Susceptibility Pattern of Escherichia coli Isolated from Environmental Samples in Ondo State, Nigeria
- October 19, 2020
- Posted by: RSIS Team
- Categories: IJRIAS, Microbiology
International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume V, Issue X, October 2020 | ISSN 2454–6186
Cajethan Onyebuchi Ezeamagu1*, Osasere Joy Aganmwonyi 2
1, 2 Department of Microbiology, Babcock University, Ilishan.
Abstract- Escherichia coli is a notorious and important bacterium in nosocomial infections with significant morbidity and mortality in healthcare institutions due to multi-drug resistance challenges. In this study, susceptibility pattern of environmental isolates of E. coli was evaluated. Environmental samples from faecal and pond water sources collected from Akure, Ondo State were subjected to bacterial isolation. Escherichia coli was isolated and identified presumptively using eosin methylene blue agar. The susceptibility was evaluated using agar diffusion method according to CLSI guidelines. Fifty none duplicate isolates of E. coli with metallic sheen were obtained of which all were resistant to amoxicillin/clavulanate (AUG) and 6 %, 46 %, 4 %, 2 %, 20 %, 16 % to cefixime, ciprofloxacin, cefuroxime, gentamicin, nitrofurantion and ofloxacin respectively. Multidrug resistance was observed in among the species with 22% against eight antibiotics. The result of this study showed high rates of multidrug resistance of E. coli strains in environmental samples. Hence regular antimicrobial surveillance is essential to minimize the proliferation of resistant E. coli in our environment.
Keywords- Susceptibility, E. coli, Antibiotics, Resistance, Environmental samples
I. INTRODUCTION
Escherichia coli is a typical reservoir of the human and animal gut, however can likewise be found in habitats such as vegetation, soil and water. It is the main microbe causing urinary infections [1-3] and is among the most widely recognized microorganisms causing systemic [4], ear and wounds infections, as well as humans-associated complications [5, 6]. Additionally, E. coli is the most well-known cause of food and water-borne human disease that characterized by water stool worldwide and in evolving nations, causing numerous mortality in youngsters below five years [7].
