The Acceptability of Bignay (Antidesma Bunius) Fruit Extract as an Organic-Based pH Indicator
Authors
Degree of Bachelor of Secondary Education Major in Science Student Researcher and Faculty of the Laguna University, Sta. Cruz, Laguna (Philippines)
Degree of Bachelor of Secondary Education Major in Science Student Researcher and Faculty of the Laguna University, Sta. Cruz, Laguna (Philippines)
Degree of Bachelor of Secondary Education Major in Science Student Researcher and Faculty of the Laguna University, Sta. Cruz, Laguna (Philippines)
Degree of Bachelor of Secondary Education Major in Science Student Researcher and Faculty of the Laguna University, Sta. Cruz, Laguna (Philippines)
Degree of Bachelor of Secondary Education Major in Science Student Researcher and Faculty of the Laguna University, Sta. Cruz, Laguna (Philippines)
Article Information
DOI: 10.47772/IJRISS.2026.100300121
Subject Category: Chemistry
Volume/Issue: 10/3 | Page No: 1744-1761
Publication Timeline
Submitted: 2026-03-11
Accepted: 2026-03-19
Published: 2026-03-28
Abstract
Nowadays, experts are actively seeking ways to become more eco-friendly in response to the pressing environmental challenges. Similarly, educational institutions are encouraged to implement sustainable practices by promoting the use of environmentally safe materials in scientific activities. The goal of this study was to assess the acceptability of Bignay pH indicator as an alternative to synthetic pH indicators in school laboratories. To achieve this, the study employed an Explanatory Sequential Research Design, which combines both Experimental and Descriptive approaches. To obtain the necessary data, the researchers conducted controlled experiments, in-depth interviews with Professional Chemistry Teachers using structured survey questionnaires along with live demonstrations, and the product underwent a phytochemical laboratory test by DOST. Upon analyzing the results, it was revealed that Bignay extract showed potential as a natural pH indicator. This claim was strengthened by the DOST phytochemical test; the test report revealed that Bignay fruit extract pH indicator contained significant constituents such as Sterols, Triterpenes, Flavonoids, Alkaloids, Glycosides, and Tannins, which were known to have color-changing properties in response to varying pH levels. Hence, the extract proved to be suitable for use as a natural pH indicator. Moreover, the acceptability phase garnered a mean of >3.50 for parameters such as Color, Odor, and Accuracy, which unveiled that the Bignay pH indicator was an acceptable alternative to synthetic indicators used in schools its sharp color transitions ranging from pink, green, blue, and yellow, its subtle odor, and its accuracy in determining the pH level of substances. In addition, the shelf life extended up to sixty (60) days making it appropriate for use in laboratory activities. To obtain more accurate results, the researchers recommend the use of fully ripened (violet-colored) Bignay (Antidesma bunius) fruits.
Keywords
Bignay, Acceptability, pH indicator, Phytochemical, Shelf life
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References
1. Aksornchu, P., Chamnansilpa, N., Adisakwattana, S., Thilavech, T., Choosak, C., Marnpae, M., Mäkynen, K., Dahlan, W., & Ngamukote, S. (2020). Inhibitory Effect of Antidesma bunius Fruit Extract on Carbohydrate Digestive Enzymes Activity and Protein Glycation In Vitro. Antioxidants, 10(1), 32. https://doi.org/10.3390/antiox10010032 [Google Scholar] [Crossref]
2. Baladlad, M.E.B., Culaton-Milan, J. V., Reyes, A. B.(2022). Red dragon fruit (Hylocereus costaricensis) flesh aqueous extract as a natural pH indicator. The Lormanian Microscopists. https://www.researchgate.net/publication/371733401_Red_dragon_fruit_Hylocereus_costaricensis_flesh_aqueous_extract_as_a_natural_pH_indicator [Google Scholar] [Crossref]
3. Bashyal, J. (2022, December 17). PH - Definition, Calculation, and Significance. Science Info. https://scienceinfo.com/ph/ [Google Scholar] [Crossref]
4. Bertelli, A., Biagi, M., Corsini, M., Baini, G., Cappellucci, G., & Miraldi, E. (2021). Polyphenols: From Theory to practice. Foods, 10(11), 2595. https://doi.org/10.3390/foods10112595 [Google Scholar] [Crossref]
5. Carbonera, A. F. A., Atienza, L. M., Estacio, M. a. C., Duque, S. M. M., Lizardo-Agustin, R. C. M., Flandez, L. E. L., & Castillo-Israel, K. a. T. (2023b). Effects of various processing methods on the dietary fiber and antioxidant properties of Bignay (Antidesma bunius L. Spreng) fruit. Food Chemistry Advances, 3, 100561. https://doi.org/10.1016/j.focha.2023.100561 [Google Scholar] [Crossref]
