Protective effects of Resveratrol Against Hydrogen Peroxide Induced Oxidative Stress in Model Organism Tribolium castaneum (Herbst, 1797)

Oxidative stress is a biological condition characterized by an imbalance between free radicals and antioxidants in living organisms. It is triggered by various factors, including environmental pollution, toxicants, radiation. Prolonged accumulation of reactive oxygen species (ROS) in the body leads to several chronic pathological conditions. Natural plant-derived polyphenolic compounds act as potent antioxidants by enhancing endogenous defense system and scavenging ROS, thereby reducing cellular damage. Resveratrol, a polyphenolic compound found in peanuts and berries, is known for its strong antioxidant properties. In the present study, the red flour beetle, Tribolium castaneum, was selected as an alternative model organism to evaluate the effects of resveratrol under oxidative stress conditions. DPPH scavenging activity, oxidative stress markers (lipid peroxidation and protein carbonyl) and cell viability were assessed in larvae oxidative stress induced by H₂O₂ for 24 h, followed by topical post treated with resveratrol (1mM). In-vitro DPPH assay showed lower IC50 for resveratrol (16.24 µg/ml) than ascorbic acid (25.96 µg/ml), indicating that resveratrol possesses stronger antioxidant activity. In-vivo, DPPH scavenging activity significantly increased after resveratrol treatment (87.83%) compared to control (83.13%) and stress- induced larvae (81.37%) at 48 h (p < 0.001). There was a significant increase in lipid peroxidation and protein carbonyl levels in stress-induced larvae compared to the control and resveratrol treated larvae (p < 0.001). MTT assay showed reduced cell viability in H₂O₂-exposed larvae, which was restored by resveratrol. Further studies are needed to elucidate genetic molecular mechanisms of resveratrol in preclinical models at different concentrations to ensure safety and minimize potential adverse effects.

Free radicals, Oxidative stress marker, Resveratrol, Cell viability, Tribolium castaneum

1. Afaq, F., Adhami, V.M., & Ahmad, N. (2003). Effects of phytochemicals on cellular pathways. Toxicology and Applied Pharmacology, 186(1), 28–37. https://doi.org/10.1016/S0041-008X(02)00014-5 [Google Scholar] [Crossref]

2. Akinwumi, B.C., Bordun, K.M., & Anderson, H.D. (2018). Biological activities of stilbenoids. International Journal of Molecular Sciences, 19, 792. https://doi.org/10.3390/ijms19030792 [Google Scholar] [Crossref]

3. Ayala, A., Muñoz, M. F., & Argüelles, S. (2014). Lipid peroxidation: Production, metabolism, and signalling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity, 2014, 360438. https://doi.org/10.1155/2014/360438 [Google Scholar] [Crossref]

4. Bingsohn, L., Knorr, E., & Vilcinskas, A. (2016). The model beetle Tribolium castaneum as an early warning system for transgenerational epigenetic side effects. Comparative Biochemistry and Physiology Part C, 18, 57–64. https://doi.org/10.1016/j.cbpc.2016.03.002 [Google Scholar] [Crossref]

5. Bozsányi, S., Rodrigues, R. C. G., Acquah, R., Tracy, E. C., Murphy, S. P., Tracy, J., ... & Paragh, G. (2026). Acute UV response of early erythema and late edema in SKH1 mice. Journal of Photochemistry and Photobiology B: Biology, 113369. [Google Scholar] [Crossref]

6. Campbell, J. F., Athanassiou, C. G., Hagstrum, D. W., & Zhu, K. Y. (2022). Tribolium castaneum: a model insect for fundamental and applied research. Annual review of entomology, 67(1), 347-365. [Google Scholar] [Crossref]

7. Catalani, E., Del Quondam, S., Brunetti, K., Cherubini, A., Bongiorni, S., et al. (2023). Neuroprotective role of plumbagin on eye damage in Drosophila melanogaster. Biomedicine & Pharmacotherapy, 166, 115298. [Google Scholar] [Crossref]

