffeine, and expectancies on subjective experience, impulsivity, and risk-taking. Alcoholism: Clinical and Experimental Research, 37(3), 509–516. https://doi.org/10.1111/j.1530-0277.2012.01948.x [Google Scholar] [Crossref]

15. Hoaken, P. N. S., & Stewart, S. H. (2003). Drugs of abuse and the elicitation of human aggressive behavior. Addictive Behaviors, 28(9), 1533–1554. https://doi.org/10.1016/j.addbeh.2003.08.033 [Google Scholar] [Crossref]

16. Huesmann, L. R. (2017). An integrative theoretical understanding of aggression. In B. J. Bushman (Ed.), Aggression and violence: A social psychological perspective (pp. 3–21). Routledge/Taylor & Francis Group. [Google Scholar] [Crossref]

17. Juliano, L. M., & Griffiths, R. R. (2004). A critical review of caffeine withdrawal: Empirical validation of symptoms and signs, incidence, severity, and associated features. Psychopharmacology, 176(1), 1–29. https://doi.org/10.1007/s00213-004-2000-x [Google Scholar] [Crossref]

18. Kolla, N. J., & Kennedy, J. L. (2017). Are we there yet? A neurobiological model of violence and aggression. Journal of Psychiatry & Neuroscience, 39(3), 133–135. [Google Scholar] [Crossref]

19. Liu, J., Lewis, G., & Evans, L. (2013). Understanding aggressive behaviour across the lifespan. Journal of Psychiatric and Mental Health Nursing, 20, 156–168. [Google Scholar] [Crossref]

20. Mitchell, G., & McCambridge, J. (2023). Interactions between the U.S. National Institute on Alcohol Abuse and Alcoholism and the alcohol industry: Evidence from email correspondence 2013–2020. Journal of Studies on Alcohol and Drugs, 84(1), 11–26. https://doi.org/10.15288/jsad.22-00184 [Google Scholar] [Crossref]

21. Natarajan, D., & Caramaschi, D. (2010). Animal violence demystified. Frontiers in Behavioral Neuroscience, 4, Article 9. https://doi.org/10.3389/fnbeh.2010.00009 [Google Scholar] [Crossref]

22. Nawi, A. M., Ismail, R., Ibrahim, F., Hassan, M. R., Manaf, M. R. A., Amit, N., Ibrahim, N., & Shafurdin, N. S. (2021). Risk and protective factors of drug abuse among adolescents: A systematic review. BMC Public Health, 21, Article 2088. https://doi.org/10.1186/s12889-021-11906-2 [Google Scholar] [Crossref]

23. Oscar-Berman, M., & Marinković, K. (2007). Alcohol: Effects on neurobehavioral functions and the brain. Neuropsychology Review, 17(3), 239–257. https://doi.org/10.1007/s11065-007-9038-6 [Google Scholar] [Crossref]

24. Seifert, S. M., Schaechter, J. L., Hershorin, E. R., & Lipshultz, S. E. (2011). Health effects of energy drinks on children, adolescents, and young adults. Pediatrics, 127(3), 511–528. https://doi.org/10.1542/peds.2010-3502 [Google Scholar] [Crossref]

25. World Health Organization. (2024, June 19). Injuries and violence. [Google Scholar] [Crossref]

26. Zeidán-Chuliá, F., de Oliveira, B. H., Salmina, A. B., Casanova, M. F., & Verkhratsky, A. (2014). Altered expression of Alzheimer’s disease–related genes in the cerebellum of autistic patients: A model for disrupted brain connectome and therapy. Cell Death & Disease, 5, e1250. https://doi.org/10.1038/cddis.2014.227 [Google Scholar] [Crossref]

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