Molecular Characterization of Infectious Bronchitis Virus from Chickens in Sokoto State, Nigeria

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International Journal of Research and Scientific Innovation (IJRSI) | Volume VI, Issue VIII, August 2019 | ISSN 2321–2705

Molecular Characterization of Infectious Bronchitis Virus from Chickens in Sokoto State, Nigeria

Mungadi, H.U.1*, Mera, U.M.2, Daneji, A.I 2, Musa, U.3, Alkali, B.R 4, Bande, F.5, Shuaibu, A.B.4 Garba, S.1 and Yakubu Y.6

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1Veterinary Teaching Hospital, Usmanu Danfodiyo University Sokoto, Nigeria
2 Department of Veterinary Medicine Faculty of Veterinary Medicine Usmanu Danfodiyo University Sokoto, Nigeria
3Department of Veterinary Pathology, Faculty of Veterinary Medicine Usmanu Danfodiyo University Sokoto, Nigeria
4 Department of Veterinary Microbiology, Faculty of Veterinary Medicine Usmanu Danfodiyo University Sokoto, Nigeria
5 Department of Veterinary Services, Ministry of Animal Health and Fisheries Development Usman Faruk Secretariat, Sokoto State, Nigeria
6Department of Veterinary. Public Health and Preventive Medicine, Faculty of Veterinary Medicine Usmanu Danfodiyo University Sokoto, Nigeria

Abstract: – The aim of this research was to apply molecular technique to characterize infectious bronchitis virus (IBV) isolates and determine evolutionary relationship and genetic diversity of IBV strains from chickens in Sokoto State, Nigeria. Tracheal and cloacal swabs were taken from 150 chickens showing some clinical signs of infectious bronchitis (IB) for reverse transcription polymerase chain reaction (RT-PCR), gene sequencing and Phylogenetic analysis. Sequencing and phylogenetic analysis showed four out of the strains isolated were close to NGA/A11637/2006 strain previously reported in Northern Nigeria while one sequence showed closeness to a Chinese patridge/GD/S14/2003 strain which has not been reported previously in Nigeria. It was therefore concluded that,this study has provided an update on some circulating IBV strainsand the identification of two new strains of which are genetically distinct from all those earlier reported. The strains might have been introduced through migratory birds as well mutation or recombination of the previously reported IBADAN strain.
The recommendations were, there is need to know the pathogenicity of the circulatingIBV strains in the area and test the efficacy of available vaccines, also there is need to impose strict biosecurity measures in poultry houses in the study area to prevent contracting poultry diseases through migratory birds.
The Gen-Bank accession numbers for the full S1 gene sequences of TR/B/SK/05 and CL/YM/IS/15 are MK659577 and MK659578 respectively.

Key words: Molecular characterization, infectious bronchitis virus, S1 gene hypervariable region, Chickens, Sokoto state.