Antimicrobial Resistance of Staphylococcus Aureus in The Dairy Industry: A One Health Concern

The global dairy industry faces a persistent and escalating challenge from Staphylococcus aureus, a primary agent of bovine mastitis and a major public health pathogen. This review synthesizes evidence to examine the complex epidemiology of antimicrobial resistance (AMR) in dairy-associated S. aureus, a crisis propelled by antimicrobial misuse in livestock and facilitated by dynamic transmission at the human-animal-environment interface. Studies from Africa, Asia, Europe, and America reveal a high and geographically variable prevalence of multidrug-resistant (MDR) and methicillin-resistant S. aureus (MRSA) strains, with particularly concerning resistance to beta-lactams and tetracyclines. Molecular analyses highlight the adaptation of successful clonal complexes (e.g., CC97, ST-1) in dairy herds and the widespread carriage of enterotoxin genes, resulting in zoonotic and food safety risks independent of AMR status. The transmission of resistant strains is amplified by suboptimal farm hygiene, direct occupational contact, and environmental contamination, with raw milk serving as a key vehicle for human exposure. The convergence of MDR, methicillin resistance, and potent virulence determinants in these strains poses a severe One Health threat, leading to difficult-to-treat infections that increase morbidity and healthcare costs. Mitigating this crisis requires an integrated, multi-pronged strategy. This includes stringent antimicrobial stewardship such as culture-guided therapy and selective dry cow treatment coupled with improved biosecurity, milking hygiene, and comprehensive surveillance. The development and adoption of alternative interventions, including vaccines, bacteriophages, and probiotics, are promising avenues to reduce antibiotic dependence. Concerted, cross-sectoral action under a One Health framework is urgently needed to curb the spread of AMR, protect animal welfare, ensure dairy sector sustainability, and safeguard global public health security.

Antimicrobial resistance; Staphylococcus aureus; mastitis; One Health; zoonosis.

1. Akindolire, M. A., Babalola, O. O., & Ateba, C. N. (2015). Detection of antibiotic resistant Staphylococcus aureus from milk: A public health implication. International Journal of Environmental Research and Public Health, 12(9), 10254–10275. [Google Scholar] [Crossref]

2. Algammal, A. M., Hetta, H. F., Elkelish, A., Alkhaliҁah, D. H. H., Hozzein, W. N., Batiha, G. E.-S., El Nahhas, N., & Mabrok, M. A. (2020). Methicillin-resistant Staphylococcus aureus (MRSA): One Health perspective approach to the bacterium epidemiology, virulence factors, antibiotic-resistance, and zoonotic impact. Infection and Drug Resistance, 13, 3255–3265. [Google Scholar] [Crossref]

3. Aqib, A. I., Ijaz, M., Farooqi, S. H., & Raza, A. (2018). Dairy Staphylococcus aureus: Epidemiology, drug susceptibilities, drug modulation, and preventive measures. In Staphylococcus aureus. IntechOpen. http://dx.doi.org/10.5772/intechopen.74552 [Google Scholar] [Crossref]

4. Aqib, A. I., Ijaz, M., Farooqi, S. H., Ahmed, R., Shoaib, M., Ali, M. M., .& Naseer, M. A. (2018). Emerging discrepancies in conventional and molecular epidemiology of methicillin resistant Staphylococcus aureus isolated from bovine milk. Microbial Pathogenesis, 116, 38–43. [Google Scholar] [Crossref]

5. Aqib, A. I., Saqib, M., Khan, S. R., Ahmad, T., Shah, S. A., Naseer, M. A., ... & Shoaib, M. (2021). Non-steroidal anti-inflammatory drugs, plant extracts, and characterized microparticles to modulate antimicrobial resistance of epidemic mecA positive S. aureus of dairy origin. Applied Nanoscience. Advance online publication. [Google Scholar] [Crossref]

