Phytochemical Screening and Total Phenolic Content in Phyllanthus Niruri and Mimosa Pudica Collected from Kuching, Sarawak (Malaysia)

Authors

Firus Musfirah Poli

Faculty of Applied Sciences, University Technology MARA Sarawak, Samarahan 2 Campus, 94300 Kota Samarahan, Sarawak, Malaysia (Malaysia)

Nurul Zawani Mohamad Zamri

Faculty of Applied Sciences, University Technology MARA Sarawak, Samarahan 2 Campus, 94300 Kota Samarahan, Sarawak, Malaysia (Malaysia)

Article Information

DOI: 10.51244/IJRSI.2025.120800415

Subject Category: Education

Volume/Issue: 12/9 | Page No: 4585-4590

Publication Timeline

Submitted: 2025-09-16

Accepted: 2025-09-24

Published: 2025-10-23

Abstract

Phyllanthus niruri and Mimosa pudica are herbal plants that have been traditionally used as medicine to treat numerous ailments. This study has identified the major phytochemicals and quantified the total phenolic content (TPC) in the ethanolic extract of the plants. Plant samples were collected from several locations in Kuching, Sarawak. They were cleaned, dried, and ground prior for analysis. Phytochemical screening revealed the presence of alkaloids, saponins, protein, amino acids, tannins, and flavonoids in P. niruri extract, while only alkaloids, saponins, and flavonoids were identified on M. pudica extract. Folin–Ciocalteu method was used to determine the TPC and the results expressed as mg gallic acid equivalents (GAE) per gram of extract. From the results, P. niruri extract (59.06 mg GAE/g) exhibited slightly higher phenolic content compared to M. pudica extract (44.29 mg GAE/g). These findings emphasize the phytochemical abundance and antioxidant ability of these plants, supporting their effectiveness as medicinal plants.

Keywords

Phyllanthus niruri, Mimosa pudica, phytochemicals, total phenolic content

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References

1. Basiru, A., Ibukun, E., Edobor, G., Ojo, O., & Onikanni, S. (2013). Qualitative and quantitative analysis of phytochemicals in Senecio biafrae leaf. International Journal of Inventions in Pharmaceutical Sciences, (1), 428-432. [Google Scholar] [Crossref]

2. Burkill, I. H. (1966). A dictionary of the economic products of the Malay Peninsula. [Google Scholar] [Crossref]

3. Calixto, J. B., Santos, A. R., Filho, V. C., & Yunes, R. A. (1998). A review of the plants of the genus Phyllanthus: their chemistry, pharmacology, and therapeutic potential. Medicinal research reviews, 18(4), 225-258. [Google Scholar] [Crossref]

4. Chopra, R. N., & Nayar, S. L. (1956). Glossary of Indian medicinal plants. Council of scientific and Industrial Research. [Google Scholar] [Crossref]

5. Dhar, M. L., & Dhar, M. M. (1968). Screening of Indian plants for biological activity. Part I. [Google Scholar] [Crossref]

6. Dharmaraj, S., Jamaludin, A. S., Razak, H. M., Valliappan, R., Ahmad, N. A., Harn, G. L., & Ismail, Z. (2006). The classification of Phyllanthus niruri Linn. according to location by infrared spectroscopy. Vibrational Spectroscopy, 41(1), 68-72. [Google Scholar] [Crossref]

7. Faujan, N. H., Rahim, Z. A., Rehan, M. M., & Haji Ahmad, F. B. (2015). Comparative Analysis of Phenolic Content and Antioxidative Activities of Eight Malaysian traditional vegetables. Malaysian Journal of Analytical Sciences, 19(3), 611 - 624. [Google Scholar] [Crossref]

8. Gandhiraya, N., Sriram, S., Meenaa, V., Srilakshmi, J. K., Sasikumar, C., & Rajeswari, R. (2009). Phytochemical Screening and Antimicrobial Activity of the Plant Extracts of Mimosa pudica L. Against Selected Microbes. Ethnobotanical Leaflets, 13(24), 618. [Google Scholar] [Crossref]

9. Harborne, A. J. (1998). Phytochemical methods: a guide to modern techniques of plant analysis. Springer Dordrecht. [Google Scholar] [Crossref]

10. Kaur, N., Kaur, B., & Sirhindi, G. (2017). Phytochemistry and pharmacology of Phyllanthus niruri L.: a review. Phytotherapy research, 31(7), 980-1004. [Google Scholar] [Crossref]

11. Mace, M. E. (1963). Histochemical localization of phenols in healthy and diseased tomato roots. Phytopathology, 16, 915-925. [Google Scholar] [Crossref]

12. Mahanta, M., & Mukherjee, A. K. (2001). Neutralisation of lethality, myotoxicity and toxic enzymes of Naja kaouthia venom by Mimosa pudica root extracts. Journal of ethnopharmacology, 75(1), 55-60. [Google Scholar] [Crossref]

13. Mandal, A. K., Pandey, A., Sah, R. K., Baral, A., & Sah, P. (2022). In Vitro Antioxidant and Antimicrobial Potency of Mimosa pudica of Nepalese Terai Region: Insight into L-Mimosine as an Antibacterial Agent. Evidence-Based Complementary and Alternative Medicine, 2022(1). [Google Scholar] [Crossref]

14. Patro, G., Bhattamisra, S. K., & Mohanty, B. K. (2016). Effects of Mimosa pudica L. leaves extract on anxiety, depression and memory. Avicenna journal of phytomedicine, 6(6), 696. [Google Scholar] [Crossref]

15. Raaman, N. (2006). Phytochemical techniques. New India Publishing Agency. [Google Scholar] [Crossref]

16. Ramakrishnan, S. (2004). Textbook of medical biochemistry. Orient Blackswan. [Google Scholar] [Crossref]

17. Ramandeep, K., Nahid, A., Neelabh, C., & Navneet, K. (2017). Phytochemical Screening of Phyllanthus niruri collected from Kerala Region and its Antioxidant and Antimicrobial Potentials. Journal of Pharmaceutical Sciences and Research, 9(8), 1312-1316. [Google Scholar] [Crossref]

18. Ripen, J. E., & Noweg, G. T. (2016). Economic valuation of medicinal plants in Jagoi Area, Bau, Malaysia. Procedia-Social and Behavioral Sciences, 224, 124-131. [Google Scholar] [Crossref]

19. Shaikh, S. M., & Patil, A. B. (2020). Phytochemical screening techniques and their applications. Pharma Research Journal, 7(1), 23-30. [Google Scholar] [Crossref]

20. Slinkard, K., & Singleton, V. L. (1977). Total phenol analysis: automation and comparison with manual methods. American journal of enology and viticulture, 28(1), 49-55. [Google Scholar] [Crossref]

21. Varnika, S., Ashish, S., & Imran, A. (2012). A review on ethnomedical and traditional uses of Mimosa Pudica (Chui-Mui). Int. Res. J. Pharm, 3(2), 41-44. [Google Scholar] [Crossref]

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