Molecular Detection of Avian Infectious Bronchitis Virus from Chickens in Sokoto State, Nigeria

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International Journal of Research and Scientific Innovation (IJRSI) | Volume VI, Issue VIII, August 2019 | ISSN 2321–2705

Molecular Detection of Avian Infectious Bronchitis Virus from Chickens in Sokoto State, Nigeria

Mungadi, H.U.1*, Mera, U.M.2, Musa, U.3, Shuaibu, A.B.4, Pilau, N.N.2, Ismai’la, M.S.5, Ahmed, A.6 Garba, S.1

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1 Veterinary Teaching Hospital, Usmanu Danfodiyo University Sokoto, Nigeria
2 Department of Veterinary Medicine, Faculty of Veterinary Medicine, Usmanu Danfodiyo University Sokoto, Nigeria
3Department of Veterinary Pathology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University Sokoto, Nigeria
4Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University Sokoto, Nigeria
5Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University Sokoto, Nigeria
6Department of Theriogenology and Animal Production, Faculty of Veterinary Medicine,Usmanu Danfodiyo University Sokoto, Nigeria
*Corresponding Author

Abstract: This research aimed at detection of infectious bronchitis virus (IBV) from chickens in Sokoto State, Nigeria using molecular technique. Three hundred (300) tracheal and cloacal swab samples from 150 chickens were taken in some selected areas of each of the four Agricultural zones of Sokoto State, namely; Sokoto, Tambuwal, Isah and Gwadabawa, pooled together to make 100 samples. The chickens manifested some clinical signs of infectious bronchitis (IB) and the samples were used for reverse transcription polymerase chain reaction (RT-PCR). Out of the 100 samples, 84 had RNA with the required purity and concentration. The overall result of the PCR showed 11(13.09%) positives. From all the tested exotic chickens, 12.50% were positive and out of all the tested indigenous ones, 13.50% were positive. Adults had 8.83% and young 16.00% positives. From the tested males, 17.65% were positive and from the tested females, 10.00 % were positive. The results for the zones showed that out of the tested chickens in Sokoto zone, 14.00% were positive, Tambuwal had 0.00% positives, Gwadabawa 8.33% and Isa 18.75%. In conclusion, the study has provided information on status of IBV in the study area which showed low prevalence of the virus at the period of sample collection. Continuous adoption of strategies to the field situation in Sokoto State was recommended. Indigenous poultry keepers should be enlightened and be encouraged by veterinarians to consider vaccination as a mean of prevention of poultry diseases like IB in the study area.
Key words: Infectious bronchitis virus (IBV), chickens, Sokoto State, polymerase chain reaction (PCR).