6. Badawy, B., Elaffy, M., Farag, A. M. M., Moustafa, S. M., Sayed-Ahmed, M. Z., Moawad, A. A., Algammal, A. M., Ramadan, H., & Eltholth, M. (2022). Ecological distribution of virulent multidrug-resistant Staphylococcus aureus in livestock, environment, and dairy products. Antibiotics, 11(11), 1651. [Google Scholar] [Crossref]

7. Batool, S., Masood, Z., Ullah, A., Khan, W., Said, M. B., Belkahia, H., Ismael, A. B., & Swelum, A. A. (2025). Isolation of antibiotic-resistant strains of Staphylococcus aureus from raw milk produced by dairy cows with subclinical bovine mastitis. Journal of Advanced Veterinary and Animal Research, 12(1), 252–259. [Google Scholar] [Crossref]

8. Bharathy, S., Gunaseelan, L., Porteen, K., & Bojiraj, M. (2015). Prevalence of Staphylococcus aureus in raw milk: Can it be a potential public health threat? International Journal of Advanced Research, 3(2), 801–806. [Google Scholar] [Crossref]

9. Bonnedahl, J., & Järhult, J. D. (2014). Antibiotic resistance in wild birds. Upsala Journal of Medical Sciences, 119(2), 113–116. https://doi.org/10.3109/03009734.2014.905663 [Google Scholar] [Crossref]

10. Cardoso, C. V., Barbosa, E. V., Liberal, M. I. T., & das Chagas, E. F. (2019). Transgenic technology: the strategy for the control and prevention of bovine staphylococcal mastitis? Biotechnology Research and Innovation, 3(2), 291–297. [Google Scholar] [Crossref]

11. Cosgrove, S. E. (2006). The relationship between antimicrobial resistance and patient outcomes: Mortality, length of hospital stay, and health care costs. Clinical Infectious Diseases, 42(Suppl 2), S82–S89. [Google Scholar] [Crossref]

12. Côté-Gravel, J., & Malouin, F. (2019). Symposium review: Features of Staphylococcus aureus mastitis pathogenesis that guide vaccine development strategies. Journal of Dairy Science, 102(5), 4727–4740. [Google Scholar] [Crossref]

13. Dufour, S., Dohoo, I. R., Barkema, H. W., DesCôteaux, L., DeVries, T. J., Reyher, K. K., Roy, J.-P., & Scholl, D. T. (2012). Manageable risk factors associated with the lactational incidence, elimination, and prevalence of Staphylococcus aureus intramammary infections in dairy cows. Journal of Dairy Science, 95(3), 1283–1300. https://doi.org/10.3168/jds.2011-4711 [Google Scholar] [Crossref]

14. Elbehiry, A., & Marzouk, E. (2025). Staphylococci in livestock: Molecular epidemiology, antimicrobial resistance, and translational strategies for One Health protection. Veterinary Sciences, 12(8), 757. https://doi.org/10.3390/vetsci12080757 [Google Scholar] [Crossref]

15. Fan, J., Zeng, Z., Mai, K., Yang, Y., Feng, J., Bai, Y., & Ma, X. (2016). Preliminary treatment of bovine mastitis caused by Staphylococcus aureus, with trx-SA1, recombinant endolysin of S. aureus bacteriophage IME-SA1. Veterinary Microbiology, 191, 65–71. [Google Scholar] [Crossref]

16. Fang, H., Han, L., Zhang, H., Long, Z., Cai, L., & Yu, Y. (2018). Dissemination of antibiotic resistance genes and human pathogenic bacteria from a pig feedlot to the surrounding stream and agricultural soils. Journal of Hazardous Materials, 357, 53–62. https://doi.org/10.1016/j.jhazmat.2018.05.066 [Google Scholar] [Crossref]

17. Gadde, U., Kim, W. H., Oh, S. T., & Lillehoj, H. S. (2017). Alternatives to antibiotics for maximizing growth performance and feed efficiency in poultry: A review. Animal Health Research Reviews, 18(1), 26–45. [Google Scholar] [Crossref]

