In Vitro Evaluation of the Nematicidal Efficacy of Selected Plant Leaf Extracts against Potato Cyst Nematodes (Globodera spp.)

Potato cyst nematodes (Globodera spp.) are among the most destructive pests affecting potato production, causing significant yield losses worldwide. The increasing concerns over the environmental and health impacts of synthetic nematicides have prompted the search for sustainable and eco-friendly alternatives. This study aimed to evaluate the nematicidal efficacy of selected plant leaf extracts under in vitro conditions, focusing on their effects on egg hatching, cyst count, and second-stage juvenile (J2) mortality. Aqueous leaf extracts of neem (Azadirachta indica), madre de cacao (Gliricidia sepium), mahogany (Swietenia macrophylla), hagonoy (Chromolaena odorata), and papaya (Carica papaya) were prepared and tested using a completely randomized design with three replications.
Results showed that all plant extracts exhibited varying degrees of nematicidal activity. Among the treatments, Treatment 1 demonstrated the highest efficacy, significantly reducing egg hatching compared to the control, indicating strong ovicidal properties. Moderate effects were observed in other treatments, while one treatment showed minimal inhibitory activity. In contrast, the impact of the extracts on juvenile mortality was less pronounced, with only slight variations in juvenile counts across treatments. This suggests that the plant extracts are more effective in inhibiting egg development than in killing already emerged juveniles.

Potato Cyst Nematodes, Nematicides, Juvenile stage, plant leaf extract

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