Biochemical Analysis of Lentil Seed and Plant Seedling Infected with Fusarium Oxysporum and Rhizoctonia Solani

The present research work entitled biochemical analysis of lentil seed and plant seedling infected with fusarium oxysporum and rhizoctonia solani was conducted during Rabi season 2024-25 at the laboratory of modi institute of technology,Kota Rajasthan. The counter parts (Like leaf,stem,fruite and seeds) of lentil were selected for the study. The experiment was conducted using a Completely Randomized Design (CRD) with three replications to evaluate these parts.
Lentil (Lens culinaris) is an important legume crop grown around the world, but its yield often decreases due to fungal infections from Rhizoctonia solani and Fusarium oxysporum, which lead to root rot and damping-off diseases. The biochemical changes that these infections cause in lentil seeds and seedlings are examined in this study. The levels of total protein, phenolic content, antioxidant enzyme activity (peroxidase, catalase, and superoxide dismutase), and lipid peroxidation were among the biochemical markers that were examined in both infected and healthy samples. The results showed that defense-related enzyme activity was noticeably increased in infected plants and oxidative stress indicators, indicating an activated defensive mechanism. This response suggests that lentil plants have developed a robust defense system to combat the effects of rot and damping-off diseases. Further investigation into the specific pathways involved in these biochemical responses could provide valuable insights for improving disease resistance in lentil crops.
Additionally, variations in the accumulation of secondary metabolites were noted, highlighting their possible function in plant defense. The metabolic reactions of lentils to fungal diseases are better understood thanks to this study, which could also help create efficient disease control plans. Using standard quantitative techniques of biochemical analysis, important components of lentil seeds, such as lipid, starch, protein, phenol, and related enzymes, such as α- and β-amylase, polyphenol oxidase, protease, and lipase, were examined in the seeds infected with Rhizoctonia solani and Fusarium oxysporum.

Lens culinaris medic, Protein, Fat, Starch

1. Alalsohoimy, S.A.; Sitohy; M.Z and El- Masry, R.A. (2007). Isolation and partial characterization of chickpea, lupine and lentil seed proteins. World Jour. of Agric. Sci., 3(1):123-129. [Google Scholar] [Crossref]

2. Bhatnagar K (1992) Investigation into blight of cumin (Cuminum cyminum L.) caused by Alternaria burnsii in Rajasthan with special emphasis on its management. Ph.D. Thesis, Univ. Raj., Jaipur. [Google Scholar] [Crossref]

3. Bahadur A, Singh DP, Singh UP (2005) Biochemical changes in peas infected with Erysiphe pisi. Indian J. Plant Pathol. 23(1-2): 31-36. [Google Scholar] [Crossref]

4. Boyle, JL; S. Aksay, S; Roufik, S; Ribereau, M; Mondor, M; Mondor, E; Farnworth and S.H. Rajamohamed. (2010). Comparison of the functional properties of pea, chickpea and lentil protein concentrates processed using ultrafiltration and isoelectric precipitation techniques. Food Res. Int., 43: 537-546. [Google Scholar] [Crossref]

5. Chakraborty U, Chakraborty N (2002) Altered metabolic response of tea (Camellia sinensis) following attack by Helopeltis theivora Waterhouse. J. Mycol. Pl. Pathol. 32(3): 379. [Google Scholar] [Crossref]

6. Choudhury, S., Mahapatra, S., Chakraborty, S. et al. (2025). Revitalizing the lentil crop by use of a native Trichoderma sp. for Fusarium wilt and collar rot disease management and plant growth promotion. BioControl (2025). https://doi.org/10.1007/s10526-025-10366-6 [Google Scholar] [Crossref]

7. Dube S, Shukla HS,Tripathi SC (1988) Changes in sugar and protein contents of wheat due to Aspergilli. India Phytopath. 4: 633-635. [Google Scholar] [Crossref]