18. Ge, B., Zhao, P., Li, H., Sang, R., Wang, M., Zhou, H., & Zhang, X. (2020). Taraxacum mongolicum protects against Staphylococcus aureus-infected mastitis by exerting anti-inflammatory role via TLR2-NF-κB/MAPKs pathways in mice. Journal of Ethnopharmacology, 263, 113595. [Google Scholar] [Crossref]

19. Geletu, U. S., Usmael, M. A., & Ibrahim, A. M. (2022). Isolation, identification, and susceptibility profile of E. coli, Salmonella, and S. aureus in dairy farm and their public health implication in central Ethiopia. Veterinary Medicine International, 2022, Article 1887977. [Google Scholar] [Crossref]

20. Gogoi-Tiwari, J., Williams, V., & Tiwari, H. K. (2020). Methicillin-resistant Staphylococcus aureus (MRSA) in dairy animals and the need for a One Health approach. Journal of Applied Microbiology, 129(5), 1101-1114. https://doi.org/10.1111/jam.14715 [Google Scholar] [Crossref]

21. Gomes, F., Martins, N., Barros, L., Rodrigues, M. E., Oliveira, M. B. P. P., Henriques, M., & Ferreira, I. C. F. R. (2018). Plant phenolic extracts as an effective strategy to control Staphylococcus aureus, the dairy industry pathogen. Industrial Crops and Products, 112, 515–520. https://doi.org/10.1016/j.indcrop.2017.12.027 [Google Scholar] [Crossref]

22. Greening, S. S., Zhang, J., Midwinter, A. C., Wilkinson, D. A., McDougall, S., Gates, M. C., & French, N. P. (2021). The genetic relatedness and antimicrobial resistance patterns of mastitis-causing Staphylococcus aureus strains isolated from New Zealand dairy cattle. Veterinary Sciences, 8(11), 287 [Google Scholar] [Crossref]

23. Gutama, K. P., & Anberber, M. (2025). Epidemiological investigation and antimicrobial sensitivity of Staphylococcus aureus in dairy farms of selected towns in Western Oromia, Ethiopia. BMC Veterinary Research, 21(420), 1–12. [Google Scholar] [Crossref]

24. Haag, A. F., Fitzgerald, J. R., & Penadés, J. R. (2019). Staphylococcus aureus in animals. Microbiology Spectrum, 7(3). [Google Scholar] [Crossref]

25. Hagnestam-Nielsen, C., & Østergaard, S. (2009). Economic impact of clinical mastitis in a dairy herd assessed by stochastic simulation using different methods to model yield losses. Animal, 3(2), 315–328. [Google Scholar] [Crossref]

26. Hennekinne, J. A., De Buyser, M. L., & Dragacci, S. (2012). Staphylococcus aureus and its food poisoning toxins: Characterization and outbreak investigation. FEMS Microbiology Reviews, 36(4), 815–836. [Google Scholar] [Crossref]

27. Ibekwe, A. M., Bhattacharjee, A. S., Phan, D., Ashworth, D., Schmidt, M. P., Murinda, S. E., Obayiuwana, A., Murry, M. A., Schwartz, G., & Lundquist, T. (2023). Potential reservoirs of antimicrobial resistance in livestock waste and treated wastewater that can be disseminated to agricultural land. Science of The Total Environment, *872*, 162194. https://doi.org/10.1016/j.scitotenv.2023.162194 [Google Scholar] [Crossref]

28. Islam, M. M., Hossain, M. I., Islam, M. S., Azam, M. G., & Sultana, S. (2025). Prevalence, antibiotic resistance patterns, and virulence factors of Staphylococcus aureus isolates associated with bovine mastitis in northern Bangladesh. Heliyon, 11(3), e42107. [Google Scholar] [Crossref]