8. Chaubey, M.K. (2008). Fumigant toxicity of essential oils against Callosobruchus chinensis. Journal of Oleo Science, 57(3), 171–179. https://doi.org/10.5650/jos.57.171 [Google Scholar] [Crossref]

9. Coats, J. G., Maktabi, B., Abou‐Dahech, M. S., & Baki, G. (2021). Blue light protection, part II—Ingredients and performance testing methods. Journal of Cosmetic Dermatology, 20(3), 718-723. [Google Scholar] [Crossref]

10. Dalle-Donne, I., Rossi, R., Giustarini, D., Milzani, A., & Colombo, R. (2003). Protein carbonyl groups as biomarkers of oxidative stress. Clinica Chimica Acta, 329(1–2), 23–38. https://doi.org/10.1016/S0009-8981(03)00003-2 [Google Scholar] [Crossref]

11. Danieli, M. G., Antonelli, E., Piga, M. A., Cozzi, M. F., Allegra, A., & Gangemi, S. (2023). Oxidative stress, mitochondrial dysfunction, and respiratory chain enzyme defects in inflammatory myopathies. Autoimmunity Reviews, 22, 103308. https://doi.org/10.1016/j.autrev.2023.103308 [Google Scholar] [Crossref]

12. Devasagayam, T. P., & Tarachand, U. (1987). Decreased lipid peroxidation in the rat kidney during gestation. Biochemical and Biophysical Research Communications, 145(1), 134–138. https://doi.org/10.1016/0006-291X(87)91363-5 [Google Scholar] [Crossref]

13. Elsaesser, A., & Howard, C. V. (2012). Toxicology of nanoparticles. Advanced Drug Delivery Reviews, 64(2), 129–137. https://doi.org/10.1016/j.addr.2011.09.001 [Google Scholar] [Crossref]

14. Forman, H.J., & Zhang, H. (2021). Targeting oxidative stress in disease. Nature Reviews Drug Discovery, 20(9), 689–709. https://doi.org/10.1038/s41573-021-00233-1 [Google Scholar] [Crossref]

15. Hermanto, F., Haq, F.A., & Khasanah, R. (2024). Effect of resveratrol on antioxidant activity and antimalarial action. Journal of Advanced Pharmaceutical Technology & Research, 15(4), 359–363. https://doi.org/10.4103/JAPTR.JAPTR_144_24 [Google Scholar] [Crossref]

16. Huang, Y. T., Chen, Y. Y., Lai, Y. H., Cheng, C. C., Lin, T. C., Su, Y. S., Liu, C. H., & Lai, P. C. (2016). Resveratrol alleviates the cytotoxicity induced by the radiocontrast agent, ioxitalamate, by reducing the production of reactive oxygen species in HK-2 human renal proximal tubule epithelial cells in vitro. International journal of molecular medicine, 37(1), 83-91. https://doi.org/10.3892/ijmm.2015.2404 [Google Scholar] [Crossref]

17. Karakuş, H. D., Doğan, S., Yılmaz Kardaş, B., & Diken, M. E. (2026). In vivo developmental and antioxidant effects of some Salvia species on Drosophila melanogaster. Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 28(1), 212–231. https://doi.org/10.25092/baunfbed.1746986 [Google Scholar] [Crossref]

18. Lee, T.H., Seo, J.O., Baek, S.H., & Kim, S.Y. (2014). Effects of resveratrol on melanin synthesis. Biomolecules & Therapeutics, 22(1), 35–40. [Google Scholar] [Crossref]

19. Levine, R. L., Garland, D., Oliver, C. N., Amici, A., Climent, I., Lenz, A. G., Ahn, B. W., Shaltiel, S., & Stadtman, E. R. (1990). Determination of carbonyl content in oxidatively modified proteins. Methods in Enzymology, 186, 464–478. https://doi.org/10.1016/0076-6879(90)86141-H [Google Scholar] [Crossref]

20. Li, Z., Chen, X., Liu, G., Li, J., Zhang, J., Cao, Y., & Miao, J. (2021). Antioxidant activity of resveratrol and polydatin. Molecules, 26(24), 7574. https://doi.org/10.3390/molecules26247574 [Google Scholar] [Crossref]