8. Dhull, SB, Kinabo, J,Uebersax MA (2022) Nutrient profile and effect of processing methods on the composition and functional properties of lentils (Lens culinaris Medik)—A review. Legume Science, e156. https://doi.org/10.1002/leg3.156 [Google Scholar] [Crossref]

9. Foti, C., Zambounis, A., Bataka, E. P., Kalloniati, C., Panagiotaki, E., Nakas, C. T., Flemetakis, E., & Pavli, O. I. (2024). Metabolic Aspects of Lentil–Fusarium Interactions. Plants, 13(14), 2005. https://doi.org/10.3390/plants13142005 [Google Scholar] [Crossref]

10. FAO (Food and Agriculture Organization) (2021) Crop Production and Trade Data. Accessed September 18, 2021. http://www.fao.org/faostat/en/#home [Google Scholar] [Crossref]

11. Ghoshal TK, S. Dutta, SK Senapati, DC Deb (2004) Role of phenol contents in legume seeds and its effect on the biology of collosobrocnus chinensis. Ann. Pl. Prot. Sci. 12: 442-444. [Google Scholar] [Crossref]

12. Hill H (2022) Utilization of dry beans and other pulses as ingredients in diverse food products. In M. Siddiq & M. A. Uebersax (Eds.). Dry Beans and Pulses: Production, Processing, and Nutrition (Second ed.) (pp. 307–329). John Wiley & Sons. https://doi.org/10.1002/9781119776802.ch12 [Google Scholar] [Crossref]

13. Irum, S., Khalid, M. H. B., Hussain, T., Saeed, A., Haider, I., Ahmed, Z., ... & Elshikh, M. S. (2025). Comprehensive evaluation of agronomic traits, physiological responses, and gene expression in chickpea cultivars under fungal stress. Functional Plant Biology, 52(10) https://doi.org/10.1071/FP25100 [Google Scholar] [Crossref]

14. Jayaraman J (1981) Laboratory manual in biochemistry. Willey Eastern Ltd., New Delhi, pp. 96-97. [Google Scholar] [Crossref]

15. Jain AK, HS Yadav (2003) Biochemical constituents of finger millet genotypes associated with resistance to blast caused by Pyricularis grisea. Ann.Pl.Protec.Sci. 11: 70-74. [Google Scholar] [Crossref]

16. Joshi M, Timilsena Y, Adhikari B (2017) Global production, processing, and utilization of lentils: A review. Journal of Integrative Agriculture, 16, 2898–2913. https://doi.org/10.1016/S2095-3119(17)61793-3 [Google Scholar] [Crossref]

17. Kaur J,Dhillon M (1990) Biochemical alteration in groundnut leaves induced by Cercosporium personatum. Indian J. Mycol. Pl. Path. 19: 151-156. [Google Scholar] [Crossref]

18. Kushwaha KPS, Udit Narain (2005) Biochemical changes in pigeon pea leaves infested with Alternaria tenuissima. Ann. Pl. Protec. Sci. 13: 415-417. [Google Scholar] [Crossref]

19. Kushwaha KPS, Udit Narain (2005) Biochemical changes in pigeon pea leaves infested with Alternaria tenuissima. Ann. Pl. Protec. Sci. 13: 415-417. [Google Scholar] [Crossref]

20. Licia MP, Cintia MR, Mario DB, Guillermo RC (2006) Catalytic Properties of Lipase Extracts from Aspergillus niger, Food Technol. Biotechnol. 44(2): 247-252. [Google Scholar] [Crossref]

21. Mathur R. (1992) Pathological and physiological studies on some seed disorders in soybean. Ph.D. Thesis, Rajasthan, Univ. Jaipur. [Google Scholar] [Crossref]

22. Maccarrone M, Veldink GA, Vliegenthart JFG, Agro AF (1997) Ozone stress modulates amino oxidase and lipooxygenase expression in lentil (lens culinaris) seedlings. FEBS. Lett. 408, 241-244. [Google Scholar] [Crossref]