29. Jamali, H., Paydar, M., Radmehr, B., Ismail, S., & Dadrasnia, A. (2015). Prevalence and antimicrobial resistance of Staphylococcus aureus isolated from raw milk and dairy products. Food Control, 54, 383–388. [Google Scholar] [Crossref]

30. Landin, H., Jansson Mörk, M., Larsson, M., & Waller, K. P. (2015). Vaccination against Staphylococcus aureus mastitis in two Swedish dairy herds. Acta Veterinaria Scandinavica, 57(1), 81. [Google Scholar] [Crossref]

31. Leitner, G., Krifucks, O., Kiran, M. D., & Balaban, N. (2011). Vaccine development for the prevention of staphylococcal mastitis in dairy cows. Veterinary Immunology and Immunopathology, 142(1–2), 25–35. [Google Scholar] [Crossref]

32. Marami, L. M., Berhanu, G., Tekle, M., Agga, G. E., Beyene, T. J., Tufa, T. B., Beyi, A. F., & Edao, B. M. (2022). Antimicrobial resistance of staphylococci at animal human interface in smallholders and dairy farms in Central Oromia, Ethiopia. Infection and Drug Resistance, 15, 3767–3777. [Google Scholar] [Crossref]

33. Mengistu, B. A., Getnet, K., Mebratu, A. S., & Fenta, M. D. (2024). Occurrence, multidrug resistance and potential risk factors for Staphylococcus aureus infection at worker-animal and working equipment interfaces: A systematic review and meta-analysis of the Ethiopian literature. Frontiers in Public Health, 12, 1403012. [Google Scholar] [Crossref]

34. Mlynarczyk-Bonikowska, B., Kowalewski, C., Krolak-Ulinska, A., & Marusza, W. (2022). Molecular mechanisms of drug resistance in Staphylococcus aureus. International Journal of Molecular Sciences, 23(15), 8088. [Google Scholar] [Crossref]

35. Nhatsave, N., Garrine, M., Messa, A. J., Massinga, A. J., Cossa, A., Vaz, R., Ombí, A., Zimba, T. F., Alfredo, H., Mandomando, I., & Tchamo, C. (2021). Molecular characterization of Staphylococcus aureus isolated from raw milk samples of dairy cows in Manhica District, Southern Mozambique. Microorganisms, 9(8), 1684. [Google Scholar] [Crossref]

36. Normanno, G., La Salandra, G., Dambrosio, A., Quaglia, N. C., Corrente, M., Parisi, A., Santagada, G., Firinu, A., Crisetti, E., & Celano, G. V. (2007). Occurrence, characterization and antimicrobial resistance of enterotoxigenic Staphylococcus aureus isolated from meat and dairy products. International Journal of Food Microbiology, 115(3), 290–296. [Google Scholar] [Crossref]

37. Oliveira, R., Pinho, E., Almeida, G., Azevedo, N. F., & Almeida, C. (2022). Prevalence and diversity of Staphylococcus aureus and staphylococcal enterotoxins in raw milk from Northern Portugal. Frontiers in Microbiology, 13, 846653. [Google Scholar] [Crossref]

38. Paramasivam, R., Gopal, D. R., Dhandapani, R., Subbarayalu, R., Elangovan, M. P., Prabhu, B., Veerappan, V., Nandheeswaran, A., Paramasivam, S., & Muthupandian, S. (2023). Is AMR in dairy products a threat to human health? An updated review on the origin, prevention, treatment, and economic impacts of subclinical mastitis. Infection and Drug Resistance, 16, 155–178. [Google Scholar] [Crossref]

39. Patel, K., Godden, S. M., Royster, E. E., Crooker, B. A., Johnson, T. J., Smith, E. A., & Sreevatsan, S. (2021). Prevalence, antibiotic resistance, virulence and genetic diversity of Staphylococcus aureus isolated from bulk tank milk samples of U.S. dairy herds. BMC Genomics, 22, 367 [Google Scholar] [Crossref]