6. Crieta, B. R. A., Tuaño, A. P. P., Torio, M. a. O., Villanueva, J. C., Gaban, P. J. V., & Castillo-Israel, K. a. T. (2021). In vitro lipid-lowering properties of the fruits of two bignay [Antidesma bunius (L.) Spreng] cultivars as affected by maturity stage and thermal processing. Food Chemistry Molecular Sciences, 2, 100020. https://doi.org/10.1016/j.fochms.2021.100020 [Google Scholar] [Crossref]
7. Dara, S. R. (2024b). An overview of the use of natural indicators in Acid-Base titrations. UPI Journal of Pharmaceutical Medical and Health Sciences, 29–35. https://doi.org/10.37022/jpmhs.v4i2.103 [Google Scholar] [Crossref]
8. Emily, H., Gabriela, H. H. N., & Jorge, B. (2019d). OBTAINING AN ACID-BASE NATURAL INDICATOR FROM THE FRUIT Punica granatum l. (POMEGRANATE): a CONTRIBUTION FOR THE SUBSTITUTION OF SYNTHETIC INDICATORS. Journal of the Chilean Chemical Society, 64(4), 4593–4596. https://doi.org/10.4067/s0717-97072019000404593 [Google Scholar] [Crossref]
9. Fuentes, P. L. S., Legaspi, J. S., & Albaladejo, V. E. (2021b). Phyllanthus (Phyllanthus multiflorus Willd.) Fruit as Natural pH Indicator. International Journal of Advanced Engineering Research and Science, 8(10), 335–339. https://doi.org/10.22161/ijaers.810.35 [Google Scholar] [Crossref]
10. Helmenstine, A. (2021, August 16). What is an aqueous solution? Definition and examples. Science Notes and Projects. https://sciencenotes.org/what-is-an-aqueous-solution-definition-and-examples/ [Google Scholar] [Crossref]
11. Hemdan, S. S., Jebaly, A. M. A., & Ali, F. K. (2024). The behaviour and properties of some acid-base indicators: a review. The Behavior and Properties of Some Acid-base Indicators: A Review, 66, 1–22. https://doi.org/10.37376/jsh.vi66.5615 [Google Scholar] [Crossref]
12. Jabeen, M. and Tariq, K. et.al (2022). Evaluation of Improvised and Eco-Friendly Natural pH-Paper Indicators. https://pdfs.semanticscholar.org/b7d6/e34208b3c9de1627139ae8ab1b0dcea3f2e8.pdf [Google Scholar] [Crossref]
13. Nag, M., Paul, R. K., Biswas, S., Dasgupta, D., Roy, D., Bhattacharjee, P., Chattopadhyay, S., & Mallick, A. (2023). A review on Application of natural indicators in acid-base titration. Pharmacognosy Reviews/Bioinformatics Trends/Pharmacognosy Review, 17(34), 308–319. https://doi.org/10.5530/phrev.2023.17.10 [Google Scholar] [Crossref]
14. Pomelo. (2022, November 3). Why is pH important? | Atlas Scientific. Atlas Scientific. [Google Scholar] [Crossref]
15. https://atlas-scientific.com/blog/why-is-ph-important/ [Google Scholar] [Crossref]
16. Romford, L. (2024, January 3). What is indicator in Chemistry. AcademicHelp.net. https://academichelp.net/stem/chemistry/what-is-indicator.html [Google Scholar] [Crossref]
17. Roy, S., & Rhim, J. (2020b). Anthocyanin food colorant and its application in pH-responsive color change indicator films. Critical Reviews in Food Science and Nutrition, 61(14), 2297–2325. https://doi.org/10.1080/10408398.2020.1776211 [Google Scholar] [Crossref]
18. Shenekji, J., Shayah, K., Karkoukli, N., Sulaiman, Y., Nerabi, N., & Shayyah, B. (2025b). Preparing an Eco-Friendly pH indicator using red cabbage extract suitable for Low-Budget Labs V1. https://doi.org/10.17504/protocols.io.yxmvmmre5v3p/v1 [Google Scholar] [Crossref]
19. Sohany, M., Tawakkal, I. S. M. A., Ariffin, S. H., Shah, N. N. a. K., & Yusof, Y. A. (2021). Characterization of Anthocyanin Associated Purple Sweet potato Starch and Peel-Based pH indicator films. Foods, 10(9), 2005. https://doi.org/10.3390/foods10092005 [Google Scholar] [Crossref]
20. Xiao, Z., Han, L., Gu, M., Zhu, Y., Zhang, Y., Li, Z., Xie, T., & Lu, F. (2023). Performance comparison of anthocyanin-based smart indicator films. Food Packaging and Shelf Life, 40, 101187. https://doi.org/10.1016/j.fpsl.2023.101187 [Google Scholar] [Crossref]
21. Xue, H., Zhao, J., Wang, Y., Shi, Z., Xie, K., Liao, X., & Tan, J. (2024). Factors affecting the stability of anthocyanins and strategies for improving their stability: A review. Food Chemistry X, 24, 101883. https://doi.org/10.1016/j.fochx.2024.101883 [Google Scholar] [Crossref]
22. Zhao, L., Liu, Y., Zhao, L., & Wang, Y. (2022). Anthocyanin-based pH-sensitive smart packaging films for monitoring food freshness. Journal of Agriculture and Food Research, 9, 100340. https://doi.org/10.1016/j.jafr.2022.100340 [Google Scholar] [Crossref]
23. Zubia, C. S., Babaran, G. M. O., Duque, S. M. M., Mopera, L. E., Flandez, L. E. L., Castillo-Israel, K. a. T., & Reginio, F. C. (2023). Impact of drying on the bioactive compounds and antioxidant properties of bignay [Antidesma bunius (L.) Spreng.] pomace. Food Production Processing and Nutrition, 5(1). https://doi.org/10.1186/s43014-022-00122- [Google Scholar] [Crossref]
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