23. Maria de Mascena DM, Marcia Maria CM, Marcos A, Eduardo H, Jose L (1999) Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FSI. Rev. Microbiol., 30: 3714-3721. [Google Scholar] [Crossref]

24. Malik S, Kumar P, Panwar JDS, Rathi YPS (2002) Physiological and biochemical alterations induced by urdbean leaf crinkle virus in Vigna mung. Ann. Pl. Protec. Sci. 10: 91-94. [Google Scholar] [Crossref]

25. Maheshwari M, Uma S, Suresh and N Emmaneul (2006) Biochemical basis of resistance in rice hybrids and conventional varieties against brown planthopper. Ann. Pl. Protec. Sci. 14: 69-72. [Google Scholar] [Crossref]

26. Muthukumar A,Bhaskaran R, Eswaran A, Rajkumar M (2007) Studies on the biochemical properties of healthy and leaf spot-infected tuberose plants. Indian J. Hort. 64(2): 190-193. [Google Scholar] [Crossref]

27. Moldovan, O; Paucean, A; Vlaic, R; Bors,D.M and Muste, S. (2015). Preliminary assessment of the nutritional quality of two types of lentils (Lens culinaris Medik) by Near Infrared Reflectance Spectroscopy Technology (NIRS). Bulletin Uasvam Food Science and Technology 72(1). [Google Scholar] [Crossref]

28. Pustovoit GV, Borodin SG (1983) Harmfulness of grey rot of sunflower. Zashcia Rastenil. No. 91: 41. [Google Scholar] [Crossref]

29. Prasad BK, Prasad A, Shankar U, Kumar S (1988) Levels of change in carbohydrate in lablab bean FD5 CV S. seeds due to seed-borne Aspergillus niger. Indian J. Mycol. Pathol. 17: 145-148. [Google Scholar] [Crossref]

30. Parashar A, Lodha P (2007) Phenolic estimation in Foeniculum vulgare infected with Ramularia blight. Ann. Pl. Protec. Sci. 15: 396-398. [Google Scholar] [Crossref]

31. Qayyum, N.M.M., Butt, S.M., Anjum, M.F. and Nawaz, H. (2012). Composition analysis of some selected legumes for protein isolates recovery. The journal of animal & plant sciences, 22(4): 1156-1162. [Google Scholar] [Crossref]

32. Rathi AS, Parashar RD, Sindhan GS (1998) Biochemical changes in pea leaves due to powdery mildew infection. Ind. J. Mycol. and Plant Pathol. 28(3): 330-333. [Google Scholar] [Crossref]

33. Rao CN, Panwar VPS (2001) Biochemical plant factors affecting resistance to Atherigona spp. In maize. Ann. Pl. Protec. Sci. 9: 37-42. [Google Scholar] [Crossref]

34. Shukla HS, Dube KS, Tripathi SC (1988) Change in protein content of arhar (Cajanus cajan) due to fungal association. Legume Research, 11: 85-88. [Google Scholar] [Crossref]

35. Singh SK, Srivastava HP (1988) Phenolic changes in moth bean (Vigna aconintifolia (Jacq.) Marechal) during Macrophomia phaseolina infection. Indian J. Mycol. Pl. Pathol. 18: 314-316. [Google Scholar] [Crossref]

36. Singh D, Singh KG (1990) Occurrence of fungi in rubber of Malaysia. Indian Journal of Natural Rubber Research, 3: 64-65. [Google Scholar] [Crossref]

37. Singh P, Bhagat S, Ahmad SK (1990) Aflatoxin elaboration and nutrition deterioration in some pulse cultivars during infestation with A. flavus. J. Food Sci. Technol. (Mysore). 27(1): 60-62. [Google Scholar] [Crossref]

38. Sharma J (1992) Mycoflora of soybean seeds and their pathological effects. Ph.D. Thesis, Univ. Raj., Jaipur. [Google Scholar] [Crossref]