40. Pekana, A., & Green, E. (2018). Antimicrobial resistance profiles of Staphylococcus aureus isolated from meat carcasses and bovine milk in abattoirs and dairy farms of the Eastern Cape, South Africa. International Journal of Environmental Research and Public Health, 15(10), 2223. [Google Scholar] [Crossref]

41. Peralta, O. A., Carrasco, C., Vieytes, C., Tamayo, M. J., Muñoz, I., Sepúlveda, S., ... & Tadich, T. A. (2020). Safety and efficacy of a mesenchymal stem cell intramammary therapy in dairy cows with experimentally induced Staphylococcus aureus clinical mastitis. Scientific Reports, 10(1), 2843. [Google Scholar] [Crossref]

42. Pereira, U. P., Oliveira, D. G. S., Mesquita, L. R., Costa, G. M., & Pereira, L. J. (2011). Efficacy of Staphylococcus aureus vaccines for bovine mastitis: A systematic review. Veterinary Microbiology, 148(2–4), 117–124. [Google Scholar] [Crossref]

43. Petersson-Wolfe, C. S., Mullarky, I. K., & Jones, G. M. (2010). Staphylococcus aureus mastitis: Cause, detection, and control. Virginia Cooperative Extension. [Google Scholar] [Crossref]

44. Phiri, B. S. J., Hang’ombe, B. M., Mulenga, E., Mubanga, M., Maurischat, S., Wichmann-Schauer, H., Schaarschmidt, S., & Fetsch, A. (2022). Prevalence and diversity of Staphylococcus aureus in the Zambian dairy value chain: A public health concern. International Journal of Food Microbiology, 375, 109737. [Google Scholar] [Crossref]

45. Piepers, S., De Visscher, A., & Haesebrouck, F. (2022). Vaccination as a tool to combat Staphylococcus aureus mastitis in dairy cows: A systematic review and meta-analysis. Journal of Dairy Science, *105*(7),6225-6245. https://doi.org/10.3168/jds.2021-21567 [Google Scholar] [Crossref]

46. Price, L. B., Stegger, M., Hasman, H., Aziz, M., Larsen, J., Andersen, P. S., Pearson, T., Waters, A. E., Foster, J. T., & Schupp, J. (2012). Staphylococcus aureus CC398: Host adaptation and emergence of methicillin resistance in livestock. mBio, 3(1), e00305-11. [Google Scholar] [Crossref]

47. Qu, Y., Zhao, H., Nobrega, D. B., Cobo, E. R., Han, B., Zhao, Z., Li, S., Li, M., Barkema, H. W., & Gao, J. (2019). Molecular epidemiology and distribution of antimicrobial resistance genes of Staphylococcus species isolated from Chinese dairy cows with clinical mastitis. Journal of Dairy Science, 102(2), 1571–1583. [Google Scholar] [Crossref]

48. Rainard, P., & Foucras, G. (2018). A critical appraisal of probiotics for mastitis control. Frontiers in Veterinary Science, 5, 251. [Google Scholar] [Crossref]

49. Reed, S. G., Bertholet, S., Coler, R. N., & Friede, M. (2009). New horizons in adjuvants for vaccine development. Trends in Immunology, 30(1), 23–32. [Google Scholar] [Crossref]

50. Rollin, E., Dhuyvetter, K. C., & Overton, M. W. (2015). The cost of clinical mastitis in the first 30 days of lactation: An economic modeling tool. Preventive Veterinary Medicine, 122(3), 257–264. [Google Scholar] [Crossref]

51. Roy, M. C., Chowdhury, T., Hossain, M. T., Hasan, M. M., Zahran, E., Rahman, M. M., Zinnah, K. M. A., Rahman, M. M., & Hossain, F. M. A. (2024). Zoonotic linkage and environmental contamination of Methicillin-resistant Staphylococcus aureus (MRSA) in dairy farms: A one health perspective. One Health, 18, 100680. https://doi.org/10.1016/j.onehit.2024.100680 [Google Scholar] [Crossref]