21. Lushchak, V. I. (2014). Free radicals, reactive oxygen species, oxidative stress and its classification. Chemico-Biological Interactions, 224, 164–175. https://doi.org/10.1016/j.cbi.2014.10.016 [Google Scholar] [Crossref]

22. Murray, A., Palmer, D., Bennett, D., Dwarampudi, V., De Pedro, V., & Magalhães, J.A. (2020). Permeabilization of Drosophila larvae. Fly, 14(1–4), 29–33. https://doi.org/10.1080/19336934.2020.1724051 [Google Scholar] [Crossref]

23. Pandey, K. B., & Rizvi, S. I. (2009). Protective effect of resveratrol on formation of membrane protein carbonyls and lipid peroxidation in erythrocytes subjected to oxidative stress. Applied Physiology, Nutrition, and Metabolism, 34(6), 1093–1097. https://doi.org/10.1139/H09-115 [Google Scholar] [Crossref]

24. Pham-Huy, L.A., He, H., & Pham-Huy, C. (2008). Free radicals and antioxidants in health. International Journal of Biomedical Science, 4(2), 89–96. [Google Scholar] [Crossref]

25. Rogina, B., & Tissenbaum, H.A. (2024). SIRT1, resveratrol, and aging. Frontiers in Genetics, 15, 1393181. https://doi.org/10.3389/fgene.2024.1393181 [Google Scholar] [Crossref]

26. Rollin, B. E. (2003). Toxicology and animal ethics. Toxicologic Pathology, 31, 128–131. [Google Scholar] [Crossref]

27. Salehi, B., Mishra, A. P., Nigam, M., Sener, B., Kilic, M., Sharifi-Rad, M., Fokou, P. V. T., Martins, N., & Sharifi-Rad, J. (2018). Resveratrol: A double-edged sword in health benefits. Biomedicines, 6(3), 91. https://doi.org/10.3390/biomedicines6030091 [Google Scholar] [Crossref]

28. Saputra, F., Kishida, M., & Hu, S.Y. (2024). Oxidative stress in zebrafish visual development. Scientific Reports, 14, 22020. https://doi.org/10.1038/s41598-024-72932-9 [Google Scholar] [Crossref]

29. Schröder, R., Beermann, A., Wittkopp, N., & Lutz, R. (2008). Tribolium castaneum as a model organism. Development Genes and Evolution, 218(3–4), 119–126. [Google Scholar] [Crossref]

30. Seung Yun Lee, et al. (2022). Alternative experimental approaches to reduce animal use. Journal of Drug Delivery Science and Technology, 68, 103131. [Google Scholar] [Crossref]

31. Stadtman, E. R. (2002). Importance of individuality in oxidative stress and aging. Free Radical Biology and Medicine, 33(5), 597–604. https://doi.org/10.1016/S0891-5849(02)00904-8 [Google Scholar] [Crossref]

32. Subedi, R. P., Vartak, R. R., & Kale, P. G. (2017). Management of stress exerted by hydrogen peroxide in Drosophila melanogaster using Abhrak bhasma. Journal of Applied Pharmaceutical Science, 7(12), 065-071. [Google Scholar] [Crossref]

33. Tribolium castaneum: A model insect for research. (2022). Annual Review of Entomology, 67(1). https://doi.org/10.1146/annurev-ento-080921-075157 [Google Scholar] [Crossref]

34. Veerasham, C. (2012). Natural products as drug sources. Journal of Advanced Pharmaceutical Technology & Research, 3, 200. [Google Scholar] [Crossref]

35. Wu, Q.J., Zhang, T.N., Chen, H.H., et al. (2022). The sirtuin family in health and disease. Signal Transduction and Targeted Therapy, 7, 402. [Google Scholar] [Crossref]

36. Zhao, H. (2015). Phenolic compounds in food processing. In Processing and Impact on Active Components in Food (pp. 533–539). [Google Scholar] [Crossref]

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