39. Singh HV (2000) Biochemical basis of resistance in Brassica species against downy mildew and white rust of mustard. Pl. Dis. Res. 15: 75-77. [Google Scholar] [Crossref]

40. Singh HV (2004) Biochemical transformation in Brassica spp. due to Peronospora parasitica infestation. Ann. Pl. Protec. Sci. 12: 301-304. [Google Scholar] [Crossref]

41. Singh, K.A., Kumar, P., Singh, J., Rani, R., Rani, A., Shukla, P. and Misra, P. (2014). Biochemical profiling of lentil (lens culinarismedik) germplasm at different growth stages. J. Biol. Eng. Res. & Rev 2 (1) 01-06. [Google Scholar] [Crossref]

42. Singh Anita "Quantification of Total Protein and Related Enzymes in Fusarium Wilt-Infected Lens culinaris medic." J Microbiol Pathol 5 (2021): 1-3. [Google Scholar] [Crossref]

43. Singh Anita "Quantification of Total lipid and Related Enzymes in Fusarium Wilt-Infected Lens culinaris medic." International Journal of Science and Research (IJSR) 11(2022): 187-189. DOI: 10.21275/SR22624135154 [Google Scholar] [Crossref]

44. Singh Anita and Varma Rashmi "Quantification of Total Phenol and Related Enzymes in Fusarium Wilt-Infected Lens culinaris medic." Indian journal of applied and pure biology 5 (2010): 269-271. [Google Scholar] [Crossref]

45. Singh Anita and Varma Rashmi "Quantification of Total Carbohydrate and Related Enzymes in Fusarium Wilt-Infected Lens culinaris medic." International journal of mendel 27 (2010): 49-51. [Google Scholar] [Crossref]

46. Sidhu JS, Zafar T, Benyathiar P, Nasir M (2022) Production, processing, and nutritional profile of chickpeas and lentils. In M. Siddiq & M. A. Uebersax (Eds.). Dry Beans and Pulses: Production, Processing, and Nutrition (Second ed.) (pp. 383–407). John Wiley & Sons. https://doi.org/10.1002/9781119776802.ch15 [Google Scholar] [Crossref]

47. Tiwari, N., Ahmed, S., Kumar, S., & Sarker, A. (2018). Fusarium Wilt: A Killer Disease of Lentil. InTech. DOI: 10.5772/intechopen.72508 [Google Scholar] [Crossref]

48. Tripathi AK, RK. Pandya, MLTripathi (2001) Effect of nitrogen, phosphorus, and potassium on stem gall disease and yield of coriander. Ann. Pl. Protec. Sci. 9: 337-339. [Google Scholar] [Crossref]

49. Jha, U. C., Bohra, A., Pandey, S., & Parida, S. K. (2020). Breeding, genetics, and genomics approaches for improving Fusarium wilt resistance in major grain legumes. Frontiers in Genetics, 11, 1001. https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2020.01001/full [Google Scholar] [Crossref]

50. Villaume C,Chandrasir V,Bau HM, Nicolas JP, Mejean L (1993) Effect du traitement technologique de preparation des proteines de soja sur la prise alimentaire du rat en croissance. Sci. Alim. 13:377-383. [Google Scholar] [Crossref]

51. Vidyasekaran P (2001) Physiology of disease resistance. In: Principles of Plant Pathology (1st Ed.). C.B.S. Publishers & Distributors, New Delhi, pp. 106-116. [Google Scholar] [Crossref]

52. Deyala M. Naguib (2019). Comparative lipid profiling for studying resistance mechanism against Fusarium wilt,Physiological and Molecular Plant Pathology, 108:101421https://doi.org/10.1016/j.pmpp.2019.101421. [Google Scholar] [Crossref]

53. Javeria Ayub et al., (2024). Effective biological control of Lentil (Lens culinaris) Fusarium wilt and plant growth promotion through native Rhizobacteria,Physiological and Molecular Plant Pathology, https://doi.org/10.1016/j.pmpp.2023.102203 [Google Scholar] [Crossref]

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