52. Ruegg, P. L. (2017). A 100-year review: Mastitis detection, management, and prevention. Journal of Dairy Science, 100(12), 10381-10397. https://doi.org/10.3168/jds.2017-13023 [Google Scholar] [Crossref]

53. Russo, N., Fazio, N. A., Licitra, F., Gajewska, J., Stamilla, A., Salonia, R., Chajecka-Wierzchowska, W., Randazzo, C. L., Caggia, C., Antoci, F., & Cascone, G. (2025). Clonality, virulence genes, and antimicrobial resistance of dairy ruminants in mastitic milk-associated Staphylococcus aureus in Sicily. Antibiotics, 14(2), 188. [Google Scholar] [Crossref]

54. Schmidt, T., Kock, M. M., & Ehlers, M. M. (2017). Molecular characterization of Staphylococcus aureus isolated from bovine mastitis and close human contacts in South African dairy herds: Genetic diversity and inter-species host transmission. Frontiers in Microbiology, 8, 511. [Google Scholar] [Crossref]

55. Seegers, H., Fourichon, C., & Beaudeau, F. (2003). Production effects related to mastitis and mastitis economics in dairy cattle herds. Veterinary Research, 34(5), 475–491. [Google Scholar] [Crossref]

56. Silbergeld, E. K., Graham, J., & Price, L. B. (2008). Industrial food animal production, antimicrobial resistance, and human health. Annual Review of Public Health, 29, 151–169. [Google Scholar] [Crossref]

57. Silva, V., Araújo, S., Monteiro, A., Eira, J., Pereira, J. E., Maltez, L., Igrejas, G., Lemsaddek, T. S., & Poeta, P. (2023). Staphylococcus aureus and MRSA in livestock: Antimicrobial resistance and genetic lineages. Microorganisms, 11(1),124 [Google Scholar] [Crossref]

58. Straub, C., Taylor, W., French, N. P., Murdoch, D. R., Priest, P., Anderson, T., & Scott, P. (2024). Zoonotic transmission of asymptomatic carriage Staphylococcus aureus on dairy farms in Canterbury, New Zealand. Microbial Genomics, 10(12), 001318. https://doi.org/10.1099/mgen.0.001318 [Google Scholar] [Crossref]

59. Titze, I., Lehnherr, T., Lehnherr, H., & Krömker, V. (2020). Efficacy of bacteriophages against Staphylococcus aureus isolates from bovine mastitis. Pharmaceuticals, 13(3), 35. [Google Scholar] [Crossref]

60. Vanderhaeghen, W., Piepers, S., Leroy, F., Van Coillie, E., Haesebrouck, F., & De Vliegher, S. (2015). Invited review: Effect, persistence, and virulence of coagulase-negative Staphylococcus species associated with ruminant udder health. Journal of Dairy Science, 98(8), 5279-5294. https://doi.org/10.3168/jds.2014-9205 [Google Scholar] [Crossref]

61. Veloo, Y., Abu Thahir, S. S., Zakaria, Z., Abdul Rahman, S., Mansor, R., & Rajendiran, S. (2025). A scoping review unveiling antimicrobial resistance patterns in the environment of dairy farms across Asia. Antibiotics, 14(5), 436 [Google Scholar] [Crossref]

62. World Organisation for Animal Health. (2023). Terrestrial Animal Health Code: Section 6.10. on responsible and prudent use of antimicrobial agents in veterinary medicine. https://www.woah.org/en/what-we-do/standards/codes-and-manuals/terrestrial-code-online-access/ [Google Scholar] [Crossref]

63. Zaatout, N., Ayachi, A., & Kecha, M. (2020). Staphylococcus aureus persistence properties associated with bovine mastitis and alternative therapeutic modalities. Journal of Applied Microbiology, 129(5), 1102–1119. [Google Scholar] [Crossref]

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