Nuerobehavioural Investigation of The Role/S of Moringa Oleifera On 3-Nitropropionic Acid Model of Huntington’s Disease
- September 30, 2019
- Posted by: RSIS
- Categories: Health Sciences, IJRSI, Medical Sciences
International Journal of Research and Scientific Innovation (IJRSI) | Volume VI, Issue IX, September 2019 | ISSN 2321–2705
O.F. Akinpelu1, P.D. Shalli1, O.J Ogundipe2, A.A. Akande1, F.E. Oladipupo1, S.O. Jimoh1 and L. G Ogungbe1
1Department of Anatomy, Faculty of Basic Medical Sciences, Olabisi Onabanjo University, Ikenne Campus, Ogun State, Nigeria
2Department of Physiological Sciences, Faculty of Basic Medical Sciences, Obafemi Awolowo University, Ile – Ife, Osun State, Nigeria
Abstract:
Background: Huntington’s disease (HD) is a neurodegenerative disorder characterized by motor impairment that contribute to the death of striatial neurons and psychiatric symptoms. Systemic administration of 3-Nitropropionic Acid (3-NP) cause selective striatial degeneration similar to that seen in HD. Recent studies clearly demonstrate that increase oxidative stress is one of the major deleterious even in the 3-NP-induced neurodegenerative process. Moringa oleifera (MO) has been studied for the anti-oxidant, anti-inflammatory properties. This study evaluated the Neurobehavioral role of MO following 3-NP model of Huntington’s disease.
Methodology: Thirty-two adult mice weighting between 25-32g were used and divided into four groups (A, B, C and D), Group A serves as the control (Received food and ad libitum), B (Received MO+3-NP), C (Received only 3-NP) and D (Received 3-NP + MO) 3-Nitropropionic Acid (20mg/kg for 7 days) were induced intraperitoneal to the animals. MO were administer orally (250mg/kg) for a period of 7 days. Weights of the animals were checked periodically using the Acucuscope weighing Balance. Neurobehavioral assessment was carried out after administration. Animals were sacrificed at the end of the experiment Neurotransmitters- Dopamine and Glutamate were assayed in tissue homogenates and the levels were quantified.
Results showed increased tissue levels of glutamate and dopamine and impaired neurobehavioural parameters in the Huntington’s group, while the preventive group showed some degree of preservation of the normal parameters.
Conclusion: 3-Np induced huntington’s disease model in mice as expected with the characteristics of increased tissue levels of glutamate and dopamine and impaired behavioural and locomotive indices.
Key words: huntington’s disease, 3-Nitropropionic acid, Moringa, Neurobehavioral & Neurotransmitter
