In-Vitro Antioxidant and Anti-Inflammatory Profile of the Stem Bark Extracts of Guiera Senegalensis J. F. Gmel

Medicinal Plants are Plants used for traditional medicines to treat ailments. Any part of the plant could be used as medicine. Medicinal plants have been discovered and used in traditional medicine practices since pre-historical times. A survey of traditional medicine man in Damaturu indicated that Guiera senegalensis is a plants used in the treatment of inflammation, wounds and cancer. The fresh samples (stem bark) of the plant was collected and successively extracted with n-hexane, ethyl acetate, methanol and water. Phytochemical constituents of Guiera senegalensis extracts revealed the presence of cardiac glycosides, flavonoids, saponins, phenols, tannins, alkaloids, sterols and terpenoids. The antioxidant potential was evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay, whereas membrane stabilization method was used to evaluate the anti-inflammatory potential of the extracts. The IC50 values obtained for the Guiera senegalensis extracts indicated that the methanol and water extracts exhibited higher antioxidant activities than the standard ascorbic acid. The extracts were found effective in inhibiting the heat induced haemolysis. Diclofenac Sodium salt used as standard drug at 100μg/ml offered 90.66% protection a significant (p<0.05) protection against damaging effect of heat solution. The results showed that all extracts from the plant offered a significant (p<0.05) protection against the damaging effect of hypotonic solution, Diclofenac sodium (100μg/ml), the standard drug offered a significant (p<0.05) protection (57.52%).

Phytochemical, Antioxidant, Anti-inflammatory

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