Antimicrobial Activity of Ocimumgratissimum Extract Against Bacterial Isolates Associated with Spoilt Tomatoes Sold in Lafia Metropolis
- September 1, 2020
- Posted by: RSIS Team
- Categories: Botany and Microbiology, IJRIAS
International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume V, Issue VI, June 2020 | ISSN 2454-6194
Peter, U.U.1, Mfonido, I.B.2, Gladys, A.A.1, Naja’atu, S.H.1, Janet, C.G.1, Maryam, A.O.1, Kosiso, V.A.1 and Ogadegbor, A.O.1
1Department of Microbiology, Federal University of Lafia, Nigeria
2Department of Microbiology, University of Uyo, Nigeria
*Corresponding author
Abstract:- This study was conducted to investigate bacterial isolates associated with spoilt tomato fruits (Lycopersicunesculentum) obtained in Lafia metropolis, Northern Nigeria, and their susceptibility pattern on extract of Ocimumgratissimum. A total of 75 pieces of spoilt tomato fruits were purchased from Alamis, Kasuwan tomato, and Modern markets. Samples obtained were transported to the laboratory using sterile polyethylene bags. Spread plate methods were adopted for isolation. Colonies from each agar plates were examined both macroscopically and microscopically. Two different extracts, ethanol and methanol were obtained from Ocimumgratissimum. Agar well diffusion and micro-broth dilution technique were used for antibacterial susceptibility test and minimum inhibitory concentration, respectively. Five species of bacteria were identified of which Staphylococcus aureus was the dominant organism with percentage frequency of 19 (30.2%), Escherichia coli15 (23.8%), Salmonella spp12 (19.0%), Proteusspp9 (14.3%), and the least was Pseudomonasspp8 (12.7%). The methanol and ethanol extract of O. gratissimum showed activity against the test organisms. S. aureus was more susceptible to the extract with a mean zone of inhibition of 21.5±0.7mm and 23±2.5mm, respectively. The least activity was recorded in E. coli and Salmonellaspp with a mean zone of inhibition of 13.5±0.7mm and 2.5±0.7mm, respectively, in 100mg/mL.The mean zone of inhibition differs significantly at P<0.05 against the test organisms. The minimum inhibitory concentration of O. Gratissimum against the test organisms in ethanol and methanol extract was between 0.19-12.5mg/mL and 0.39-50mg/mL.O. gratissimum could be a potential drug for the treatment of infections. Having identified the presence of harmful bacteria such as S. aureus, Salmonellaspp, Pseudomonasspp Proteusspp Escherichia coli in spoilt tomatoes, the consumption of spoilt and unhealthy tomatoes among members of the populace should be discouraged.
Key words: Tomato, Ocimumgratissimum, Inhibitory, L.esculentum, diffusion
